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AB314045

Anti-IL18R1 antibody [BLR172J]- BSA free

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Rabbit Recombinant Monoclonal IL-18R1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL18R1 aa 1-350.

View Alternative Names

CD218a, IL1RRP, IL18R1, Interleukin-18 receptor 1, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, Interleukin-18 receptor alpha, IL-1Rrp, IL1R-rp, IL-18R-alpha, IL-18Ralpha

2 Images
Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)

Detection of human IL18R1 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow Cytometry (Intracellular). Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells. Antibody : Standard version of ab314045 (shaded red) or isotype control (unshaded). Secondary : DyLight® 650-conjugated goat anti-rabbit IgG.

Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)
  • WB

Supplier Data

Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)

Samples : Whole cell lysate (25 µg) from NK-92, HEK293T, HDLM-2, NCI-H226, and NCI-H460 cells prepared using NETN lysis buffer. Antibody : Rabbit anti-IL18R1 recombinant monoclonal antibody [BLR172J] (A700-172 lot 1) used at 1 : 1000. Secondary : HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel : Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).

All lanes:

Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045) at 1/1000 dilution

Lane 1:

NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 25 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 25 µg

Lane 3:

HDLM-2 (Human Hodgkin's lymphoma cell line) whole cell lysate at 25 µg

Lane 4:

NCI-H266 (Human cell line) whole cell lysate at 25 µg

Lane 5:

NCI-H460 (Human cell line) whole cell lysate at 25 µg

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

BLR172J

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Fragment Protein within Human IL18R1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information.

Q13478

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 98% Borate buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
Up to 12 months
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B (PubMed : 14528293, PubMed : 25261253, PubMed : 25500532, PubMed : 37993714, PubMed : 8626725). Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (PubMed : 10653850). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).
See full target information IL18R1
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Product promise

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For full details, please see our Terms & Conditions

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