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Rabbit Recombinant Monoclonal IL-6 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 37 publications.

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Images

Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (AB259341), expandable thumbnail
  • Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (AB259341), expandable thumbnail
  • Western blot - Anti-IL-6 antibody [EPR23819-11] (AB259341), expandable thumbnail
  • Western blot - Anti-IL-6 antibody [EPR23819-11] (AB259341), expandable thumbnail
  • Western blot - Anti-IL-6 antibody [EPR23819-11] (AB259341), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-FrFlow Cyt (Intra)IHC-P
Mouse
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Tested
Tested
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Species
Rat
Dilution info
1/30
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Cytokine with a wide variety of biological functions in immunity, tissue regeneration, and metabolism. Binds to IL6R, then the complex associates to the signaling subunit IL6ST/gp130 to trigger the intracellular IL6-signaling pathway (Probable). The interaction with the membrane-bound IL6R and IL6ST stimulates 'classic signaling', whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable). IL6 is a potent inducer of the acute phase response. Rapid production of IL6 contributes to host defense during infection and tissue injury, but excessive IL6 synthesis is involved in disease pathology. In the innate immune response, is synthesized by myeloid cells, such as macrophages and dendritic cells, upon recognition of pathogens through toll-like receptors (TLRs) at the site of infection or tissue injury (Probable). In the adaptive immune response, is required for the differentiation of B cells into immunoglobulin-secreting cells. Plays a major role in the differentiation of CD4(+) T cell subsets. Essential factor for the development of T follicular helper (Tfh) cells that are required for the induction of germinal-center formation. Required to drive naive CD4(+) T cells to the Th17 lineage. Also required for proliferation of myeloma cells and the survival of plasmablast cells (By similarity). Acts as an essential factor in bone homeostasis and on vessels directly or indirectly by induction of VEGF, resulting in increased angiogenesis activity and vascular permeability (PubMed:12794819, PubMed:17075861). Induces, through 'trans-signaling' and synergistically with IL1B and TNF, the production of VEGF (PubMed:12794819). Involved in metabolic controls, is discharged into the bloodstream after muscle contraction increasing lipolysis and improving insulin resistance (PubMed:20823453). 'Trans-signaling' in central nervous system also regulates energy and glucose homeostasis (By similarity). Mediates, through GLP-1, crosstalk between insulin-sensitive tissues, intestinal L cells and pancreatic islets to adapt to changes in insulin demand (By similarity). Also acts as a myokine (Probable). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). Also has a pivotal role in iron metabolism by regulating HAMP/hepcidin expression upon inflammation or bacterial infection (PubMed:15124018). Through activation of IL6ST-YAP-NOTCH pathway, induces inflammation-induced epithelial regeneration (By similarity).

Alternative names

IFNB2, IL6, Interleukin-6, IL-6, B-cell stimulatory factor 2, CTL differentiation factor, Hybridoma growth factor, Interferon beta-2, BSF-2, CDF, IFN-beta-2

Recommended products

Rabbit Recombinant Monoclonal IL-6 antibody. Suitable for IP, WB and reacts with Mouse, Rat samples. Cited in 37 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23819-11
Purification technique
Affinity purification Protein A
Specificity

FURTHER INFORMATION ON SPECIFICITY (Chinese Version) available under the support & downloads section.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Interleukin-6 (IL-6) a cytokine also known as IFN-beta2 plays a significant role in immune response and inflammation. The IL-6 protein has a molecular weight of approximately 20-26 kDa. Expression of IL-6 occurs in various cell types including T cells macrophages and fibroblasts. Researchers often measure IL-6 levels in biological samples using IL-6 ELISA kits an essential tool for studying this protein’s function and presence in experimental and clinical settings.

Biological function summary

IL-6 influences immune regulation and acts as part of the acute phase response. It stimulates the production of acute-phase proteins and supports the differentiation of B cells into antibody-producing cells. IL-6 is not known to be part of a larger complex acting primarily as a single entity in signal transduction. Moreover IL-6 impacts the metabolism of iron and bone homeostasis showing its multifunctional nature.

Pathways

IL-6 forms an integral part of several signaling routes particularly the JAK-STAT pathway. In this context IL-6 interacts with signal transducer proteins like STAT3 to transmit signals from the cell surface to the nucleus affecting gene expression. Another important pathway is the MAPK pathway through which IL-6 influences cell proliferation and survival. These interactions reflect IL-6's diverse effects in cellular processes.

Associated diseases and disorders

IL-6's association with rheumatoid arthritis and multiple myeloma emphasizes its role in chronic inflammation and cancer. In rheumatoid arthritis IL-6 contributes to inflammation and joint damage often together with TNF-alpha highlighting a potential target for anti-inflammatory therapies. In multiple myeloma IL-6 supports the survival and proliferation of cancerous plasma cells highlighting its importance in cancer progression and possible treatment targets.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341), expandable thumbnail

    Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    IL-6 was immunoprecipitated from 0.35 mg RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 0.1 μg/ml lipopolysaccharide (LPS) for 4 hours, then with 1 ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate 10 ug with ab259341 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259341 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 0.1 ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate 10 ug

    Lane 2: ab259341 IP in RAW264.7 treated with 0.1ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate

    Lane 3:Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259341 in RAW264.7 treated with 0.1ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    Predicted band size: 23 kDa

    Observed band size: 24 kDa

  • Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341), expandable thumbnail

    Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    IL-6 was immunoprecipitated from 0.35 mg NR8383 (rat lung macrophage (alveolar)) treated with 0.1 ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1 ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate 10 ug with ab259341 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab259341 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NR8383 (rat lung macrophage (alveolar)) treated with 0.1 ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1 ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate 10 ug

    Lane 2: ab259341 IP in NR8383 treated with 0.1ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1 ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab259341 in NR8383 treated with 0.1ug/ml lipopolysaccharide (LPS) for 4 hours, then with 1 ug/ml Brefeldin A (BFA) added for 3 hours whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes

    All lanes: Immunoprecipitation - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    Predicted band size: 23 kDa

    Observed band size: 24 kDa

  • Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341), expandable thumbnail

    Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST. This blot was developed using a higher sensitivity ECL substrate.

    The molecular weight is consistent with what has been described in the literatures (PMID:2523818, PMID:25130514)

    Exposure time: 3 minutes

    All lanes: Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341) at 1/1000 dilution

    Lane 1: Untreated NR8383 (rat lung macrophage (alveolar)) whole cell lysate at 20 µg

    Lane 2: NR8383 (rat lung macrophage (alveolar)) treated with 0.1 µg/ml lipopolysaccharide (LPS) for 4 hours, then with 1 μg/ml Brefeldin A (BFA) added for 3 hours whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 23 kDa

    Observed band size: 20 kDa

  • Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341), expandable thumbnail

    Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    Normal tissues express undetectable level of IL-6 protein.

    All lanes: Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341) at 1/1000 dilution

    Lane 1: Mouse intestine tissue lysate at 20 µg

    Lane 2: Mouse intestine tissue treated with 1ug/ml LPS and 1ug/ml Brefeldin A (BFA) for 16 hours whole cell lysate at 20 µg

    Lane 3: Mouse spleen tissue lysate at 20 µg

    Lane 4: Mouse colon tissue lysate at 20 µg

    Lane 5: Mouse liver tissue lysate at 20 µg

    Lane 6: Mouse brain tissue lysate at 20 µg

    Lane 7: Mouse hypothalamus tissue lysate at 20 µg

    Lane 8: Mouse thymus tissue lysate at 20 µg

    Lane 9: Mouse bladder tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Exposure time: 140s

  • Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341), expandable thumbnail

    Western blot - Anti-IL-6 antibody [EPR23819-11] (ab259341)

    Normal tissues express undetectable level of TNF alpha, IL-1 beta and IL-6 proteins.
    IL-1 beta and TNF alpha could be good controls validating the increased IL-6 level after drug treatment.

    Lanes 1 - 9: Anti-TNF alpha antibody [EPR21753-109] ab205587 1:1000 dilution (0.6 ug/ml) and Anti-IL-1 beta antibody [EPR23851-127] ab254360 1:1000 dilution (0.5 ug/ml) at 1/1000 dilution

    Lanes 1 - 9: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)

    Lane 1: Rat cerebral cortex tissue lysate at 20 µg

    Lane 2: Rat cerebellum tissue lysate at 20 µg

    Lane 3: Rat hippocampus tissue lysate at 20 µg

    Lane 4: Rat spinal cord tissue lysate at 20 µg

    Lane 5: Rat small intestine tissue lysate at 20 µg

    Lane 6: Rat kidney tissue lysate at 20 µg

    Lane 7: Rat skin tissue lysate at 20 µg

    Lane 8: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 20 µg

    Lane 9: RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) treated with 100ng/ml lipopolysaccharide (LPS) for 4h then add 1000ng/ml BFA for another 3h whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 20 kDa

    Exposure time: 180s

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