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AB61099

Anti-IL-4R (phospho Y497) antibody

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(2 Publications)

Rabbit Polyclonal IL-4R phospho Y497 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL4R phospho Y497 aa 450-550.

View Alternative Names

CD124, IL4RA, 582J2.1, IL4R, Interleukin-4 receptor subunit alpha, IL-4 receptor subunit alpha, IL-4R subunit alpha, IL-4R-alpha, IL-4RA

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-IL-4R (phospho Y497) antibody (AB61099)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-IL-4R (phospho Y497) antibody (AB61099)

ICC/IF image of ab61099 (at a 1/500 dilution) stained HeLa cells, minus immunising peptide (left image) and plus immunising peptide (right image).

Western blot - Anti-IL-4R (phospho Y497) antibody (AB61099)
  • WB

Unknown

Western blot - Anti-IL-4R (phospho Y497) antibody (AB61099)

All lanes:

Western blot - Anti-IL-4R (phospho Y497) antibody (ab61099) at 1/500 dilution

Lane 1:

Extracts from 293 cells

Lane 2:

Extracts from 293 cells with immunizing phosphopeptide

Predicted band size: 90 kDa

Observed band size: 89 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human IL4R phospho Y497 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P24394

Specificity

Detects endogenous levels of IL4R only when phosphorylated at tyrosine 497 (corresponding to mouse tyrosine 500)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography, usingepitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for both interleukin 4 and interleukin 13 (PubMed : 17030238). Couples to the JAK1/2/3-STAT6 pathway. The IL4 response is involved in promoting Th2 differentiation. The IL4/IL13 responses are involved in regulating IgE production and, chemokine and mucus production at sites of allergic inflammation. In certain cell types, can signal through activation of insulin receptor substrates, IRS1/IRS2.. Soluble IL4R (sIL4R) inhibits IL4-mediated cell proliferation and IL5 up-regulation by T-cells.
See full target information IL4R phospho Y497
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell cycle (Georgetown, Tex.) 20:1923-1934 PubMed34437822

2021

Roles of Clara cell 10-kD protein and type 2 innate lymphoid cells in allergic rhinitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobo Long,Nan Wang,Xinhao Zhang

Journal of cell science 128:3781-95 PubMed26306492

2015

Essential role of endocytosis for interleukin-4-receptor-mediated JAK/STAT signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Kurgonaite,Hetvi Gandhi,Thomas Kurth,Sophie Pautot,Petra Schwille,Thomas Weidemann,Christian Bökel
View all publications
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Product promise

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