JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB227037

Anti-IL-9 antibody [EPR23484-151]

Be the first to review this product! Submit a review

|

(3 Publications)

Rabbit Recombinant Monoclonal IL-9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

View Alternative Names

Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, IL9

6 Images
Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (AB227037)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (AB227037)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.

Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • IP

Unknown

Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (AB227037)

IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10μg with ab227037 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : Mouse spleen tissue lysate 10μg

Lane 2 : ab227037 IP in mouse spleen tissue lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab227037 in mouse spleen tissue lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This blot was developed using a higher sensitivity ECL substrate.

All lanes:

Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037)

Predicted band size: 15 kDa

false

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • WB

Lab

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9806735).

Exposure time : 3 minutes.

All lanes:

Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

Lane 1:

Mouse spleen tissue lysate at 60 µg

Lane 2:

Mouse thymus tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 30 kDa

false

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • WB

Lab

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9806735).

Exposure time : 104 seconds.

All lanes:

Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

Lane 1:

Rat spleen tissue lysate at 60 µg

Lane 2:

Rat thymus tissue lysate at 60 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 15 kDa

Observed band size: 30 kDa

false

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)
  • WB

Lab

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 9806735).

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

All lanes:

His-tagged mouse IL9 recombinant protein, 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 15 kDa

Observed band size: 30 kDa

false

  • Carrier free

    Anti-IL-9 antibody [EPR23484-151] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23484-151

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
style
left-column

Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.
Biological function summary

IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.

Pathways

Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.

IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed : 29742432). Affects intestinal epithelial permeability and adaptive immunity (PubMed : 29742432). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs. Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5.
See full target information IL9
style
left-column

Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 14: PubMed39199394

2024

Association of IL-9 Cytokines with Hepatic Injury in Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Tanfang Zhou,Xinlu Xu,Jiang Zhu,Mayire Aizezi,Aili Aierken,Menggen Meng,Rongdong He,Kalibixiati Aimulajiang,Hao Wen

Acta pharmacologica Sinica 45:1951-1963 PubMed38760543

2024

Bevacizumab induces ferroptosis and enhances CD8 T cell immune activity in liver cancer via modulating HAT1 and increasing IL-9.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Yu Hou,Pan Lv,Hong-Feng Yuan,Li-Na Zhao,Yu-Fei Wang,Hui-Hui Zhang,Guang Yang,Xiao-Dong Zhang

Circulation research 130:1662-1681 PubMed35440172

2022

Perivascular Fibrosis Is Mediated by a KLF10-IL-9 Signaling Axis in CD4+ T Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rulin Zhuang,Jingshu Chen,Henry S Cheng,Carmel Assa,Anurag Jamaiyar,Arvind K Pandey,Daniel Pérez-Cremades,Bofang Zhang,Aspasia Tzani,Akm Khyrul Wara,Jorge Plutzky,Victor Barrera,Preetida Bhetariya,Richard N Mitchell,Zhongmin Liu,Mark W Feinberg
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Assay Kits

AB242237

Human IL-9 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human IL-9 ELISA Kit (AB242237)

  • 0
  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB15580

Anti-Ki67 antibody

BOND RX™ Validated|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody (AB15580)

  • 5
  • 230
Primary Antibodies

AB64693

Anti-Mannose Receptor antibody

BOND RX™ Validated

Western blot - Anti-Mannose Receptor antibody (AB64693)

  • 5
  • 42
Primary Antibodies

AB68153

Anti-STAT3 antibody [EPR787Y]

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-STAT3 antibody [EPR787Y] (AB68153)

  • 4
  • 10

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com