AB154390

Anti-IL-8 antibody

Name: Anti-IL-8 antibody (ab154390) | Abcam
Brand: Abcam Limited
SKU: AB154390
Price: 535 USD
Availability: InStock

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(13 Publications)

Rabbit Polyclonal IL-8 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 13 publications. Immunogen corresponding to Recombinant Fragment Protein within Human CXCL8 aa 1 to C-terminus.

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

4 Images

Supplier Data

Western blot - Anti-IL-8 antibody (AB154390)

Samples were separated by 15 % SDS.

All lanes:

Western blot - Anti-IL-8 antibody (ab154390) at 1/5000 dilution

Lane 1:

Non-transfected 293T whole cell extracts at 30 µg

Lane 2:

IL-8 transfected 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 11 kDa

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Supplier Data

Western blot - Anti-IL-8 antibody (AB154390)

Samples were separated by 15 % SDS.

All lanes:

Western blot - Anti-IL-8 antibody (ab154390) at 1/1000 dilution

Lane 1:

HepG2 whole cell extract at 30 µg

Lane 2:

HepG2 whole cell extract in conditioned medium at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 11 kDa

false

Supplier Data

Western blot - Anti-IL-8 antibody (AB154390)

Samples were separated by 15 % SDS.

All lanes:

Western blot - Anti-IL-8 antibody (ab154390) at 1/500 dilution

All lanes:

Human leukocyte) at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 11 kDa

false

CiteAb

Western blot - Anti-IL-8 antibody (AB154390)

IL-8 western blot using anti-IL-8 antibody ab154390. Publication image and figure legend from Lewinska, A., Adamczyk-Grochala, J., et al., 2020, Aging (Albany NY), PubMed 32584786.

ab154390 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab154390 please see the product overview.

Remifentanil preconditioning protects against hypoxia-induced senescence in HCM cells. (A) Senescence-associated β-galactosidase (SA-β-gal) activity. Representative microphotographs are shown. Scale bars 50 μm, objective 20×. The levels of SA-β-gal positive (blue) and negative (no blue) cells were calculated [%]. (B) Western blot analysis of the levels of cell cycle inhibitor p21. Data were normalized to β-actin. (C) Western blot analysis of the levels of pro-inflammatory cytokine IL-8. IL-8 levels in supernatants (Myocyte Growth Medium, MGM) were calculated per 10000 cells. Bars indicate SD, n = 3, ***p < 0.001 compared to normoxic control (CTR), ###p < 0.001, ##p < 0.01 compared to hypoxic control (CTR) (ANOVA and Dunnett's a posteriori test). CTR, control; R, remifentanil preconditioning.

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CXCL8 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P10145

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.

Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).

See full target information CXCL8

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Publications (13)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 25: PubMed38338930

2024

CXCL14 as a Key Regulator of Neuronal Development: Insights from Its Receptor and Multi-Omics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yinjie Zhang,Yue Jin,Jingjing Li,Yan Yan,Ting Wang,Xuanlin Wang,Zhenyu Li,Xuemei Qin

BMC pregnancy and childbirth 22:470 PubMed35668364

2022

Triclosan activates c-Jun/miR-218-1-3p/SLC35C1 signaling to regulate cell viability, migration, invasion and inflammatory response of trophoblast cells in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Huo,Ying Wang,Ting Chen,Tianyue Cao,Yue Zhang,Zhouhong Shi,Shunyu Hou

Cartilage 13:19476035221074024 PubMed35255737

2022

MiR-379-5p Promotes Chondrocyte Proliferation via Inhibition of PI3K/Akt Pathway by Targeting YBX1 in Osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Zhang,Wendi Zheng,Du Li,Jia Zheng

Cancers 14: PubMed35158873

2022

Senolysis-Based Elimination of Chemotherapy-Induced Senescent Breast Cancer Cells by Quercetin Derivative with Blocked Hydroxy Groups.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Lewińska,Paweł Przybylski,Jagoda Adamczyk-Grochala,Dominika Błoniarz,Grzegorz Litwinienko,Maciej Wnuk

Aging 13:15833-15874 PubMed34139673

2021

The lack of functional gene modulates cancer cell responses during drug-induced senescence.

Applications

Unspecified application

Species

Unspecified reactive species

Dominika Bloniarz,Jagoda Adamczyk-Grochala,Anna Lewinska,Maciej Wnuk

Experimental and therapeutic medicine 21:629 PubMed33936285

2021

Thioredoxin relieves lipopolysaccharide-induced acute kidney injury in mice by reducing inflammation, oxidative stress and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Jingjing Wang,Wenjuan Zhang,Guoyuan Lu

Molecular medicine reports 23: PubMed33760117

2021

miR‑302c‑3p and miR‑520a‑3p suppress the proliferation of cervical carcinoma cells by targeting CXCL8.

Applications

Unspecified application

Species

Unspecified reactive species

Hong-Mei Ding,Hong Zhang,Juan Wang,Jin-Hua Zhou,Fang-Rong Shen,Ru-Ning Ji,Jia-Yin Shi,You-Guo Chen

Aging 12:13924-13938 PubMed32584786

2020

Remifentanil preconditioning protects against hypoxia-induced senescence and necroptosis in human cardiac myocytes .

Applications

Unspecified application

Species

Unspecified reactive species

Anna Lewinska,Jagoda Adamczyk-Grochala,Dominika Bloniarz,Beata Horeczy,Slawomir Zurek,Arkadiusz Kurowicki,Bogumila Woloszczuk-Gebicka,Kazimierz Widenka,Maciej Wnuk

Redox biology 28:101337 PubMed31622846

2019

AMPK-mediated senolytic and senostatic activity of quercetin surface functionalized FeO nanoparticles during oxidant-induced senescence in human fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Lewinska,Jagoda Adamczyk-Grochala,Dominika Bloniarz,Jakub Olszowka,Magdalena Kulpa-Greszta,Grzegorz Litwinienko,Anna Tomaszewska,Maciej Wnuk,Robert Pazik

Cellular physiology and biochemistry : internation 52:94-108 PubMed30790507

2019

Linoleic Acid Increases Prostaglandin E2 Release and Reduces Mitochondrial Respiration and Cell Viability in Human Trophoblast-Like Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Nirajan Shrestha,James S M Cuffe,Olivia J Holland,Anthony V Perkins,Andrew J McAinch,Deanne H Hryciw

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IL-8 antibody

Western blot - Anti-IL-8 antibody (AB154390)

Western blot - Anti-IL-8 antibody (AB154390)

Western blot - Anti-IL-8 antibody (AB154390)

Western blot - Anti-IL-8 antibody (AB154390)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (13)

Product promise