Anti-IL-5 [EPR20012-256] (Capture) antibody (ab242955) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect IL-5 in ELISA. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Images
![Sandwich ELISA - Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (AB242955), expandable thumbnail](https://content.abcam.com/products/images/il-5-antibody-epr20012-256-bsa-and-azide-free-capture-ab242955--sandwich-elisa-img181940.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (AB242955)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Homodimeric cytokine expressed predominantly by T-lymphocytes and NK cells that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:2653458, PubMed:9010276). Acts also on activated and resting B-cells to induce immunoglobulin production, growth, and differentiation (By similarity). Mechanistically, exerts its biological effects through a receptor composed of IL5RA subunit and the cytokine receptor common subunit beta/CSF2RB (PubMed:1495999, PubMed:22528658). Binding to the receptor leads to activation of various kinases including LYN, SYK and JAK2 and thereby propagates signals through the RAS-MAPK and JAK-STAT5 pathways respectively (PubMed:7613138).
Alternative names
Interleukin-5, IL-5, B-cell differentiation factor I, Eosinophil differentiation factor, T-cell replacing factor, TRF, IL5
Recommended products
Anti-IL-5 [EPR20012-256] (Capture) antibody (ab242955) is a rabbit monoclonal antibody that is provided in a PBS only formulation free from BSA and azide. Formulated to be conjugation-ready. It is used to detect IL-5 in ELISA. Suitable for Human samples.
- BSA, sodium azide, and glycerol-free for consistent conjugation with fluorochromes, enzymes and more
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR20012-256
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (ab242955) is a rabbit recombinant monoclonal antibody and is validated for use in ELISA in Human samples.
Other related products
We have a range of other formats of antibody clone [EPR20012-256] also available for your convenience:
Anti-IL-4R antibody [EPR28312-39] ab320080, Carrier free - Anti-IL-4R antibody [EPR28312-39] - BSA and Azide free ab320081, Carrier free - ab242955
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (ab242955), expandable thumbnail](https://content.abcam.com/products/images/il-5-antibody-epr20012-256-bsa-and-azide-free-capture-ab242955--sandwich-elisa-img181940.jpg "Sandwich ELISA - Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (ab242955)")
Sandwich ELISA - Anti-IL-5 antibody [EPR20012-256] - BSA and Azide free (Capture) (ab242955)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human IL-5 ELISA Kit ab215536).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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