Skip to main content

Rabbit Recombinant Monoclonal IL-9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Be the first to review this product! Submit a review

Images

Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (AB227037), expandable thumbnail
  • Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (AB227037), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (AB227037), expandable thumbnail
  • Western blot - Anti-IL-9 antibody [EPR23484-151] (AB227037), expandable thumbnail

Publications

Key facts

Isotype
IgG1
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPWBIHC-PICC/IFFlow Cyt (Intra)
Human
Expected
Not recommended
Not recommended
Tested
Tested
Mouse
Tested
Tested
Not recommended
Expected
Expected
Rat
Expected
Tested
Not recommended
Expected
Expected

Tested
Tested

Species
Mouse
Dilution info
1/30
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000
Notes

-

Species
Rat
Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500
Notes

-

Expected
Expected

Species
Rat
Dilution info
-
Notes

-

Species
Mouse
Dilution info
1/500
Notes

-

Target data

Function

Multifunctional cytokine secreted mainly by T-helper 2 lymphocytes and also mast cells or NKT cells that plays important roles in the immune response against parasites (PubMed:29742432). Affects intestinal epithelial permeability and adaptive immunity (PubMed:29742432). In addition, induces the differentiation of specific T-cell subsets such as IL-17 producing helper T-cells (TH17) and also proliferation and differentiation of mast cells. Mechanistically, exerts its biological effects through a receptor composed of IL9R subunit and a signal transducing subunit IL2RG. Receptor stimulation results in the rapid activation of JAK1 and JAK3 kinase activities leading to STAT1, STAT3 and STAT5-mediated transcriptional programs. Induction of differentiation genes seems to be mediated by STAT1 alone, while protection of cells from apoptosis depends on STAT3 and STAT5.

Alternative names

Interleukin-9, IL-9, Cytokine P40, T-cell growth factor P40, IL9

Recommended products

Rabbit Recombinant Monoclonal IL-9 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

Key facts

Isotype
IgG1
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR23484-151
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Interleukin-9 (IL-9) sometimes known as a T-cell growth factor is a cytokine with a molecular mass of approximately 14-19 kDa. It is primarily expressed by T helper cells particularly Th2 and Th9 cells. IL-9 comes from the IL-9 gene and plays a significant role in the immune system by affecting various cell types such as mast cells B cells and epithelial cells. Researchers first identified it in the late 1980s and it has continued to be an area of interest due to its diverse functions.

Biological function summary

IL-9 influences immune responses and is important for various processes including cell proliferation and survival. IL-9 activates mast cells which play role in allergy response. It also affects the growth and differentiation of T cells and enhances the function of dendritic cells. IL-9 operates individually and not as part of a larger complex but works closely with its receptor IL-9R to initiate downstream signaling events.

Pathways

Cytokine signaling pathways and JAK-STAT signaling pathways integrate IL-9's function in the immune system. IL-9 activates the JAK-STAT pathway particularly involving proteins like JAK1 and STATs to relay signals that promote immune cell growth and survival. This cytokine also interlinks with other cytokines such as IL-4 and IL-13 playing role in immune regulation and inflammatory responses.

Associated diseases and disorders

IL-9 shows strong correlation with asthma and certain types of cancers particularly Hodgkin's lymphoma. In asthma overproduction of IL-9 can lead to increased mucus production and airway hyperresponsiveness. This relationship makes it a potential target for blocking therapies in asthma treatment. Higher levels of IL-9 correlate with poorer prognosis in some cancers indicating its potential role in tumor growth and immune evasion possibly interacting with IL-6 in such conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 104 seconds.

    All lanes: Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

    Lane 1: Rat spleen tissue lysate at 60 µg

    Lane 2: Rat thymus tissue lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 15 kDa

    Observed band size: 30 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2 μg/ml dilution (Green). Confocal image showing strong membranous and cytoplasmic staining in 293T cells transfected with HA tagged mouse IL9 construct. anti-HA.11 Epitope Tag mouse monoclonal antibody (Alexa Fluor® 647) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 2 μg/ml dilution.

  • Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    IL-9 was immunoprecipitated from 0.35 mg mouse spleen tissue lysate 10μg with ab227037 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab227037 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: Mouse spleen tissue lysate 10μg

    Lane 2: ab227037 IP in mouse spleen tissue lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227037 in mouse spleen tissue lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This blot was developed using a higher sensitivity ECL substrate.

    All lanes: Immunoprecipitation - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Predicted band size: 15 kDa

  • Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Intracellular flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized 293T (Human embryonic kidney epithelial cell) transfected with HA tagged mouse IL9 construct cells labelling IL-9 with ab227037 at 1/500 dilution (0.1μg) (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left). A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    This blot was developed using a higher sensitivity ECL substrate.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 3 minutes.

    All lanes: Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

    Lane 1: Mouse spleen tissue lysate at 60 µg

    Lane 2: Mouse thymus tissue lysate at 60 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 15 kDa

    Observed band size: 30 kDa

  • Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037), expandable thumbnail

    Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 9806735).

    Exposure time: 10 seconds.

    All lanes: Western blot - Anti-IL-9 antibody [EPR23484-151] (ab227037) at 1/1000 dilution

    All lanes: His-tagged mouse IL9 recombinant protein, 10 ng

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Predicted band size: 15 kDa

    Observed band size: 30 kDa

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com