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Anti-IL-6 antibody [1.2-2B11-2G10] is a mouse monoclonal antibody that is used to detect IL-6 in IHC-P, Western blot, sELISA. Suitable for Human, Rat samples.

- Cited in over 320 publications
- Antibody clone 1.2-2B11-2G10 has been tried and trusted by researchers since 2002 and is cited in >390 publications


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (AB9324), expandable thumbnail
  • Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (AB9324), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (AB9324), expandable thumbnail
  • Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (AB9324), expandable thumbnail

Publications

Key facts

Isotype
IgG2a
Host species
Mouse
Storage buffer
Standard buffer
Form
Lyophilized
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human IL6. Database link P05231

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWBsELISA
Human
Tested
Expected
Expected
Rat
Expected
Tested
Expected
Recombinant full length protein - Human
Not recommended
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
1 µg/mL
Notes

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Rat
Dilution info
-
Notes

To detect human IL-6 by Western blot analysis this antibody can be used at a concentration of 0.50-1.0 μg/mL. When used in conjunction with compatible secondary reagents the detection limit for recombinant human IL-6 is 1.0-2.0 ng/lane, under reducing conditions and non-reducing conditions.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Recombinant full length protein - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Recombinant full length protein - Human
Dilution info
1.00000-2.00000 mg/mL
Notes

In a sandwich ELISA (assuming 100μl/well), a concentration of 1.0-2.0 μg/ml of this antibody will detect at least 1000pg/ml of Recombinant Human IL-6 when used with Biotin Anti-IL-6 antibody ab271236 detection antibody at a concentration of approximately 0.10-0.20 μg/ml.

Expected
Expected

Species
Human, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability (PubMed:12794819, PubMed:17075861). Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed:12794819). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed:20823453). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed:15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

Alternative names

Recommended products

Anti-IL-6 antibody [1.2-2B11-2G10] is a mouse monoclonal antibody that is used to detect IL-6 in IHC-P, Western blot, sELISA. Suitable for Human, Rat samples.

- Cited in over 320 publications
- Antibody clone 1.2-2B11-2G10 has been tried and trusted by researchers since 2002 and is cited in >390 publications

Key facts

Isotype
IgG2a
Form
Lyophilized
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human IL6. Database link P05231
Clone number
1.2-2B11-2G10
Purification technique
Affinity purification Protein A
Reconstitution
Reconstitute with 500µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Notes

Product Specifications

Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) is a mouse monoclonal antibody and is validated for use in IHC-P, WB, sELISA in human, rat samples.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) specifically detects IL-6 (UniProt ID: P05231; Molecular weight: 21kDa) and is sold in 500 µg selling sizes.

Quality and Validation

Abcam's high quality validation processes ensure Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has high sensitivity and specificity.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has been cited over 320 times in peer reviewed journals and is trusted by the scientific community.
Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) has 20 independent reviews from customers.

Although some customers have been successful in IHC we no longer batch test in this application.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

Associated diseases and disorders

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

Product promise

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4 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tumor tissue labelling IL-6 with ab9324 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324), expandable thumbnail

    Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

    IL-6 Western blot staining using mouse Anti-IL-6 antibody

    This antibody was raised against an immunogen that is predicted to recognize the glycosylated form of IL6 as well as the IL6delta4 splice variant. The predicted molecular weights are 25 kDa and 17 kDa respectively. The band observed at 50 kDa may represent multimers of IL6 as reported in the literature.

    All lanes: Western blot - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324) at 1/2000 dilution

    Lane 1: Human Spleen at 20 µg

    Lane 2: Human Lung at 20 µg

    Lane 3: Rat Spleen

    Lane 4: Rat Lung at 20 µg

    Secondary

    All lanes: 800CW Goat Anti-Mouse IgG at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 23 kDa

    Observed band size: 17 kDa, 25 kDa, 50 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tumor tissue labelling IL-6 with ab9324 at 1 μg/mL (45 minute incubation at room temperature). Heat mediated antigen retrieval was performed using a buffer at pH 6. An HRP-labeled polymer detection system was used with a DAB chromogen.

  • Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324), expandable thumbnail

    Sandwich ELISA - Anti-IL-6 antibody [1.2-2B11-2G10] (ab9324)

    Assuming 100 µL/well, a concentration of 1.0-2.0 µg/mL of ab9324 will detect at least 1,000 pg/mL of Recombinant Human IL-6 when used with Biotin Anti-IL-6 antibody (Biotin Anti-IL-6 antibody ab271236) as the detection antibody at a concentration of approximately 0.10-0.20 µg/mL.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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