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Rabbit Recombinant Monoclonal IL2 Receptor beta/p75 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283), expandable thumbnail
  • Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283), expandable thumbnail
  • Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Not recommended
Tested
Tested
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Not recommended

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Mouse
Dilution info
-
Notes

-

Species
Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Target data

Function

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed:15123770, PubMed:31040185).

Alternative names

Recommended products

Rabbit Recombinant Monoclonal IL2 Receptor beta/p75 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR24336-29
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab282283 is the carrier-free version of Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The IL2 Receptor beta also known as IL-2R beta IL2R beta or CD122/IL2RB protein is a component of the interleukin-2 receptor complex. It is part of the receptor system for the cytokine IL-2. This receptor subunit has a molecular mass of approximately 75 kDa and gets alternatively referred to as IL2R beta or p75. It is expressed on the surface of activated T cells natural killer (NK) cells and in some cases myeloid lineage cells. This expression pattern correlates IL2R beta's role in essential immune functions and signaling processes.

Biological function summary

IL2R beta plays a critical role in cytokine binding and signal transduction for the interleukin-2 receptor which is a heterodimeric complex. This complex consists of the IL-2 receptor alpha (CD25) IL-2 receptor beta and a common gamma chain (γc CD132). The presence of IL-2R beta allows the receptor to mediate biological processes like cell proliferation differentiation and survival particularly within the immune system. The extent to how IL2R beta integrates into these processes makes it an important player in immune regulation and homeostasis.

Pathways

IL2R beta serves essential functions in the JAK-STAT and PI3K-AKT pathways. The binding of IL-2 to its receptor initiates a cascade of intracellular events which involve Janus kinases (JAKs) and signal transducer and activator of transcription (STATs) leading to transcriptional activation of various genes. Along with these pathways IL2R beta also interacts collaboratively with the gamma chain (γc) in promoting Downstream signaling events which enhance cell proliferation and survival.

Associated diseases and disorders

IL2R beta's function has implications in autoimmune diseases and certain types of leukemia. Alterations or dysregulation in IL-2R signaling pathways often play a role in autoimmune conditions due to its importance in maintaining immune tolerance. Moreover given IL-2 receptor's role in T-cell and NK-cell activity aberrations may contribute to uncontrolled cellular proliferation seen in leukemia. Understanding its interplay with the common gamma chain (CD132) can provide insights into therapeutic interventions targeting these disorders.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

7 product images

  • Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The antibody recognizes the full length and the intracellular fragment with molecular weights of 75KDa and 37KDa. (PMID: 20495002).

    Negative control: Daudi (PMID: 25486870).

    Exposure time: 59 seconds.

    All lanes: Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

    Lane 2: Daudi (human burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 61 kDa

    Observed band size: 37 kDa, 75 kDa

  • Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    IL2 Receptor beta/p75 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 μg with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HDLM-2 whole cell lysate 10 μg

    Lane 2: Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 IP in HDLM-2 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 in HDLM-2 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 84 seconds.

    All lanes: Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040)

    Predicted band size: 61 kDa

    Observed band size: 37 kDa, 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling IL2 Receptor beta/p75 with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human spleen is observed. The section was incubated with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling IL2 Receptor beta/p75 with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control: No staining in human cerebrum. The section was incubated with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    The molecular weight observed is consistent with what has been described in the literature (PMID: 20495002).

    Exposure time: 92 seconds.

    All lanes: Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040) at 1/1000 dilution

    Lane 1: HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

    Lane 2: HDLM-2 whole cell lysate treated with PNGase F at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/50000 dilution

    Predicted band size: 61 kDa

    Observed band size: 37 kDa, 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling IL2 Receptor beta/p75 with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human endometrium is observed. The section was incubated with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (ab282283)

    This data was developed using Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labelling IL2 Receptor beta/p75 with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human non-Hodgkin's lymphoma. The section was incubated with Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

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Product protocols

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