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AB134935

Anti-IL-5RA antibody [EPR5450(2)]

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(2 Publications)

Rabbit Recombinant Monoclonal IL-5RA antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

CD125, IL5R, IL5RA, Interleukin-5 receptor subunit alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5RA, CDw125

1 Images
Western blot - Anti-IL-5RA antibody [EPR5450(2)] (AB134935)
  • WB

Unknown

Western blot - Anti-IL-5RA antibody [EPR5450(2)] (AB134935)

All lanes:

Western blot - Anti-IL-5RA antibody [EPR5450(2)] (ab134935) at 1/1000 dilution

Lane 1:

Human platelet lysate at 10 µg

Lane 2:

Human serum lysate at 10 µg

Secondary

All lanes:

Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 48 kDa

false

  • Carrier free

    Anti-IL-5RA antibody [EPR5450(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5450(2)

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-5RA also known as the interleukin-5 receptor alpha chain or IL-5 receptor is a protein with a critical role in the immune system. It is also referred to as CD125 or CaL40. The protein has an approximate mass of 52 kDa and is expressed mainly on eosinophils and basophils which are types of white blood cells involved in immune responses. The IL-5RA forms a part of the IL-5 receptor complex which also includes a beta chain responsible for signal transduction.
Biological function summary

IL-5RA plays an important role in the growth differentiation and activation of eosinophils. The protein forms part of the IL-5 receptor complex which binds to the cytokine IL-5 initiating a signaling cascade that results in these processes. Through its interaction with IL-5 IL-5RA influences the survival and function of eosinophils cells critical for responses against parasitic infections and in allergic reactions.

Pathways

IL-5RA is fundamental in the JAK-STAT signaling pathway. This pathway is significant as it facilitates the transduction of extracellular cytokine signals into cellular responses impacting the growth and activity of eosinophils. The IL-5 receptor complex is associated with other cytokine receptors through this pathway involving proteins such as JAK2 and STAT5 which play essential roles in the activation of downstream signaling events and cellular responses.

IL-5RA is linked with asthma and eosinophilic esophagitis conditions characterized by an abnormal proliferation and activation of eosinophils. In asthma for example the increased activity of IL-5RA leads to elevated levels of eosinophils contributing to airway inflammation and hyperresponsiveness. IL-5RA also connects with other proteins like IL-5 itself serving as a therapeutic target in treatments aimed at regulating excessive eosinophil activity in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed : 9378992). Acts by forming a heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed : 1495999, PubMed : 22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed : 9516124).
See full target information IL5RA
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death discovery 8:227 PubMed35468881

2022

JMJD2C mediates the MDM2/p53/IL5RA axis to promote CDDP resistance in uveal melanoma.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhu,Han Chen,Xiaoying Li,Xi Wang,Hongtao Yan

Journal of science, technology and environment 5: PubMed26770888

2016

Increase in Blood Glutathione and Erythrocyte Proteins Related to Glutathione Generation, Reduction and Utilization in African-American Old Women with Diabetes.

Applications

Unspecified application

Species

Unspecified reactive species

Guang Shan,Fang Yang,LiChun Zhou,Tian Tang,Emmanuel U Okoro,Hong Yang,ZhongMao Guo
View all publications
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