AB203386

Anti-IL-9 antibody

Name: Anti-IL-9 antibody (ab203386) | Abcam
Brand: Abcam Limited
SKU: AB203386
Rating: 5 (1 reviews)

(1 Review)

(6 Publications)

Rabbit Polyclonal IL-9 antibody. Suitable for WB, IHC-P and reacts with Mouse samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Mouse Il9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, Il9

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody (AB203386)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody (AB203386)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse lung tissue labeling IL9 with ab203386 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-IL-9 antibody (AB203386)

All lanes:

Western blot - Anti-IL-9 antibody (ab203386) at 1/300 dilution

All lanes:

Mouse lung tissue lysate

Predicted band size: 15 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Mouse Il9 aa 1-100 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15247

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>(or 1/50 - 1/200 using a fluorescent secondary antibody).</p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.

Biological function summary

IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.

Pathways

Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.

IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed : 11070175, PubMed : 19433802). Affects intestinal epithelial permeability and adaptive immunity (PubMed : 12704113). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells (PubMed : 11070175, PubMed : 19433802). Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG (PubMed : 2145361, PubMed : 7718508). Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs (PubMed : 10464327). Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5 (PubMed : 10464327).

See full target information Il9

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of cardiovascular pharmacology 79:791-798 PubMed35666994

2022

Role of Bone Morphogenetic Protein 4 in the Inflammation of the Myocardium and Vascular Tissue of Obese Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wu,Liang Chen,Qin Shen,Li Wang,Gang-Jun Zong

Journal of neuroinflammation 18:108 PubMed33971906

2021

IL-9-triggered lncRNA Gm13568 regulates Notch1 in astrocytes through interaction with CBP/P300: contribute to the pathogenesis of experimental autoimmune encephalomyelitis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaomei Liu,Feng Zhou,Weixiao Wang,Guofang Chen,Qingxiu Zhang,Ruixue Lv,Zijun Zhao,Xiangyang Li,Qian Yu,Jessica M Meves,Hui Hua,Xiaocui Li,Xiaotian Wang,Hong Sun,Dianshuai Gao

Hypertension in pregnancy 39:228-235 PubMed32329646

2020

Decreased placental IL9 and IL9R in preeclampsia impair trophoblast cell proliferation, invasion, and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Yi Sun,Siyu Liu,Rong Hu,Qiongjie Zhou,Xiaotian Li

Oncology letters 20:299-307 PubMed32565956

2020

miR-208b-5p inhibits invasion of non-small cell lung cancer through the STAT3 pathway by targeting interleukin-9.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Ma,Hong-Feng Tong,Jie-Huan Lin,Fu-Nan Chen,Can-Xing Wu,Cheng-Zhang Cao,Jian Wu,Shu-Qiao Hu

Immunology 158:296-303 PubMed31436861

2019

Interleukin-9 blockage reduces early hepatic granuloma formation and fibrosis during Schistosoma japonicum infection in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Tingzheng Zhan,Huihui Ma,Suqin Jiang,Zirong Zhong,Xiaoli Wang,Chunxiang Li,Dan Yu,Lei Liu,Jing Xu,Chaoming Xia

OncoTargets and therapy 12:2225-2234 PubMed30988627

2019

Effect of Th9/IL-9 on the growth of gastric cancer in nude mice.

Applications

Unspecified application

Species

Unspecified reactive species

Li Cai,Yue Zhang,Yifei Zhang,Hongbing Chen,Jinchen Hu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL-9 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL-9 antibody (AB203386)

Western blot - Anti-IL-9 antibody (AB203386)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

Product promise