Anti-IL-6 antibody is a rabbit polyclonal antibody that is used to detect IL-6 in IHC-P, Western blot. Suitable for Human samples.
- Cited in over 600 publications
- Tried and trusted by researchers since 2001
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- International journal of molecular sciences 24:2023Olanzapine Effects on Parvalbumin/GAD67 Cell Numbers in Layers/Subregions of Dorsal Hippocampus of Chronically Socially Isolated Rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrijana Stanisavljević Ilić,Snežana Đorđević,Dragoš Inta,Stefan Borgwardt,Dragana FilipovićPubMed 38139008
- Cells 12:2023The Proinflammatory Role of ANGPTL8 R59W Variant in Modulating Inflammation through NF-κB Signaling Pathway under TNFα Stimulation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMohamed Abu-Farha,Dhanya Madhu,Prashantha Hebbar,Anwar Mohammad,Arshad Channanath,Sina Kavalakatt,Nada Alam-Eldin,Fatima Alterki,Ibrahim Taher,Osama Alsmadi,Mohammad Shehab,Hossein Arefanian,Rasheed Ahmad,Thangavel Alphonse Thanaraj,Fahd Al-Mulla,Jehad AbubakerPubMed 37947641
- International journal of inflammation 2023:38030562023The PTP1B Inhibitor Trodusquemine (MSI-1436) Improves Glucose Uptake in Equine Metabolic Syndrome Affected Liver through Anti-Inflammatory and Antifibrotic Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLynda Bourebaba,Anna Serwotka-Suszczak,Nabila Bourebaba,Magdalena Zyzak,Krzysztof MaryczPubMed 37808009
- Animal nutrition (Zhongguo xu mu shou yi xue hui) 15:159-1722023Piglet growth performance improved by dietary supplementation of porous or nano particles of zinc oxide may be related to the gut microbiota.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLina Long,Xichen Zhao,Jie Chen,Zixi Wang,Yanfang Tang,Jian Huang,Yulong YinPubMed 38023375
- The Journal of clinical investigation 133:2023Pancreatic RECK inactivation promotes cancer formation, epithelial-mesenchymal transition, and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomonori Masuda,Akihisa Fukuda,Go Yamakawa,Mayuki Omatsu,Mio Namikawa,Makoto Sono,Yuichi Fukunaga,Munemasa Nagao,Osamu Araki,Takaaki Yoshikawa,Satoshi Ogawa,Kenji Masuo,Norihiro Goto,Yukiko Hiramatsu,Yu Muta,Motoyuki Tsuda,Takahisa Maruno,Yuki Nakanishi,Toshihiko Masui,Etsuro Hatano,Tomoko Matsuzaki,Makoto Noda,Hiroshi SenoPubMed 37712427
- FASEB journal : official publication of the Federation of American Societies for Experimental Biology 37:e231672023Differential outcomes of high-fat diet on age-related rescaling of cochlear frequency place coding.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu Zhang,Guotong Lin,Na Xue,Yi Wang,Tingting Du,Huihui Liu,Wei Xiong,Wei Shang,Hao Wu,Lei SongPubMed 37651093
- International journal of molecular sciences 24:2023Prenatal High-Fat Diet Combined with Microplastic Exposure Induces Liver Injury via Oxidative Stress in Male Pups.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMao-Meng Tiao,Jiunn-Ming Sheen,I-Chun Lin,Madalitso Khwepeya,Hong-Ren YuPubMed 37686267
- Journal of periodontal research 58:1061-10812023Proteomic profile of human gingival crevicular fluid reveals specific biological and molecular processes during clinical progression of periodontitis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAlfredo Torres,M Angélica Michea,Ákos Végvári,Marion Arce,Alicia Morales,Elías Lanyon,Marcela Alcota,Camila Fuentes,Rolando Vernal,Mauricio Budini,Roman A Zubarev,Fermín E GonzálezPubMed 37522282
- Cancers 15:2023Co-Expression of Immunohistochemical Markers MRP2, CXCR4, and PD-L1 in Gallbladder Tumors Is Associated with Prolonged Patient Survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndrés Tittarelli,Omar Barría,Evy Sanders,Anna Bergqvist,Daniel Uribe Brange,Mabel Vidal,María Alejandra Gleisner,Jorge Ramón Vergara,Ignacio Niechi,Iván Flores,Cristián Pereda,Cristian Carrasco,Claudia Quezada-Monrás,Flavio Salazar-OnfrayPubMed 37444550
- Intensive care medicine experimental 11:352023Colonic oxygen microbubbles augment systemic oxygenation and CO removal in a porcine smoke inhalation model of severe hypoxia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaul A Mountford,Premila D Leiphrakpam,Hannah R Weber,Andrea McCain,Robert M Scribner,Robert T Scribner,Ernesto M Duarte,Jie Chen,Dragana Noe,Mark A Borden,Keely L BuesingPubMed 37357222
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human IL6. Database link P05231
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Pig | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/400 - 1/800 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Pig | Dilution info - | Notes - |
Associated Products
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Target data
Function
Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability (PubMed:12794819, PubMed:17075861). Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed:12794819). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed:20823453). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed:15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).
Alternative names
IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2
Recommended products
Anti-IL-6 antibody is a rabbit polyclonal antibody that is used to detect IL-6 in IHC-P, Western blot. Suitable for Human samples.
- Cited in over 600 publications
- Tried and trusted by researchers since 2001
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Constituents: 98.6% Whole serum, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human IL6. Database link P05231
- Purity
- Whole antiserum
- Specificity
ab6672 detects a band at 25 kDa in human lung tissue lysate and mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6. Please be aware that this product has low homology with the mouse and rat sequence of IL6 (Rat, 40%; Mouse 41%, UniProt blast) and we therefore cannot guarantee reactivity in these species.
- Concentration
- Purification notes
Anti-IL-6 antiserum detects recombinant and native IL-6 present in body fluids and cell supernatants in various assays (ie. IL-1 stimulated IL-6 production from fibroblasts).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Product Specifications
Anti-IL-6 antibody (ab6672) is a rabbit polyclonal antibody and is validated for use in IHC-P, WB in human samples.
Anti-IL-6 antibody (ab6672) specifically detects IL-6 (UniProt ID: P05231; Molecular weight: 21kDa) and is sold in 200 µL selling sizes.
Quality and Validation
Abcam's high quality validation processes ensure Anti-IL-6 antibody (ab6672) has high sensitivity and specificity.
Anti-IL-6 antibody (ab6672) has been cited over 625 times in peer reviewed journals and is trusted by the scientific community.
Anti-IL-6 antibody (ab6672) has 43 independent reviews from customers.
IL-6 synonyms: plasmacytoma growth factor (PCT-GF),interferon-a-2 (IFN-a2), monocyte derived human B cellgrowth factor, B cell stimulating factor (BSF-2),hepatocyte stimulating factor (HSF), and interleukinhybridoma/plasmacytoma-1 (IL-HP1).
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.
- Biological function summary
IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.
- Pathways
IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.
- Associated diseases and disorders
IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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Lee EJ et al. Radiation Inhibits Interleukin-12 Production via Inhibition of C-Rel through the Interleukin-6/ Signal Transducer and Activator of Transcription 3 Signaling Pathway in Dendritic Cells. PLoS One 11:e0146463 (2016).Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
IL6 (Red) and IL-12 (Green) were measured at 1, 3, and 7 days after 10 Gy irradiation of HCa-1 tumors to determine whether irradiation regulates IL-12 and IL6 expression in tumours. ab6672 was used to stain IL6 at 1/100 dilution in immunohistochemical analysis.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6 antibody (ab6672)
IHC image of IL6 staining in human lung formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab6672, 1/400, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Anti-IL-6 antibody (ab6672)
Tissue lysates were denatured for 10-15 minutes at 90°C. ab6672 was incubated overnight at 4°C and the secondary antibody for 1 hour at RT.
ab6672 detects a band at 25 kDa in Human lung tissue lysate and Mouse spleen tissue lysate, however the signal in mouse tissue is significantly lower. It also binds strongly to a protein at ~55 kDa in Human lung tissue extracts, which we believe represents a glycosylated form of IL6. ab6672 also detects several bands in Human lung tissue lysate within the region of 30-40 kDa. These may represent heteromers of IL6.
All lanes: Western blot - Anti-IL-6 antibody (ab6672) at 1/500 dilution
Lane 1: Human spleen tissue lysate at 20 µg
Lane 2: Human lung tissue lysate at 20 µg
Lane 3: Mouse spleen tissue lysate at 20 µg
Lane 4: Mouse lung tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 23 kDa
Observed band size: 25 kDa, 55 kDa
Western blot - Anti-IL-6 antibody (ab6672)
4-20% Tris-Glycine gel.
The membrane was blocked for 30 minutes with 1% BSA-TBST.
All lanes: Western blot - Anti-IL-6 antibody (ab6672) at 1/500 dilution
All lanes: recombinant human IL-6
SecondaryAll lanes: conjugated anti-Rabbit IgG at 1/40000 dilution
Developed using the ECL technique.
Predicted band size: 23 kDa
Observed band size: 21 kDa
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