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Mouse Monoclonal IL-8 antibody. Suitable for WB and reacts with Human samples. Cited in 59 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.


Images

Western blot - Anti-IL-8 antibody [807] (AB18672), expandable thumbnail
  • Western blot - Anti-IL-8 antibody [807] (AB18672), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Mouse
Storage buffer

pH: 7.2
Constituents: 0.0268% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Human CXCL8. Database link P10145

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WB
Human
Tested

Tested
Tested

Species
Human
Dilution info
0.02000-0.10000 µg/mL
Notes

Concentration of 0.02-0.1 ug/ml of ab18672 will allow visualization of 100 ng/lane of human IL-8

Associated Products

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Target data

Function

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).

Alternative names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

Recommended products

Mouse Monoclonal IL-8 antibody. Suitable for WB and reacts with Human samples. Cited in 59 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Full Length Protein corresponding to Human CXCL8. Database link P10145
Clone number
807
Purity
Tissue culture supernatant
Light chain type
kappa
Concentration
Loading...
Purification notes

Purified from TCS.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product was changed from ascites to tissue culture supernatant on 12/12/2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

Associated diseases and disorders

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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2 product images

  • Western blot - Anti-IL-8 antibody [807] (ab18672), expandable thumbnail

    Western blot - Anti-IL-8 antibody [807] (ab18672)

    This image was produced useing a version of the antibody producted in ascites.

    All lanes: Western blot - Anti-IL-8 antibody [807] (ab18672) at 0.1 µg/mL

    All lanes: recombinant human IL8, 100 ng

    Predicted band size: 11 kDa

  • Western blot - Anti-IL-8 antibody [807] (ab18672), expandable thumbnail

    Image collected and cropped by CiteAb under a CC-BY license from the publication

    Western blot - Anti-IL-8 antibody [807] (ab18672)

    IL-8 western blot using anti-IL-8 antibody [807] ab18672. Publication image and figure legend from Zhou, X., Lu, H., et al., 2020, Cell Mol Biol Lett, PubMed 32082390.


    ab18672 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab18672 please see the product overview.

    MiR-429 inhibited the NF-κB pathway. a, c and e – Relative mRNA expression of NF-κB-regulated genes in SH-SY5Y (a), SK-N-SH (c) and IMR-32 (e) cells. GAPDH was used as a loading control. b, d and f – Western blot showing NF-κB-regulated gene protein expression levels in SH-SY5Y (b), SK-N-SH (d) and IMR-32 (f) cells. g and h – Overexpression of IKKβ attenuated the anticancer effect of miR-429 in SH-SY5Y and SK-N-SH cells. Error bars represent the mean ± SEM of at least three independent experiments. N.S.: not significant; *p < 0.05 and **p < 0.01 vs. the control group

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