Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free) (AB300582)

This data was developed using ab300581, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse thymus tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse thymus is observed. The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead of primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free) (AB300582)

This data was developed using ab300581, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling Interleukin-6 receptor subunit alpha with ab300581 at 1/500 dilution (5.04 µg/mL) followed by a ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on mouse spleen is observed (PMID : 23378927). The section was incubated with ab300581 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : PBS was used instead od primary antibody, followed by a ready to use secondary antibody Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins was used.

• WB

Supplier Data

Western blot - Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free) (AB300582)

This data was developed using ab300581, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST.  Low expression : WEHI-231(PMID : 2112585) The identity of the lower MW band at approximately 40 kDa (in lane 1) is unknown.

All lanes:

Western blot - Anti-IL-6R antibody [EPR26370-132] (<a href='/en-us/products/primary-antibodies/il-6r-antibody-epr26370-132-ab300581'>ab300581</a>) at 1/1000 dilution

Lane 1:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate

Lane 2:

WEHI-231 (mouse B cell lymphoma B lymphocyte), whole cell lysate

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 70-80 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-IL-6R antibody [EPR26370-132] (BSA and Azide free) (AB300582)

This data was developed using ab300581, the same antibody clone in a different buffer formulation.  Blocking and diluting buffer and concentration : 5% NFDM/TBST.  Target is a glycoprotein of approximately 75 kDa and detected as a 55kDa band after treated with Protein Deglycosylation MIX II.  Exposure time : 180 seconds.

All lanes:

Western blot - Anti-IL-6R antibody [EPR26370-132] (<a href='/en-us/products/primary-antibodies/il-6r-antibody-epr26370-132-ab300581'>ab300581</a>) at 1/1000 dilution

Lane 1:

Untreated mouse spleen tissue lysate,

Lane 2:

Mouse spleen tissue lysate treated with Protein Deglycosylation Mix II,

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 55 kDa,70 kDa

false

Exposure time: 180s