AB238457

Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal IL1 Receptor I/IL-1R-1 antibody. Carrier free. Suitable for WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

CD121a, Il-1r1, Il1ra, Il1r1, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, p80, IL-1R-alpha

3 Images

Unknown

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

Blocking and dilution buffer : 5% NFDM/TBST .

Exposure times.

Lane 1 : 37 seconds, Lane 2 : 3 minutes.

The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID : 17324958, PMID : 28450382).

Negative control : B16-F10 (PMID : 29445180).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).

All lanes:

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (<a href='/en-us/products/primary-antibodies/il1-receptor-i-il-1r-1-antibody-epr22198-36-ab229051'>ab229051</a>) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 20 µg

Lane 2:

B16-F10 (mouse melanoma mixture of spindle-shaped and epithelial-like cells) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa,80 kDa

false

Unknown

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).

All lanes:

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (<a href='/en-us/products/primary-antibodies/il1-receptor-i-il-1r-1-antibody-epr22198-36-ab229051'>ab229051</a>) at 1/1000 dilution

Lane 1:

EL4.IL-2 (Mouse lymphoma T lymphocyte) cell lysate at 10 µg

Lane 2:

bEnd.3 (Mouse brain endothelioma ) cell lysate at 10 µg

Lane 3:

NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 65 kDa

Observed band size: 80 kDa

false

Unknown

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229051).

Blocking and dilution buffer : 5% NFDM/TBST.

Exposure time :

Lane 1 : 48 seconds

Lane 2 : 15 seconds

Lane 3 : 114 seconds.

The 80 kDa band represents the membrane form, while the 65 kDa band is the soluble form (PMID : 17324958, PMID : 28450382).

This blot was developed using a high sensitivity ECL substrate. The high-sensitivity ECL substrate used allows for the detection of proteins in the mid-femtogram range.

All lanes:

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] (<a href='/en-us/products/primary-antibodies/il1-receptor-i-il-1r-1-antibody-epr22198-36-ab229051'>ab229051</a>) at 1/1000 dilution

Lane 1:

Mouse lung lysate at 20 µg

Lane 2:

EL4.IL-2 (mouse lymphoma T lymphocyte cell line) whole cell lysate at 20 µg

Lane 3:

bEnd.3 (mouse brain endothelioma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa,80 kDa

true

Unconjugated

Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22198-36

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab238457 is the carrier-free version of ab229051.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL-1 Receptor I also known as IL-1R1 is a transmembrane protein with alternative names such as IL-1 receptor and IL-1 type I receptor. It acts as an important receptor for the pro-inflammatory cytokine Interleukin-1 (IL-1) family primarily IL-1α and IL-1β. IL-1R1 has a mass of approximately 80 kilodaltons and is expressed in a variety of cell types including endothelial cells epithelial cells and fibroblasts. It is also present on immune cells like monocytes and macrophages making it widespread in a vast range of tissues.

Biological function summary

IL-1R1 is a critical player in the initiation of immune responses serving as a component of the IL-1 signaling complex. When IL-1 binds to IL-1R1 it recruits the IL-1 receptor accessory protein (IL-1RAcP) to form a signaling complex that triggers downstream pathways. This interaction initiates a signaling cascade that activates nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs) promoting the expression of inflammatory genes and mediating immune and inflammatory responses.

Pathways

IL-1R1 is central to the inflammatory response and immune signaling pathways. The IL-1 receptor complex facilitates the activation of NF-κB which plays a role in the body's response to stress cytokines and pathogens. The receptor acts in close relation with other proteins like IL-1RAcP and IRAKs (Interleukin-1 receptor-associated kinases) in these pathways creating a robust immune signaling network that drives the inflammatory response.

IL-1R1 is closely associated with inflammatory conditions such as rheumatoid arthritis and inflammatory bowel disease. Increased IL-1R1 expression and signaling contribute to chronic inflammation in these diseases. The interaction of IL-1β with IL-1R1 and subsequently with proteins like NF-κB leads to the persistence of inflammatory signals. Moreover targeting IL-1R1 and related proteins offers potential therapeutic strategies in mitigating the symptoms and progression of inflammatory disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (By similarity).. Isoform 2. Unable to mediate canonical IL-1 signaling. Cooperates with IL1RAP isoform 3 to mediate IL1B-induced neuronal activity including IL1B-potentiated NMDA-induced calcium influx mediated by Akt kinase activation.

See full target information Il1r1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, and immunity 80:247-254 PubMed30885840

2019

Methamphetamine-associated cognitive decline is attenuated by neutralizing IL-1 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Arkadiusz Liśkiewicz,Marta Przybyła,Minseon Park,Daniela Liśkiewicz,Marta Nowacka-Chmielewska,Andrzej Małecki,Jarosław Barski,Joanna Lewin-Kowalik,Michal Toborek

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [EPR22198-36] - BSA and Azide free (AB238457)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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