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AB269974

Anti-IL-4 antibody [OX81] - BSA and Azide free

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(1 Publication)

Mouse Recombinant Monoclonal IL-4 antibody. Carrier free. Suitable for ELISA and reacts with Rat samples. Cited in 1 publication.

View Alternative Names

Il-4, Il4, Interleukin-4, IL-4, B-cell IGG differentiation factor, B-cell growth factor 1, B-cell stimulatory factor 1, Lymphocyte stimulatory factor 1, BSF-1

1 Images
ELISA - Anti-IL-4 antibody [OX81] - BSA and Azide free (AB269974)
  • ELISA

Supplier Data

ELISA - Anti-IL-4 antibody [OX81] - BSA and Azide free (AB269974)

Direct ELISA antibody response curve using ab238471 at 0-5000 ng/ml and rat IL-4 at 1000 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) was used as the secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab238471).

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OX81

Isotype

IgG1

Carrier free

Yes

Reacts with

Rat

Applications

ELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p>0-5000 ng/ml</p>" } } }
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Product details

ab269974 is the carrier-free version of ab238471.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4.
See full target information Il4
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 9: PubMed39133648

2024

Chlorination of epithelial tight junction proteins by neutrophil myeloperoxidase promotes barrier dysfunction and mucosal inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ian M Cartwright,Liheng Zhou,Samuel D Koch,Nichole Welch,Daniel Zakharov,Rosemary Callahan,Calen A Steiner,Mark E Gerich,Joseph C Onyiah,Sean P Colgan
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com