Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture)

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling IL-8 with ab242515 at 1/2000 (0.501 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human cervical carcinoma. The section was incubated with ab242515 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IL8 KO PC-3 (IL-8 knock out human prostate adenocarcinoma epithelial cell) treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours (Megenta) or untreated with IL8 KO PC-3(Green)(Right) / PC-3 treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours(Megenta) or untreated with PC-3(Green)(Left) cells labelling IL-8 with ab242515 at 1/500 dilution (0.1µg) followed by a Goat Anti-Rabbit IgG (Alexa Fluor^® 488, ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized wildtype PC-3 and CXCL8 KO PC-3 (CXCL8 knockout human prostate adenocarcinoma epithelial cell), ab273743 cells labelling IL-8 with ab242515 at 1/500 (2.082 µg/ml) dilution followed by a ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg) dilution (Green).

Confocal image showing no staining in CXCL8 knockout PC-3 cells, showing weak cytoplasmic staining in wildype PC-3 cells (shown in green), and the wildtype PC-3 staining is increased after treated with Lipopolysaccharide (2 ug/ml) and Brefeldin A (5 µg/ml) for 5 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor^® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at a 1/1000 (2 µg) dilution.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

Immunohistochemical analysis of paraffin-embedded (A) : Untreated Wild-type PC-3 (human prostate adenocarcinoma epithelial cell) cell pellet; (B) : Wild-type PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet; (C) : Untreated CXCL8 knockout PC-3 cell pellet and (D) : CXCL8 knockout PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet labeling IL-8 with ab242515 at 1/2000 (0.501 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Weak staining on (A) untreated Wild-type PC-3 cell pellet, positive staining on (B) wild-type PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet, no staining on (C) untreated CXCL8 knockout PC-3 cell pellet and (D) CXCL8 knockout PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet.
The section was incubated with ab242515 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND^® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• sELISA

Supplier Data

Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab214030).