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AB9622

Anti-IL-4 antibody

5

(3 Reviews)

|

(39 Publications)

Rabbit Polyclonal IL-4 antibody. Suitable for ELISA, WB, sELISA and reacts with Human samples. Cited in 39 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL4.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

1 Images
Western blot - Anti-IL-4 antibody (AB9622)
  • WB

Unknown

Western blot - Anti-IL-4 antibody (AB9622)

All lanes:

Western blot - Anti-IL-4 antibody (ab9622) at 0.2 µg/mL

All lanes:

Recombinant human IL4 protein (<a href='/en-us/products/unavailable/recombinant-human-il4-protein-ab73186'>ab73186</a>) at 0.0001 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 17 kDa

true

Exposure time: 2min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, sELISA, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IL4.

P05112

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>To detect hIL-4 by direct ELISA (using 100 μl/well antibody solution) a concentration of at least 0.5 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection.</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.</p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p>Can be used as Detection antibody with recommended pair.</p>" } } }

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4

Publications (39)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 28:0070 PubMed39262834

2024

Antibacterial Immunonegative Coating with Biocompatible Materials on a Nanostructured Titanium Plate for Orthopedic Bone Fracture Surgery.

Applications

Unspecified application

Species

Unspecified reactive species

Jeong-Won Lee,Jung-Ah Cho,Yoo Jin Roh,Min Ae Han,Je-Un Jeong,Sivakumar Allur Subramanian,Eunho Kang,Jiwoo Yeom,Chang-Hun Lee,Sung Jae Kim

Acta dermato-venereologica 104:adv35401 PubMed38348729

2024

Lesional Infiltration of Eosinophils, Basophils, and M2 Macrophages Expressing Eotaxin-1 and Eotaxin-3 in Granuloma Faciale, but not in Erythema Elevatum Diutinum.

Applications

Unspecified application

Species

Unspecified reactive species

Nozomi Akai,Takashi Hashimoto,Manami Okuzawa,Satoshi Okuno,Takahiro Satoh

Frontiers in microbiology 14:1197092 PubMed37389334

2023

Altered intestinal microbiota in children with bronchiolitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Bin Wu,Jian Wang,Yuan Tang,Jing Jiang,Xue-Mei Li

Journal of translational medicine 20:112 PubMed35255925

2022

Tumor-B-cell interactions promote isotype switching to an immunosuppressive IgG4 antibody response through upregulation of IL-10 in triple negative breast cancers.

Applications

Unspecified application

Species

Unspecified reactive species

Nicole J Toney,Lynn M Opdenaker,Kader Cicek,Lisa Frerichs,Christopher Ryan Kennington,Samuel Oberly,Holly Archinal,Rajasekharan Somasundaram,Jennifer Sims-Mourtada

PloS one 17:e0264775 PubMed35226700

2022

Four in one-Combination therapy using live Lactococcus lactis expressing three therapeutic proteins for the treatment of chronic non-healing wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Jere Kurkipuro,Igor Mierau,Thomas Wirth,Haritha Samaranayake,Wesley Smith,Hanna-Riikka Kärkkäinen,Mirka Tikkanen,Juha Yrjänheikki

Experimental dermatology 31:191-201 PubMed34358352

2021

Perivascular clusters of Th2 cells and M2 macrophages in allergic contact dermatitis to methylchloroisothiazolinone and methylisothiazolinone.

Applications

Unspecified application

Species

Unspecified reactive species

Anangélica R Virgens,Heliana F O Goes,Gabriel C de Carvalho,Anna Julia Pietrobon,Anna Cláudia C C Branco,Yasmim A L Ramos,Naiura V Pereira,Raquel L Orfali,Valéria Aoki,Luiz Fernando F da Silva,Mirian N Sotto,Vitor M S Dos Reis,Maria N Sato

Experimental and therapeutic medicine 20:3695-3702 PubMed32855721

2020

Impact of chitosan membrane culture on the expression of pro- and anti-inflammatory cytokines in mesenchymal stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yi,Xiongfeng Xu,Bo Qiu,Huajie Li

Brain, behavior, & immunity - health 5:100089 PubMed34589861

2020

Moderate noise associated oxidative stress with concomitant memory impairment, neuro-inflammation and neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Shukla,Kumar Vyonkesh Mani, Deepshikha,Sangeeta Shukla,Neeru Kapoor

eLife 9: PubMed32366355

2020

Role of IL-4 in bone marrow driven dysregulated angiogenesis and age-related macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Baba,Dai Miyazaki,Kodai Inata,Ryu Uotani,Hitomi Miyake,Shin-Ichi Sasaki,Yumiko Shimizu,Yoshitsugu Inoue,Kazuomi Nakamura

Cancer discovery 10:608-625 PubMed32046984

2020

Oncogenic KRAS-Driven Metabolic Reprogramming in Pancreatic Cancer Cells Utilizes Cytokines from the Tumor Microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

Prasenjit Dey,Jun Li,Jianhua Zhang,Surendra Chaurasiya,Anders Strom,Huamin Wang,Wen-Ting Liao,Frederick Cavallaro,Parker Denz,Vincent Bernard,Er-Yen Yen,Giannicola Genovese,Pat Gulhati,Jielin Liu,Deepavali Chakravarti,Pingna Deng,Tingxin Zhang,Federica Carbone,Qing Chang,Haoqiang Ying,Xiaoying Shang,Denise J Spring,Bidyut Ghosh,Nagireddy Putluri,Anirban Maitra,Y Alan Wang,Ronald A DePinho
View all publications

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