Rabbit Recombinant Monoclonal IL-33 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - Human, Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img434207.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847)")
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428310.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847)")
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428309.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847)")
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428308.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img428287.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (AB316847)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | ICC/IF | WB | IHC-P | IP | |
|---|---|---|---|---|---|
| Human | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Cytokine that binds to and signals through the IL1RL1/ST2 receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells (PubMed:16286016, PubMed:19841166). Involved in the maturation of Th2 cells inducing the secretion of T-helper type 2-associated cytokines (PubMed:17853410, PubMed:18836528). Also involved in activation of mast cells, basophils, eosinophils and natural killer cells (PubMed:17853410, PubMed:18836528). Acts as an enhancer of polarization of alternatively activated macrophages (PubMed:19841166). Acts as a chemoattractant for Th2 cells, and may function as an 'alarmin', that amplifies immune responses during tissue injury (PubMed:17853410, PubMed:18836528). Induces rapid UCP2-dependent mitochondrial rewiring that attenuates the generation of reactive oxygen species and preserves the integrity of Krebs cycle required for persistent production of itaconate and subsequent GATA3-dependent differentiation of inflammation-resolving alternatively activated macrophages (By similarity). In quiescent endothelia the uncleaved form is constitutively and abundantly expressed, and acts as a chromatin-associated nuclear factor with transcriptional repressor properties, it may sequester nuclear NF-kappaB/RELA, lowering expression of its targets (PubMed:21734074). This form is rapidely lost upon angiogenic or pro-inflammatory activation (PubMed:18787100).
Alternative names
C9orf26, IL1F11, NFHEV, IL33, Interleukin-33, IL-33, Interleukin-1 family member 11, Nuclear factor from high endothelial venules, IL-1F11, NF-HEV
Recommended products
Rabbit Recombinant Monoclonal IL-33 antibody. Carrier free. Suitable for Flow Cyt (Intra), ICC/IF and reacts with Transfected cell line - Human, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26367-2
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab316847 is the carrirer-free version of Anti-IL-33 antibody [EPR26367-2] ab316846.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img434207.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 100% methanol fixed, 0.1% Triton X-100 permeabilized HUVEC (human umbilical vein endothelial) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/500 dilution, followed by Goat anti-Rabbit (AlexaFluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution. Confocal image showing the nuclear and weak cytoplasmic staining (shown in green) is decreased after the treatment with IL-1β (5 ng/ml) for 24 hours in HUVEC cells. Nuclear DNA was labeled with DAPI (shown in blue).
Counterstained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A]-Microtubule Marker (Alexa Fluor® 594) at 1/200 dilution (shown in magenta).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
The reduction in IL-33 when stimulated with TNFa in HUVEC cells (specially the nuclear IL-33) (PMID: 18787100).
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428310.jpg "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HUVEC (human umbilical vein endothelial cell) treated with 500 pg/ml TNF-α for 24h and 300ng/ml BFA for 8h (Red) / Untreated HUVEC (Dotted Red) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/50 dilution (1 ug)/Red and Dotted Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
PMID: 18787100. Weaker staining occurred in HUVEC cells treated with 500 pg/ml TNF-α for 24h and 300ng/ml BFA for 8h compared to untreated HUVEC cells.
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428309.jpg "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized THP-1 (human monocytic leukemia monocyte) treated with 5ug/ml LPS and 50nM PMA for 24h (Red) / Untreated THP-1 (Dotted Red) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/50 dilution (1 ug)/Red and Dotted Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Stonger staining occurred in THP-1 treated with 5ug/ml LPS and 50nM PMA for 24h compared to untreated THP-1 cells.
![Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--flow-cytometry-intracellular-img428308.jpg "Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Flow Cytometry (Intracellular) - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Isotype (Left) / 293T cells transfected with a human IL-33 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/50000 dilution (0.001 ug)/Middle and Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells are co-stained with Myc tag conjugated to Alexa Fluor®647.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img428287.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HUVEC (human umbilical vein endothelial cell) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear and cytoplasmic staining in HUVEC cell line.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img428286.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)
This data was developed using Anti-IL-33 antibody [EPR26367-2] ab316846, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized THP-1 (human monocytic leukemia monocyte) cells labelling IL-33 with Anti-IL-33 antibody [EPR26367-2] ab316846 at 1/500 (0.994 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).
Confocal image showing nuclear and weak cytoplasmic staining in THP-1 treated with lipopolysaccharide (LPS, 5 ug/ml) and Phorbol-12-myristate-13-acetate (PMA, 50nM) for 24 hours.
Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5ug/ml) dilution (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/ml) dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847), expandable thumbnail](https://content.abcam.com/products/images/il-33-antibody-epr26367-2-bsa-and-azide-free-ab316847--immunocytochemistry-immunofluorescence-img428286.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-33 antibody [EPR26367-2] - BSA and Azide free (ab316847)")
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