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AB18672

Anti-IL-8 antibody [807]

5

(9 Reviews)

|

(71 Publications)

Mouse Monoclonal IL-8 antibody. Suitable for WB and reacts with Human samples. Cited in 71 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

2 Images
Western blot - Anti-IL-8 antibody [807] (AB18672)
  • WB

Supplier Data

Western blot - Anti-IL-8 antibody [807] (AB18672)

This image was produced useing a version of the antibody producted in ascites.

All lanes:

Western blot - Anti-IL-8 antibody [807] (ab18672) at 0.1 µg/mL

All lanes:

recombinant human IL8, 100 ng

Predicted band size: 11 kDa

false

Western blot - Anti-IL-8 antibody [807] (AB18672)
  • WB

CiteAb

Western blot - Anti-IL-8 antibody [807] (AB18672)

IL-8 western blot using anti-IL-8 antibody [807] ab18672. Publication image and figure legend from Zhou, X., Lu, H., et al., 2020, Cell Mol Biol Lett, PubMed 32082390.

ab18672 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab18672 please see the product overview.

MiR-429 inhibited the NF-κB pathway. a, c and e – Relative mRNA expression of NF-κB-regulated genes in SH-SY5Y (a), SK-N-SH (c) and IMR-32 (e) cells. GAPDH was used as a loading control. b, d and f – Western blot showing NF-κB-regulated gene protein expression levels in SH-SY5Y (b), SK-N-SH (d) and IMR-32 (f) cells. g and h – Overexpression of IKKβ attenuated the anticancer effect of miR-429 in SH-SY5Y and SK-N-SH cells. Error bars represent the mean ± SEM of at least three independent experiments. N.S. : not significant; *p < 0.05 and **p < 0.01 vs. the control group

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

807

Isotype

IgG1

Light chain type

kappa

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCL8.

P10145

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.02-0.1 µg/mL", "WB-species-notes": "<p>Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8</p>" } } }

Product details

This product was changed from ascites to tissue culture supernatant on 12/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Purification notes
Purified from TCS.
Storage buffer
pH: 7.2 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8

Publications (71)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:2229 PubMed40044657

2025

p53 enhances DNA repair and suppresses cytoplasmic chromatin fragments and inflammation in senescent cells.

Applications

Unspecified application

Species

Unspecified reactive species

Karl N Miller,Brightany Li,Hannah R Pierce-Hoffman,Shreeya Patel,Xue Lei,Adarsh Rajesh,Marcos G Teneche,Aaron P Havas,Armin Gandhi,Carolina Cano Macip,Jun Lyu,Stella G Victorelli,Seung-Hwa Woo,Anthony B Lagnado,Michael A LaPorta,Tianhui Liu,Nirmalya Dasgupta,Sha Li,Andrew Davis,Anatoly Korotkov,Erik Hultenius,Zichen Gao,Yoav Altman,Rebecca A Porritt,Guillermina Garcia,Carolin Mogler,Andrei Seluanov,Vera Gorbunova,Susan M Kaech,Xiao Tian,Zhixun Dou,Chongyi Chen,João F Passos,Peter D Adams

Medicine 103:e39458 PubMed39465852

2024

Expression of interleukin-8 and integrin β3 predicts prognosis of patients with hepatocellular carcinoma after hepatectomy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiao Zhang,Yi Yin,Jiliang Tang,Mingze Ma,Huimin Shen,Yingrong Zhang,Fengkai Sun

Molecular cell 84:3271-3287.e8 PubMed39178863

2024

Histone chaperone HIRA, promyelocytic leukemia protein, and p62/SQSTM1 coordinate to regulate inflammation during cell senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Nirmalya Dasgupta,Xue Lei,Christina Huan Shi,Rouven Arnold,Marcos G Teneche,Karl N Miller,Adarsh Rajesh,Andrew Davis,Valesca Anschau,Alexandre R Campos,Rebecca Gilson,Aaron Havas,Shanshan Yin,Zong Ming Chua,Tianhui Liu,Jessica Proulx,Michael Alcaraz,Mohammed Iqbal Rather,Josue Baeza,David C Schultz,Kevin Y Yip,Shelley L Berger,Peter D Adams

Scientific reports 14:19078 PubMed39154102

2024

LncRNA GHET1 from bone mesenchymal stem cell-derived exosomes improves doxorubicin-induced pyroptosis of cardiomyocytes by mediating NLRP3.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoya Zhai,Jiedong Zhou,Xingxiao Huang,Jingfan Weng,Hui Lin,Shimin Sun,Jufang Chi,Liping Meng

British journal of cancer 131:271-282 PubMed38862740

2024

Tumour cell-derived serglycin promotes IL-8 secretion of CAFs in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Li,Guiping Xie,Jia Chen,Yaohui Wang,Jing Zhai,Lizong Shen

Cytotechnology 76:97-111 PubMed38304623

2024

Study on the mechanism of CXCL12/CXCR4-axis-mediated upregulation of IL-8 and IL-6 on the biological function of acute T lymphocyte leukaemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Zhou,Hui Zhao,Chao Zhang,Zhe Chen,Dong Li,Guanglei Qian

Biomedical optics express 15:185-198 PubMed38223184

2024

Functionalized optical fiber ball-shaped biosensor for label-free, low-limit detection of IL-8 protein.

Applications

Unspecified application

Species

Unspecified reactive species

Gyunel Rashidova,Meruyert Tilegen,Tri T Pham,Aliya Bekmurzayeva,Daniele Tosi

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2303489 PubMed37964763

2023

Branched-Chain Amino Acid Accumulation Fuels the Senescence-Associated Secretory Phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Yaosi Liang,Christopher Pan,Tao Yin,Lu Wang,Xia Gao,Ergang Wang,Holly Quang,De Huang,Lianmei Tan,Kun Xiang,Yu Wang,Peter B Alexander,Qi-Jing Li,Tso-Pang Yao,Zhao Zhang,Xiao-Fan Wang

Cell death & disease 14:725 PubMed37938546

2023

Crosstalk with lung fibroblasts shapes the growth and therapeutic response of mesothelioma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yakinthi Chrisochoidou,Rajat Roy,Pooyeh Farahmand,Guadalupe Gonzalez,Jennifer Doig,Lukas Krasny,Ella F Rimmer,Anne E Willis,Marion MacFarlane,Paul H Huang,Neil O Carragher,Alison F Munro,Daniel J Murphy,Kirill Veselkov,Michael J Seckl,Miriam F Moffatt,William O C Cookson,Olivier E Pardo

BMC immunology 24:4 PubMed36650424

2023

PU.1-CD23 signaling mediates pulmonary innate immunity against Aspergillus fumigatus infection by driving inflammatory response.

Applications

Unspecified application

Species

Unspecified reactive species

Min Wang,Ming Zhang,Jiayong Qiu,Chenyang Liu,Yao Lou,Tongsheng Wang,Yingmin Zhang,Yimin Mao
View all publications

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