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AB264075

Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free

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Rabbit Recombinant Monoclonal IL2 Receptor beta/p75 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.

View Alternative Names

CD122, IL15RB, IL2RB, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75, p75

2 Images
Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (AB264075)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (AB264075)

Flow cytometric analysis of Human peripheral blood mononuclear cell (PBMC) cells labelling IL2 Receptor beta/p75 with ab263906 at 1/500 compared with a Rabbit monoclonal IgG (ab172730) isotype control (left) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Cells were stained with rabbit IgG (Left) or ab263906 (Right). Then stained with anti-CD56 conjugated to BV421. IL2 Receptor beta/CD122 is constitutively expressed primarily by NK cells (PMID : 24055329). Gated on viable cells.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263906).

Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (AB264075)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL2 Receptor beta/p75 antibody [EPR22892-2] - BSA and Azide free (AB264075)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized None cells labelling IL2 Receptor beta/p75 with ab263906 at 1/50 dilution, followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in a subset of Human PBMC cells. is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab263906).

  • Unconjugated

    Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]

  • 519 Alexa Fluor® 488

    Alexa Fluor® 488 Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]

  • 665 Alexa Fluor® 647

    Alexa Fluor® 647 Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]

  • 578 PE

    PE Anti-IL2 Receptor beta/p75 antibody [EPR22892-2]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22892-2

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Product details

ab264075 is the carrier-free version of ab263906.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL2 Receptor beta also known as IL-2R beta IL2R beta or CD122/IL2RB protein is a component of the interleukin-2 receptor complex. It is part of the receptor system for the cytokine IL-2. This receptor subunit has a molecular mass of approximately 75 kDa and gets alternatively referred to as IL2R beta or p75. It is expressed on the surface of activated T cells natural killer (NK) cells and in some cases myeloid lineage cells. This expression pattern correlates IL2R beta's role in essential immune functions and signaling processes.
Biological function summary

IL2R beta plays a critical role in cytokine binding and signal transduction for the interleukin-2 receptor which is a heterodimeric complex. This complex consists of the IL-2 receptor alpha (CD25) IL-2 receptor beta and a common gamma chain (γc CD132). The presence of IL-2R beta allows the receptor to mediate biological processes like cell proliferation differentiation and survival particularly within the immune system. The extent to how IL2R beta integrates into these processes makes it an important player in immune regulation and homeostasis.

Pathways

IL2R beta serves essential functions in the JAK-STAT and PI3K-AKT pathways. The binding of IL-2 to its receptor initiates a cascade of intracellular events which involve Janus kinases (JAKs) and signal transducer and activator of transcription (STATs) leading to transcriptional activation of various genes. Along with these pathways IL2R beta also interacts collaboratively with the gamma chain (γc) in promoting Downstream signaling events which enhance cell proliferation and survival.

IL2R beta's function has implications in autoimmune diseases and certain types of leukemia. Alterations or dysregulation in IL-2R signaling pathways often play a role in autoimmune conditions due to its importance in maintaining immune tolerance. Moreover given IL-2 receptor's role in T-cell and NK-cell activity aberrations may contribute to uncontrolled cellular proliferation seen in leukemia. Understanding its interplay with the common gamma chain (CD132) can provide insights into therapeutic interventions targeting these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed : 15123770, PubMed : 31040185).
See full target information IL2RB
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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