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Rabbit Recombinant Multiclonal IL-6R antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human, Mouse samples.

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Images

Flow Cytometry - Anti-IL-6R antibody [RM2071] (AB322899), expandable thumbnail
  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (AB322899), expandable thumbnail
  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (AB322899), expandable thumbnail
  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (AB322899), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-6R antibody [RM2071] (AB322899), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFFlow CytIPIHC-PWB
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Species
Mouse
Dilution info
1/50
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Species
Mouse
Dilution info
1/498
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Human
Dilution info
-
Notes

-

Target data

Function

Part of the receptor for interleukin 6. Binds to IL6 with low affinity, but does not transduce a signal. Signal activation necessitate an association with IL6ST. Activation leads to the regulation of the immune response, acute-phase reactions and hematopoiesis. The interaction with membrane-bound IL6R and IL6ST stimulates 'classic signaling', the restricted expression of the IL6R limits classic IL6 signaling to only a few tissues such as the liver and some cells of the immune system. Whereas the binding of IL6 and soluble IL6R to IL6ST stimulates 'trans-signaling'. Alternatively, 'cluster signaling' occurs when membrane-bound IL6:IL6R complexes on transmitter cells activate IL6ST receptors on neighboring receiver cells. Interleukin-6 receptor subunit alpha. Signaling via the membrane-bound IL6R is mostly regenerative and anti-inflammatory (Probable). Drives naive CD4(+) T cells to the Th17 lineage, through 'cluster signaling' by dendritic cells (PubMed:27893700). Soluble interleukin-6 receptor subunit alpha. Soluble form of IL6 receptor (sIL6R) that acts as an agonist of IL6 activity (PubMed:11113088). The IL6:sIL6R complex (hyper-IL6) binds to IL6ST/gp130 on cell surfaces and induces signaling also on cells that do not express membrane-bound IL6R in a process called IL6 'trans-signaling'. sIL6R is causative for the pro-inflammatory properties of IL6 and an important player in the development of chronic inflammatory diseases (By similarity). In complex with IL6, is required for induction of VEGF production (By similarity). Plays a protective role during liver injury, being required for maintenance of tissue regeneration (PubMed:11113088). 'Trans-signaling' in central nervous system regulates energy and glucose homeostasis (PubMed:28402851).

Additional Targets

IL6R

Alternative names

CD126, Il6r, Il6ra, Interleukin-6 receptor subunit alpha, IL-6 receptor subunit alpha, IL-6R subunit alpha, IL-6R-alpha, IL-6RA, IL-6R 1

Rabbit Recombinant Multiclonal IL-6R antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human, Mouse samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Multiclonal
Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
RM2071
Purification technique
Affinity purification Protein A
Specificity

Unsuitable for mouse WB.

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899)

    Flow cytometric analysis of Mouse PBMC cells labelling IL-6R with ab322899 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    mouse PBMC are co-stained with CD19 conjugated PE/Cy7.

    Gated on viable cell.

  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899)

    Flow cytometric analysis of Mouse PBMC cells labelling IL-6R with ab322899 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    mouse PBMC are co-stained with CD3 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899)

    Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling IL-6R with ab322899 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    human PBMC are co-stained with CD19 conjugated PE/Cy7.

    Gated on viable cell.

  • Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Flow Cytometry - Anti-IL-6R antibody [RM2071] (ab322899)

    Flow cytometric analysis of Human PBMC(human peripheral blood mononuclear cell) cells labelling IL-6R with ab322899 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    human PBMC are co-stained with CD3 conjugated Alexa Fluor®647.

    Gated on viable cell.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-6R antibody [RM2071] (ab322899)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) cells labelling IL-6R with ab322899 at 1/50 (9.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic and membranous staining in RAW 264.7 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: WEHI-231 (PMID: 2112585)

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-6R antibody [RM2071] (ab322899), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-6R antibody [RM2071] (ab322899)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized U-937 (human histiocytic lymphoma monocyte) cells labelling IL-6R with ab322899 at 1/50 (9.5 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

    Confocal image showing cytoplasmic and membranous staining in U-937 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

    Negative control: K-562 (PMID: 8300137)

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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