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Rabbit Recombinant Multiclonal IL1 Receptor I/IL-1R-1 antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

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Images

Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (AB322149), expandable thumbnail
  • Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (AB322149), expandable thumbnail
  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (AB322149), expandable thumbnail
  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (AB322149), expandable thumbnail
  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (AB322149), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Multiclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIPICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info
1/1000
Notes

-

Species

Mouse

Dilution info
1/1000
Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info
1/30
Notes

-

Species

Mouse

Dilution info
1/30
Notes

-

Not recommended
Not recommended

Species

Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat

Dilution info

-

Notes

-

Target data

Function

Receptor for IL1A, IL1B and IL1RN (PubMed:2950091, PubMed:37315560). After binding to interleukin-1 associates with the coreceptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Involved in IL1B-mediated costimulation of IFNG production from T-helper 1 (Th1) cells (PubMed:10653850).

Alternative names

CD121a, IL1R, IL1RA, IL1RT1, IL1R1, Interleukin-1 receptor type 1, IL-1R-1, IL-1RT-1, IL-1RT1, CD121 antigen-like family member A, Interleukin-1 receptor alpha, Interleukin-1 receptor type I, p80, IL-1R-alpha

Rabbit Recombinant Multiclonal IL1 Receptor I/IL-1R-1 antibody. Suitable for WB, IP and reacts with Human, Mouse samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Multiclonal

Immunogens
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

RM1242

Purification technique

Affinity purification Protein A

Specificity

Not suitable for mouse IHC-P

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant multiclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149), expandable thumbnail

    Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149)

    IL1 Receptor I/IL-1R-1 was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast) whole cell lysate with ab322149 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322149 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 2: ab322149 IP in NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322149 in NIH/3T3 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149) at 1/30 dilution

    All lanes: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149), expandable thumbnail

    Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149)

    IL1 Receptor I/IL-1R-1 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate with ab322149 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab322149 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate

    Lane 2: ab322149 IP in HDLM-2 (human Hodgkin lymphoma) whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab322149 in HDLM-2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST

    All lanes: Immunoprecipitation - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149) at 1/30 dilution

    All lanes: HDLM-2 (human Hodgkin lymphoma) whole cell lysate

    Secondary

    All lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/5000 dilution

    Exposure time: 180s

  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149), expandable thumbnail

    Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: spleen, HL-1.

    Negative control: B16-F10 (PMID: 29445180).

    This blot of lane 5-6 was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    Exposure time: Lane 1-4: 180 seconds Lane 5-6: 114 seconds

    All lanes: Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149) at 1/1000 dilution

    Lane 1: EL4.IL-2 (mouse lymphoma T lymphocyte) whole cell lysate at 20 µg

    Lane 2: HL-1 (mouse atrial muscle cell) whole cell lysate at 20 µg

    Lane 3: B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

    Lane 4: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 5: Mouse spleen tissue lysate at 20 µg

    Lane 6: Mouse skeletal muscle tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 50-80 kDa, 36 kDa

  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149), expandable thumbnail

    Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Low expression: cerebellum and skeletal muscle.

    The identity of the higher MW band at approximately 80 kDa is membrane-bond form of IL1R1 and the lower MW band at approximately 50 kDa is soluble(s) form of IL1R1. The bands between the 50 to 80 kDa are expected to be degradation fragments (PMID: 17324958).

    This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149) at 1/1000 dilution

    Lane 1: Human cerebellum tissue lysate at 20 µg

    Lane 2: Human liver tissue lysate at 20 µg

    Lane 3: Human skeletal muscle tissue lysate at 20 µg

    Lane 4: Human spleen tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Observed band size: 50-80 kDa, 36 kDa

    Exposure time: 180s

  • Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149), expandable thumbnail

    Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149)

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Negative control: HT-1080, 293T (PMID: 18996842).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-IL1 Receptor I/IL-1R-1 antibody [RM1242] (ab322149) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg

    Lane 2: HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 3: HT-1080 (human fibrosarcoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 80 kDa, 36 kDa

    Exposure time: 180s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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