Rabbit Recombinant Monoclonal IL-5RA antibody. Suitable for Flow Cyt and reacts with Human, Mouse samples.
Images
![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445889.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)")
![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445888.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)")
![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445887.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (AB324011)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | IHC-P | WB | |
|---|---|---|---|
| Human | Tested | Not recommended | Not recommended |
| Mouse | Tested | Not recommended | Not recommended |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 | Notes - |
Species Mouse | Dilution info 1/50 | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed:1495999, PubMed:22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
Alternative names
CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125
Rabbit Recombinant Monoclonal IL-5RA antibody. Suitable for Flow Cyt and reacts with Human, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR28313-257
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445889.jpg "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)")
Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)
IL-5RA Flow Cytometry staining of Fresh mouse blood cells using rabbit Anti-IL-5RA antibody
Flow cytometric analysis of Fresh mouse blood cells labelling IL-5RA with ab324011 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Fresh mouse blood cells were co-stained with CD3 conjugated to Alexa Fluor®647.
Gated on viable cells.![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445888.jpg "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)")
Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)
IL-5RA Flow Cytometry staining of Fresh mouse blood cells using rabbit Anti-IL-5RA antibody
Flow cytometric analysis of Fresh mouse blood cells labelling IL-5RA with ab324011 at 1/50 dilution (1 ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Fresh mouse blood cells were co-stained with CD11b conjugated to Brilliant Violet 421.
Gated on viable granulocyte population(SSC-A high).![Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011), expandable thumbnail](https://content.abcam.com/products/images/il-5ra-antibody-epr28313-257-ab324011--flow-cytometry-img445887.jpg "Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)")
Flow Cytometry - Anti-IL-5RA antibody [EPR28313-257] (ab324011)
IL-5RA Flow Cytometry staining of Human purified neutrophils using rabbit Anti-IL-5RA antibody
Flow cytometric analysis of Human purified neutrophils cells labelling IL-5RA with ab324011 at 1/500 dilution (0.1 ug) (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
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