Rabbit Recombinant Monoclonal IL-18R1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL18R1 aa 1-350.
Images
![Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045), expandable thumbnail](https://content.abcam.com/products/images/il18r1-antibody-blr172j-bsa-free-ab314045--western-blot-img420367.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)")
![Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045), expandable thumbnail](https://content.abcam.com/products/images/il18r1-antibody-blr172j-bsa-free-ab314045--flow-cytometry-intracellular-img420368.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (AB314045)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human IL18R1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13478
Reactivity data
Select an application| Flow Cyt (Intra) | WB | |
|---|---|---|
| Human | Tested | Tested |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Within the IL18 receptor complex, responsible for the binding of the pro-inflammatory cytokine IL18, but not IL1A nor IL1B (PubMed:14528293, PubMed:25261253, PubMed:25500532, PubMed:37993714, PubMed:8626725). Involved in IL18-mediated IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). Contributes to IL18-induced cytokine production, either independently of SLC12A3, or as a complex with SLC12A3 (By similarity).
Alternative names
CD218a, IL1RRP, IL18R1, Interleukin-18 receptor 1, IL-18R-1, IL-18R1, CD218 antigen-like family member A, CDw218a, IL1 receptor-related protein, Interleukin-18 receptor alpha, IL-1Rrp, IL1R-rp, IL-18R-alpha, IL-18Ralpha
Recommended products
Rabbit Recombinant Monoclonal IL-18R1 antibody. Suitable for Flow Cyt (Intra), WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL18R1 aa 1-350.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.8 - 8.6
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human IL18R1 aa 1-350. The exact immunogen used to generate this antibody is proprietary information. Database link Q13478
- Clone number
- BLR172J
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- Up to 12 months
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045), expandable thumbnail](https://content.abcam.com/products/images/il18r1-antibody-blr172j-bsa-free-ab314045--western-blot-img420367.jpg "Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045)")
Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045)
Samples: Whole cell lysate (25 µg) from NK-92, HEK293T, HDLM-2, NCI-H226, and NCI-H460 cells prepared using NETN lysis buffer. Antibody:Rabbit anti-IL18R1 recombinant monoclonal antibody [BLR172J] (A700-172 lot 1) used at 1:1000. Secondary:HRP-conjugated goat anti-rabbit IgG (A120-101P). Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody [BLR057F] (A700-057).
All lanes: Western blot - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045) at 1/1000 dilution
Lane 1: NK-92 (Human peripheral blood malignant non-Hodgkin's lymphoma cell line) whole cell lysate at 25 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 25 µg
Lane 3: HDLM-2 (Human Hodgkin's lymphoma cell line) whole cell lysate at 25 µg
Lane 4: NCI-H266 (Human cell line) whole cell lysate at 25 µg
Lane 5: NCI-H460 (Human cell line) whole cell lysate at 25 µg
SecondaryAll lanes: HRP-conjugated goat anti-rabbit IgG
![Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045), expandable thumbnail](https://content.abcam.com/products/images/il18r1-antibody-blr172j-bsa-free-ab314045--flow-cytometry-intracellular-img420368.jpg "Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045)")
Flow Cytometry (Intracellular) - Anti-IL18R1 antibody [BLR172J]- BSA free (ab314045)
Detection of human IL18R1 in Jurkat (Human T cell leukemia cell line from peripheral blood) cells by Flow Cytometry (Intracellular).
Fixed in 4% formaldehyde and permeabilized with 90% methanol. 2 µl per 1 x 10^6 cells.
Antibody: Standard version of ab314045 (shaded red) or isotype control (unshaded).
Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Downloads
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