Rabbit Recombinant Monoclonal IL-5RA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-IL-5RA antibody [EPR23848-508] - BSA and Azide free (Detector) (AB280999), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr23848-508-bsa-and-azide-free-detector-ab280999--sandwich-elisa-img174744.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-5RA antibody [EPR23848-508] - BSA and Azide free (Detector) (AB280999)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
This is the receptor for interleukin-5. The alpha chain binds to IL5.
Alternative names
Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, IL5R, IL5RA
Recommended products
Rabbit Recombinant Monoclonal IL-5RA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Alternative names
Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125, IL5R, IL5RA
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EPR23848-508
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
ab280999 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL5RA. The recommended pair for sandwich ELISA is:
Capture: Anti-IL-5RA antibody [EPR23848-441] - BSA and Azide free (Capture) ab281149, Human IL5RA Capture Antibody (unconjugated)
Detector: ab280999, Human IL5RA Detector Antibody (unconjugated)
The reference range value is 20 - 1250 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-5RA also known as the interleukin-5 receptor alpha chain or IL-5 receptor is a protein with a critical role in the immune system. It is also referred to as CD125 or CaL40. The protein has an approximate mass of 52 kDa and is expressed mainly on eosinophils and basophils which are types of white blood cells involved in immune responses. The IL-5RA forms a part of the IL-5 receptor complex which also includes a beta chain responsible for signal transduction.
- Biological function summary
IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.
- Pathways
IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.
- Associated diseases and disorders
IL-5RA is linked with asthma and eosinophilic esophagitis conditions characterized by an abnormal proliferation and activation of eosinophils. In asthma for example the increased activity of IL-5RA leads to elevated levels of eosinophils contributing to airway inflammation and hyperresponsiveness. IL-5RA also connects with other proteins like IL-5 itself serving as a therapeutic target in treatments aimed at regulating excessive eosinophil activity in these disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-IL-5RA antibody [EPR23848-508] - BSA and Azide free (Detector) (ab280999), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr23848-508-bsa-and-azide-free-detector-ab280999--sandwich-elisa-img174744.jpg "Sandwich ELISA - Anti-IL-5RA antibody [EPR23848-508] - BSA and Azide free (Detector) (ab280999)")
Sandwich ELISA - Anti-IL-5RA antibody [EPR23848-508] - BSA and Azide free (Detector) (ab280999)
Example of Human IL-5 Receptor alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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