Rabbit Recombinant Monoclonal IL-8 antibody. Carrier free. Suitable for sELISA, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Monkey, Human samples.
Images
![Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--sandwich-elisa-img48085.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441719.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441720.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)")
![Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunocytochemistry-immunofluorescence-img441721.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)")
![Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--flow-cytometry-intracellular-img441722.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (AB242515)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | IHC-P | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|---|
| Human | Expected | Tested | Tested | Tested |
| Monkey | Expected | Predicted | Predicted | Predicted |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey, Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Monkey | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection (PubMed:18692776, PubMed:7636208). Also plays an important role in neutrophil activation (PubMed:2145175, PubMed:9623510). Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells (PubMed:1840701, PubMed:1891716). G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways (PubMed:11971003, PubMed:8662698).
Alternative names
IL8, CXCL8, Interleukin-8, IL-8, C-X-C motif chemokine 8, Chemokine (C-X-C motif) ligand 8, Emoctakin, Granulocyte chemotactic protein 1, Monocyte-derived neutrophil chemotactic factor, Monocyte-derived neutrophil-activating peptide, Neutrophil-activating protein 1, Protein 3-10C, T-cell chemotactic factor, GCP-1, MDNCF, MONAP, NAP-1
Recommended products
Rabbit Recombinant Monoclonal IL-8 antibody. Carrier free. Suitable for sELISA, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Monkey, Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR19358-37
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242515 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey IL-8. The recommended pair for sandwich ELISA is:
Capture: ab242515, Human/Monkey IL-8 Capture Antibody (unconjugated)
Detector: Anti-IL-8 antibody [EPR19358-108] - BSA and Azide free (Detector) ab242983, Human/Monkey IL-8 Detector Antibody (unconjugated)
The reference range value is 1.9 - 125 pg/ml for human and 4.7 - 300 pg/ml for monkey.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-8 also known as CXCL8 is a cytokine with a molecular weight of approximately 8 kDa. It belongs to the CXC chemokine family and plays a role in the recruitment of neutrophils to sites of inflammation. Cells like monocytes macrophages and endothelial cells express IL-8 after activation by pro-inflammatory signals. The protein exhibits high expression in cells associated with the immune response and inflammation serving as a signaling molecule between these cell types.
- Biological function summary
IL-8 functions as a chemoattractant for neutrophils and lymphocytes facilitating their movement towards the site of infection or injury. It does not form part of a larger protein complex but operates individually to enhance immune cell migration. IL-8 possesses unique binding motifs allowing it to interact with specific receptors namely CXCR1 and CXCR2 on target cells. This binding triggers cellular responses leading to effective immune surveillance and response to inflammatory stimuli.
- Pathways
IL-8 operates within important inflammatory and immune response pathways. It forms a part of the NF-κB signaling cascade which activates in response to stress signals promoting the expression of other inflammatory mediators. Additionally it engages in the mitogen-activated protein kinase (MAPK) pathway influencing cellular responses such as proliferation and differentiation. The interaction of IL-8 with these pathways highlights its role in modulating immune responses and highlights its interaction with other proteins such as TNF-α and IL-1β.
- Associated diseases and disorders
IL-8 shows a strong association with conditions like rheumatoid arthritis and chronic obstructive pulmonary disease (COPD). It acts as a biomarker for these diseases often correlating with disease severity and inflammation levels. In rheumatoid arthritis IL-8 contributes to joint inflammation and degradation while in COPD it enhances airway inflammation and tissue damage. The cytokine's involvement in these diseases connects it with other inflammatory mediators such as MMPs and leukotrienes which collectively exacerbate disease pathology.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--sandwich-elisa-img48085.jpg "Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)")
Sandwich ELISA - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Human IL-8 ELISA Kit ab214030).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441719.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)
Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling IL-8 with ab242515 at 1/2000 (0.501 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Positive staining on human cervical carcinoma. The section was incubated with ab242515 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img441720.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)
Immunohistochemical analysis of paraffin-embedded (A): Untreated Wild-type PC-3 (human prostate adenocarcinoma epithelial cell) cell pellet; (B): Wild-type PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet; (C): Untreated CXCL8 knockout PC-3 cell pellet and (D): CXCL8 knockout PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet labeling IL-8 with ab242515 at 1/2000 (0.501 µg/ml) dilution, followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Weak staining on (A) untreated Wild-type PC-3 cell pellet, positive staining on (B) wild-type PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet, no staining on (C) untreated CXCL8 knockout PC-3 cell pellet and (D) CXCL8 knockout PC-3 treated with 2µg/ml LPS for 1 hour, 5µg/ml Brefeldin A was then added for additional 5 hours, cell pellet.
The section was incubated with ab242515 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.
![Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--immunocytochemistry-immunofluorescence-img441721.jpg "Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)")
Immunocytochemistry/ Immunofluorescence - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized wildtype PC-3 and CXCL8 KO PC-3 (CXCL8 knockout human prostate adenocarcinoma epithelial cell), Human CXCL8 knockout PC-3 cell line ab273743 cells labelling IL-8 with ab242515 at 1/500 (2.082 µg/ml) dilution followed by a Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution (Green).
Confocal image showing no staining in CXCL8 knockout PC-3 cells, showing weak cytoplasmic staining in wildype PC-3 cells (shown in green), and the wildtype PC-3 staining is increased after treated with Lipopolysaccharide (2 ug/ml) and Brefeldin A (5 µg/ml) for 5 hours. The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (2.5µg/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at a 1/1000 (2 µg) dilution.
![Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515), expandable thumbnail](https://content.abcam.com/products/images/il-8-antibody-epr19358-37-bsa-and-azide-free-capture-ab242515--flow-cytometry-intracellular-img441722.jpg "Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)")
Flow Cytometry (Intracellular) - Anti-IL-8 antibody [EPR19358-37] - BSA and Azide free (Capture) (ab242515)
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized IL8 KO PC-3 (IL-8 knock out human prostate adenocarcinoma epithelial cell) treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours (Megenta) or untreated with IL8 KO PC-3(Green)(Right) / PC-3 treated with 2µg/ml LPS for 5hours and 5µg/ml Brefeldin A for 5 hours(Megenta) or untreated with PC-3(Green)(Left) cells labelling IL-8 with ab242515 at 1/500 dilution (0.1µg) followed by a Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at a 1/5000 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
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