Rabbit Recombinant Monoclonal IL-4R antibody. Suitable for Flow Cyt and reacts with Human samples.
Images
![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435272.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080)")
![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435274.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080)")
![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435273.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (AB320080)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Abcam Recommends
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/5000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info - | Notes - |
Target data
Function
Receptor for both interleukin 4 and interleukin 13 (PubMed:17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
Alternative names
CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA
Recommended products
Rabbit Recombinant Monoclonal IL-4R antibody. Suitable for Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28312-39
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435272.jpg "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)")
Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-4R with ab320080 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti human CD19 conjugated to Alexa Fluor® 647.
Gated on viable cells.![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435274.jpg "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)")
Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)
Flow cytometric analysis of Daudi (human Burkitt's lymphoma lymphoblast) (Right) / MCF7 (human breast adenocarcinoma epithelial cell) (Left) cells labelling IL-4R with ab320080 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: MCF7 (PMID: 15331327)![Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080), expandable thumbnail](https://content.abcam.com/products/images/il-4r-antibody-epr28312-39-ab320080--flow-cytometry-img435273.jpg "Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)")
Flow Cytometry - Anti-IL-4R antibody [EPR28312-39] (ab320080)
Flow cytometric analysis of Raji (human Burkitt's lymphoma B lymphocyte) (Right) / NCI-H460 (human Large Cell Lung Cancer epithelial cell) (Left) cells labelling IL-4R with ab320080 at 1/5000 dilution (0.01ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Negative control: NCI-H460 (PMID:28878029)
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