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AB323483

Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin

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Rabbit Recombinant Monoclonal IL-6R antibody. Carrier free. Suitable for FuncS (Neut/Block) and reacts with Human samples.

View Alternative Names

CD126, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1, Membrane glycoprotein 80, gp80, IL6R

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Functional Studies (Neut/Block) - Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin (AB323483)
  • FuncS (Neut/Block)

Supplier Data

Functional Studies (Neut/Block) - Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin (AB323483)

Comparison of ab323372 (A) and a leading competitor’s antibody (B) in a neutralization assay assessing IL-6-dependent cell proliferation in human TF-1 cells. The competitor’s antibody showed better inhibition of TF-1 cells proliferation with lower IC50.

TF-1 cells were plated at a density of 5 × 10⁴ cells per well in a 96-well plate and cultured in medium containing 5% FBS. A mixture of IL-6 (ab259381) and a dilution series of anti-IL-6 antibody (ab323372 or leading competitor's antibody) was added to the cells, followed by incubation for 48 h. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.

Functional Studies (Neut/Block) - Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin (AB323483)
  • FuncS (Neut/Block)

Supplier Data

Functional Studies (Neut/Block) - Anti-IL-6R alpha antibody [EPR21714-182R] - BSA and Azide free, low endotoxin (AB323483)

Cell proliferation of TF-1 (human erythroleukemia erythroblast) cells treated with IL-6 (ab259381) and anti-IL-6R antibody (ab323483) or isotype IgG (ab210849).

Cell proliferation (%) (A) confirms that the anti-IL-6R antibody (ab323483) neutralizes IL-6-mediated cell proliferation. The IC50 as determined by the dose-dependent inhibition of TF-1 cells proliferation is 0.1914 ug/ml (B).

TF-1 cells were plated at a density of 5 × 10⁴ cells per well in a 96-well plate. A dilution series of the anti-IL-6R antibody (ab323483) was added to the cells and pre-incubated for 2 hours before IL-6 (ab259381) stimulation, and cells were cultured in medium with 5% FBS for a duration of 48 hours. Proliferation was analysed by luminescent assay and recorded using a Microplate Reader at 560nm emission.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR21714-182R

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

FuncS (Neut/Block)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

Assay Kits

AB278117

Human IL-6 R alpha ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-6 R alpha ELISA Kit (AB278117)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FuncSNeutBlock" : {"fullname" : "Functional Studies (Neut/Block)", "shortname":"FuncS (Neut/Block)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FuncSNeutBlock-species-checked": "testedAndGuaranteed", "FuncSNeutBlock-species-dilution-info": "", "FuncSNeutBlock-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal (PubMed : 28265003). Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis (PubMed : 30995492, PubMed : 31235509). The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6 : IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells (Probable).. Isoform 1. Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (By similarity).. Isoform 2. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 21990364). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed : 21990364). In complex with IL6, is required for induction of VEGF production (PubMed : 12794819). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).. Soluble interleukin-6 receptor subunit alpha. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed : 21990364). The IL6 : sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (PubMed : 21990364). In complex with IL6, is required for induction of VEGF production (PubMed : 12794819). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (By similarity). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (By similarity).
See full target information IL6R
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Product promise

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