Rabbit Recombinant Monoclonal IL-4I1/LAO antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Images
![Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--western-blot-img429607.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429661.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429660.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429659.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (AB317249)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IHC-P | ICC/IF | Flow Cyt (Intra) | IP | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Tested | Tested | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Rat | Dilution info - | Notes - |
Target data
Function
Secreted L-amino-acid oxidase that acts as a key immunoregulator (PubMed:32818467). Has preference for L-aromatic amino acids: converts phenylalanine (Phe), tyrosine (Tyr) and tryptophan (Trp) to phenylpyruvic acid (PP), hydroxyphenylpyruvic acid (HPP), and indole-3-pyruvic acid (I3P), respectively (PubMed:15383589). Also has weak L-arginine oxidase activity (By similarity). Acts as a negative regulator of anti-tumor immunity by mediating Trp degradation via an indole pyruvate pathway that activates the transcription factor AHR (PubMed:21469114, PubMed:28405502, PubMed:32818467). IL4I1-mediated Trp catabolism generates I3P, giving rise to indole metabolites (indole-3-acetic acid (IAA) and indole-3-aldehyde (I3A)) and kynurenic acid, which act as ligands for AHR, a ligand-activated transcription factor that plays important roles in immunity and cancer (By similarity). AHR activation by indoles following IL4I1-mediated Trp degradation enhances tumor progression by promoting cancer cell motility and suppressing adaptive immunity (PubMed:32818467). Also has an immunoregulatory function in some immune cell, probably by mediating Trp degradation and promoting downstream AHR activation: inhibits T-cell activation and proliferation, promotes the differentiation of naive CD4(+) T-cells into FOXP3(+) regulatory T-cells (Treg) and regulates the development and function of B-cells (PubMed:25778793, PubMed:29288206). Also regulates M2 macrophage polarization by inhibiting T-cell activation (PubMed:26599209). Also has antibacterial properties by inhibiting growth of Gram negative and Gram positive bacteria through the production of NH4(+) and H2O2 (By similarity).
Alternative names
Fig1, L-amino-acid oxidase, LAAO, LAO, Interleukin-4-induced protein 1, Protein Fig-1, IL4-induced protein 1, mIL4I1, mFIG1
Recommended products
Rabbit Recombinant Monoclonal IL-4I1/LAO antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR26368-90
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab317249 is the carrirer-free version of Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--western-blot-img429607.jpg "Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)")
Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)
IL-4I1/LAO Western blot staining using rabbit Anti-IL-4I1/LAO antibody
This data was developed using Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248, the same antibody clone in a different buffer formulation.
Low expression: heart, lung (PMID:16029492).
The identity of the higher MW band at approximately 110 kDa is unknown.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-IL-4I1/LAO antibody [EPR26368-90] (Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248) at 1/1000 dilution
Lane 1: Mouse testis tissue lysate at 20 µg with 5% NFDM/TBST
Lane 2: Mouse heart tissue lysate at 20 µg with 5% NFDM/TBST
Lane 3: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST
Lane 4: Untreated RAW 264.7 (mouse abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg with 5% NFDM/TBST
Lane 5: RAW 264.7 treated with /ml mouse IL-4 for 24 hours whole cell lysate at 20 µg with 5% NFDM/TBST
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 75 kDa, 124 kDa
Exposure time: 180s
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429661.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)
This data was developed using Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling IL-4I1/LAO with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 at 1/1000 (0.5 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Low expression: no staining on mouse cardiac muscle (PMID: 16029492). The section was incubated with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429660.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)
This data was developed using Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse testis tissue labeling IL-4I1/LAO with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 at 1/1000 (0.5 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on mouse testis (PMID: 16029492). The section was incubated with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249), expandable thumbnail](https://content.abcam.com/products/images/il-4i1-lao-antibody-epr26368-90-bsa-and-azide-free-ab317249--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img429659.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-4I1/LAO antibody [EPR26368-90] - BSA and Azide free (ab317249)
This data was developed using Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling IL-4I1/LAO with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 at 1/1000 (0.5 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on macrophages of mouse spleen (PMID: 19436310, PMID: 20683900). The section was incubated with Anti-IL-4I1/LAO antibody [EPR26368-90] ab317248 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
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