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AB101443

Anti-IL-34 antibody [1D12]

5

(1 Review)

|

(14 Publications)

Mouse Monoclonal IL-34 antibody. Suitable for Flow Cyt, WB, ICC/IF and reacts with Human samples. Cited in 14 publications. Immunogen corresponding to Recombinant Fragment Protein within Human IL34 aa 1-250.

View Alternative Names

C16orf77, IL34, Interleukin-34, IL-34

3 Images
Flow Cytometry - Anti-IL-34 antibody [1D12] (AB101443)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-IL-34 antibody [1D12] (AB101443)

ab101443 staining IL-34 in K562 cells (green) at 1/200 by Flow cytometry. Negative control (purple).

Immunocytochemistry/ Immunofluorescence - Anti-IL-34 antibody [1D12] (AB101443)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-34 antibody [1D12] (AB101443)

ab101443, at 1/200 dilution, staining IL-34 in U251 cells by Immunofluorescence analysis (green). Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot - Anti-IL-34 antibody [1D12] (AB101443)
  • WB

Unknown

Western blot - Anti-IL-34 antibody [1D12] (AB101443)

All lanes:

Western blot - Anti-IL-34 antibody [1D12] (ab101443) at 1/500 dilution

All lanes:

IL-34-hIgGFc transfected HEK293 cell lysate

Predicted band size: 27 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

1D12

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human IL34 aa 1-250. The exact immunogen used to generate this antibody is proprietary information.

Q6ZMJ4

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of pro-inflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.
See full target information IL34

Publications (14)

Recent publications for all applications. Explore the full list and refine your search

iScience 28:112752 PubMed40538439

2025

Targeting the IL34-CSF1R axis improves metastatic renal cell carcinoma therapy outcome via immune-vascular crosstalk regulation.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Emanuelli,Wilfried Souleyreau,Tiffanie Chouleur,Bram Boeckx,Yasmine Pobiedonoscew,Lindsay Cooley,Marie-Alix Derieppe,Julie Martineau,Damien Ambrosetti,Jean-Christophe Bernhard,Catherine M Sawai,Diether Lambrechts,Thomas Mathivet,Andreas Bikfalvi

Heliyon 10:e33791 PubMed39055827

2024

IL-34 and its receptors as predictors of brain metastasis and prognosis in lung adenocarcinoma: Unveiling insights through bioinformatic and immunohistochemical investigations.

Applications

Unspecified application

Species

Unspecified reactive species

Jianxiong Geng,Shanqi Xu,Yingyue Cao,Fang Liu,Xingmei Ren,Dehai Che,Bo Pan,Yan Yu

European journal of immunology 53:e2250355 PubMed36991561

2023

Fibroblastic reticular cells provide a supportive niche for lymph node-resident macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Joshua D'Rozario,Konstantin Knoblich,Mechthild Lütge,Christian Pérez Shibayama,Hung-Wei Cheng,Yannick O Alexandre,David Roberts,Joana Campos,Emma E Dutton,Muath Suliman,Alice E Denton,Shannon J Turley,Richard L Boyd,Scott N Mueller,Burkhard Ludewig,Tracy S P Heng,Anne L Fletcher

PloS one 17:e0265725 PubMed35316296

2022

Pancreatic Ductal Adenocarcinoma (PDAC) circulating tumor cells influence myeloid cell differentiation to support their survival and immunoresistance in portal vein circulation.

Applications

Unspecified application

Species

Unspecified reactive species

Juan Pablo Arnoletti,Joseph Reza,Armando Rosales,Alberto Monreal,Na'im Fanaian,Suzanne Whisner,Milan Srivastava,Julia Rivera-Otero,Gongxin Yu,Otto Phanstiel Iv,Deborah A Altomare,Quang Tran,Sally A Litherland

JID innovations : skin science from molecules to population health 2:100112 PubMed35521044

2022

IL-34 Downregulation‒Associated M1/M2 Macrophage Imbalance Is Related to Inflammaging in Sun-Exposed Human Skin.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Horiba,Ryota Kami,Taiki Tsutsui,Junichi Hosoi

Cell reports. Medicine 2:100457 PubMed35028604

2021

Synergistic T cell signaling by 41BB and CD28 is optimally achieved by membrane proximal positioning within parallel chimeric antigen receptors.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Muliaditan,Leena Halim,Lynsey M Whilding,Benjamin Draper,Daniela Y Achkova,Fahima Kausar,Maya Glover,Natasha Bechman,Appitha Arulappu,Jenifer Sanchez,Katie R Flaherty,Jana Obajdin,Kristiana Grigoriadis,Pierre Antoine,Daniel Larcombe-Young,Caroline M Hull,Richard Buus,Peter Gordon,Anita Grigoriadis,David M Davies,Anna Schurich,John Maher

Scientific reports 9:16781 PubMed31727934

2019

Autocrine Production of Interleukin-34 Promotes the Development of Endometriosis through CSF1R/JAK3/STAT6 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiqing Lin,Junyan Ma,Yaomin Peng,Meina Sun,Kaihong Xu,Ruijin Wu,Jun Lin

Clinical & translational immunology 8:e1074 PubMed31417675

2019

Expression of IL-34 correlates with macrophage infiltration and prognosis of diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Osamu Noyori,Yoshihiro Komohara,Hesham Nasser,Masateru Hiyoshi,Chaoya Ma,Cheng Pan,Joaquim Carreras,Naoya Nakamura,Ai Sato,Kiyoshi Ando,Yutaka Okuno,Kisato Nosaka,Masao Matsuoka,Shinya Suzu

Scientific reports 8:418 PubMed29323162

2018

High co-expression of IL-34 and M-CSF correlates with tumor progression and poor survival in lung cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Muhammad Baghdadi,Hiraku Endo,Atsushi Takano,Kozo Ishikawa,Yosuke Kameda,Haruka Wada,Yohei Miyagi,Tomoyuki Yokose,Hiroyuki Ito,Haruhiko Nakayama,Yataro Daigo,Nao Suzuki,Ken-Ichiro Seino

Frontiers in aging neuroscience 9:244 PubMed28848420

2017

Studies on Colony Stimulating Factor Receptor-1 and Ligands Colony Stimulating Factor-1 and Interleukin-34 in Alzheimer's Disease Brains and Human Microglia.

Applications

Unspecified application

Species

Unspecified reactive species

Douglas G Walker,Tiffany M Tang,Lih-Fen Lue
View all publications

Product promise

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