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AB288306

Anti-IL-4R antibody [EPR23671-21] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-4R antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

1 Images
Western blot - Anti-IL-4R antibody [EPR23671-21] - BSA and Azide free (AB288306)
  • WB

Lab

Western blot - Anti-IL-4R antibody [EPR23671-21] - BSA and Azide free (AB288306)

This data was developed using ab271041, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Low expression cell lines : MCF7, NCI-H460 (PMID : 28878029, 12429641).

Exposure time : 3 minutes

All lanes:

Western blot - Anti-IL-4R antibody [EPR23671-21] (<a href='/en-us/products/primary-antibodies/il-4r-antibody-epr23671-21-ab271041'>ab271041</a>) at 1/1000 dilution

Lane 1:

Ramos (human burkitts lymphoma b lymphocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

NCI-H460 (Human lung cancer cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 90 kDa

Observed band size: 140 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23671-21

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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AB243668

Human IL-4R ELISA Kit

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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: 100% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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