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AB7747

Anti-IL-8 antibody

4

(3 Reviews)

|

(77 Publications)

Rabbit Polyclonal IL-8 antibody. Suitable for ELISA and reacts with Recombinant full length protein - Human samples. Cited in 77 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CXCL8.

View Alternative Names

IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1

1 Images
ELISA - Anti-IL-8 antibody (AB7747)
  • ELISA

Supplier Data

ELISA - Anti-IL-8 antibody (AB7747)

Specificity test of ab7747.

ab7747 was biotinylated and used as a detection antibody in different concentrations.

Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1 : 50.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CXCL8.

P10145

Specificity

Please be aware that this product has low homology with the mouse and rat sequence of IL8 (Rat, 46%; Mouse 45%, UniProt blast) and we therefore cannot guarantee reactivity in these species.

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Reactivity data

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
Biological function summary

IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.

Pathways

IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.

IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed : 18692776, PubMed : 7636208). Also plays an important role in neutrophil activation (PubMed : 2145175, PubMed : 9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed : 1840701, PubMed : 1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed : 11971003, PubMed : 8662698).
See full target information CXCL8
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Publications (77)

Recent publications for all applications. Explore the full list and refine your search

Anatolian journal of cardiology : PubMed39568422

2024

LncRNA TUG1 Knockdown Reduces Cardiomyocyte Damage in Viral Myocarditis by Targeting the miR-140-3p/CXCL8 Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Ji Shi,Qiyin Sun

Heliyon 9:e17366 PubMed37426804

2023

circSnd1 promotes atherosclerosis progression through the miR-485-3p/Olr1 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Yang,Yuhao Lin,Chao Wang,Pengcheng Fan

Journal of extracellular vesicles 12:e12342 PubMed37387557

2023

Stromal cell-derived small extracellular vesicles enhance radioresistance of prostate cancer cells via interleukin-8-induced autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Xiumei Wang,Fan Xu,Hengyuan Kou,Yawen Zheng,Jing Yang,Zhi Xu,Yao Fang,Wenbo Sun,Shuyi Zhu,Qin Jiang,Xiaowei Wei,Yong Xu

Cancer cell international 22:362 PubMed36403057

2022

Targeted blocking of CCR2 and CXCR2 improves the efficacy of transarterial chemoembolization of hepatocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiqiang Tian,Xiaojuan Hou,Wenting Liu,Changchun Shao,Lu Gao,Jinghua Jiang,Li Zhang,Zhipeng Han,Lixin Wei

Frontiers in cellular and infection microbiology 12:798978 PubMed35463647

2022

Junin Virus Activates p38 MAPK and HSP27 Upon Entry.

Applications

Unspecified application

Species

Unspecified reactive species

Collin J Fitzpatrick,Rajini R Mudhasani,Louis A Altamura,Catherine E Campbell,Julie P Tran,Brett F Beitzel,Aarthi Narayanan,Cynthia L de la Fuente,Kylene Kehn-Hall,Jeffrey M Smith,Connie S Schmaljohn,Aura R Garrison

Frontiers in oral health 2:649406 PubMed35048001

2021

Expression of Interleukin-1ß and Interleukin-8 in Oral Potentially Malignant Disorders and Carcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

Jeaneth Lopez-Labady,Ronell Bologna-Molina,Mariana Villarroel-Dorrego

Journal of molecular histology 52:671-680 PubMed33905072

2021

Nell-1 attenuates lipopolysaccharide-induced inflammation in human dental pulp cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rongyu Cao,Qiang Wang,Jiameng Wu,Mengmeng Liu,Qi Han,Xiaoying Wang

Frontiers in immunology 12:656350 PubMed33868301

2021

Lung Neutrophilic Recruitment and IL-8/IL-17A Tissue Expression in COVID-19.

Applications

Unspecified application

Species

Unspecified reactive species

Marina Luise Viola Azevedo,Aline Cristina Zanchettin,Caroline Busatta Vaz de Paula,Jarbas da Silva Motta Júnior,Mineia Alessandra Scaranello Malaquias,Sonia Mara Raboni,Plínio Cezar Neto,Rafaela Chiuco Zeni,Amanda Prokopenko,Nícolas Henrique Borges,Thiago Mateus Godoy,Ana Paula Kubaski Benevides,Daiane Gavlik de Souza,Cristina Pellegrino Baena,Cleber Machado-Souza,Lucia de Noronha

Cureus 13:e13499 PubMed33777584

2021

Proteomic Analysis of Cardioembolic and Large Artery Atherosclerotic Clots Using Reverse Phase Protein Array Technology Reveals Key Cellular Interactions Within Clot Microenvironments.

Applications

Unspecified application

Species

Unspecified reactive species

Mehdi Abbasi,Sean Fitzgerald,Jennifer Ayers-Ringler,Virginia Espina,Claudius Mueller,Sally Rucker,Ramanathan Kadirvel,David Kallmes,Waleed Brinjikji

Bosnian journal of basic medical sciences 21:81-92 PubMed32343942

2021

Association of miRNA-145 with the occurrence and prognosis of hydrosalpinx-induced defective endometrial receptivity.

Applications

Unspecified application

Species

Unspecified reactive species

Qingli Wang,Haiquan Ai,Xia Li,Haiqing Tian,Bingxue Ning,Meng Zhang,Xiaolin La
View all publications
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Product promise

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