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Rabbit Polyclonal IL-6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 624 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL6.

Isotype

IgG

Host species

Rabbit

Storage buffer

Constituents: 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

Recombinant Full Length Protein corresponding to Human IL6. Database link: P05231

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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-P
Human
Tested
Tested
Pig
Predicted
Predicted

Images

Tested
Tested

Species
Human
Dilution info
1/500 - 1/2000
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/400 - 1/800
Notes

-

Predicted
Predicted

Species
Pig
Dilution info
-
Notes

-

Target data

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Rabbit Polyclonal IL-6 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 624 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human IL6.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

Recombinant Full Length Protein corresponding to Human IL6. Database link: P05231

Purity

Whole antiserum

Specificity

ab6672 detects a band at 25 kDa in human lung tissue lysate and mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6.
Please be aware that this product has low homology with the mouse and rat sequence of IL6 (Rat, 40%; Mouse 41%, UniProt blast) and we therefore cannot guarantee reactivity in these species.

Concentration
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Purification notes

Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts).

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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