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AB246805

Anti-IL-7 antibody [EPR6265] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL-7 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

Interleukin-7, IL-7, IL7

1 Images
Western blot - Anti-IL-7 antibody [EPR6265] - BSA and Azide free (AB246805)
  • WB

Supplier Data

Western blot - Anti-IL-7 antibody [EPR6265] - BSA and Azide free (AB246805)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab193358).

All lanes:

Western blot - Anti-IL-7 antibody [EPR6265] (<a href='/en-us/products/primary-antibodies/il-7-antibody-epr6265-ab193358'>ab193358</a>) at 1/20000 dilution

All lanes:

Human IL7 recombinant protein (153aa, 17.4kDa) at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 20 kDa

Observed band size: 17 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6265

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab246805 is the carrier-free version of ab193358.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-7 (IL-7) also known as IL-7 protein or IL-7 is a cytokine with a critical role in immune system development and function. IL-7 has an approximate molecular mass of 25 kDa and it exhibits expression in several tissues including stromal cells in the bone marrow and thymus. It interacts with its specific receptor IL-7R which consists of the IL-7Rα chain and the common gamma chain (γc) shared with other cytokine receptors. These interactions are important for signaling pathways that support lymphoid tissue homeostasis.
Biological function summary

IL-7 significantly influences lymphocyte development and survival. It is essential for the proliferation and maintenance of T cells particularly thymocytes during T cell maturation and peripheral T cells after they exit the thymus. IL-7 does not form a complex itself but works as a critical single molecule in these processes. IL-7's role is especially noted in the maintenance of naïve and memory T cells important for adaptive immunity.

Pathways

IL-7 activity impacts important immune signaling routes. The cytokine is an important player in the JAK/STAT signaling pathway which gets activated upon IL-7R engagement. This pathway is fundamental in controlling T cell development proliferation and survival. IL-7's interaction with other proteins such as the IL-7R complex and their downstream signaling partners integrates it into networks critical for strengthening the immune response.

IL-7 is significantly linked to immunodeficiencies and cancers affecting immune cells. For instance IL-7's altered signaling is associated with severe combined immunodeficiencies (SCID) where the lack of functional IL-7 or its receptor compromises T cell development. Additionally overexpression of IL-7 has connections to certain leukemias and lymphomas where it may contribute to inappropriate lymphocyte proliferation. In these contexts IL-7 often gets investigated alongside proteins such as the JAK and STAT family involved in tumorigenesis and immune dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Hematopoietic cytokine that plays an essential role in the development, expansion, and survival of naive and memory T-cells and B-cells thereby regulating the number of mature lymphocytes and maintaining lymphoid homeostasis (PubMed : 25870237, PubMed : 7527823). Mechanistically, exerts its biological effects through a receptor composed of IL7RA subunit and the cytokine receptor common subunit gamma/CSF2RG (PubMed : 8128231). Binding to the receptor leads to activation of various kinases including JAK1 or JAK3 depending on the cell type and subsequently propagation of signals through activation of several downstream signaling pathways including the PI3K/Akt/mTOR or the JAK-STAT5 (PubMed : 18523275, PubMed : 20974963).
See full target information IL7
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Product promise

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For full details, please see our Terms & Conditions

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