AB282283

Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free

BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IL2 Receptor beta/p75 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

View Alternative Names

CD122, IL15RB, IL2RB, Interleukin-2 receptor subunit beta, IL-2 receptor subunit beta, IL-2R subunit beta, IL-2RB, High affinity IL-2 receptor subunit beta, Interleukin-15 receptor subunit beta, p70-75, p75

7 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Negative control : No staining in human cerebrum. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human endometrium is observed. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human spleen tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on scattered immune cells of human spleen is observed. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded human non-Hodgkin's lymphoma tissue labelling IL2 Receptor beta/p75 with ab271040 at 1/100 (4.78 μg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Cytoplasmic staining on human non-Hodgkin's lymphoma. The section was incubated with ab271040 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Supplier Data

Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

IL2 Receptor beta/p75 was immunoprecipitated from 0.35 mg HDLM-2 (human Hodgkin lymphoma) whole cell lysate 10 μg with ab271040 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab271040 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.

Lane 1 : HDLM-2 whole cell lysate 10 μg

Lane 2 : ab271040 IP in HDLM-2 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab271040 in HDLM-2 whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 84 seconds.

All lanes:

Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (<a href='/en-us/products/primary-antibodies/il2-receptor-beta-p75-antibody-epr24336-29-ab271040'>ab271040</a>)

Predicted band size: 61 kDa

Observed band size: 37 kDa,75 kDa

false

Lab

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The antibody recognizes the full length and the intracellular fragment with molecular weights of 75KDa and 37KDa. (PMID : 20495002).

Negative control : Daudi (PMID : 25486870).

Exposure time : 59 seconds.

All lanes:

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (<a href='/en-us/products/primary-antibodies/il2-receptor-beta-p75-antibody-epr24336-29-ab271040'>ab271040</a>) at 1/1000 dilution

Lane 1:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

Lane 2:

Daudi (human burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 37 kDa,75 kDa

false

Lab

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

This data was developed using ab271040, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The molecular weight observed is consistent with what has been described in the literature (PMID : 20495002).

Exposure time : 92 seconds.

All lanes:

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] (<a href='/en-us/products/primary-antibodies/il2-receptor-beta-p75-antibody-epr24336-29-ab271040'>ab271040</a>) at 1/1000 dilution

Lane 1:

HDLM-2 (human Hodgkin lymphoma) whole cell lysate at 20 µg

Lane 2:

HDLM-2 whole cell lysate treated with PNGase F at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 37 kDa,75 kDa

false

Unconjugated

Anti-IL2 Receptor beta/p75 antibody [EPR24336-29]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24336-29

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab282283 is the carrier-free version of ab271040.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The IL2 Receptor beta also known as IL-2R beta IL2R beta or CD122/IL2RB protein is a component of the interleukin-2 receptor complex. It is part of the receptor system for the cytokine IL-2. This receptor subunit has a molecular mass of approximately 75 kDa and gets alternatively referred to as IL2R beta or p75. It is expressed on the surface of activated T cells natural killer (NK) cells and in some cases myeloid lineage cells. This expression pattern correlates IL2R beta's role in essential immune functions and signaling processes.

Biological function summary

IL2R beta plays a critical role in cytokine binding and signal transduction for the interleukin-2 receptor which is a heterodimeric complex. This complex consists of the IL-2 receptor alpha (CD25) IL-2 receptor beta and a common gamma chain (γc CD132). The presence of IL-2R beta allows the receptor to mediate biological processes like cell proliferation differentiation and survival particularly within the immune system. The extent to how IL2R beta integrates into these processes makes it an important player in immune regulation and homeostasis.

Pathways

IL2R beta serves essential functions in the JAK-STAT and PI3K-AKT pathways. The binding of IL-2 to its receptor initiates a cascade of intracellular events which involve Janus kinases (JAKs) and signal transducer and activator of transcription (STATs) leading to transcriptional activation of various genes. Along with these pathways IL2R beta also interacts collaboratively with the gamma chain (γc) in promoting Downstream signaling events which enhance cell proliferation and survival.

IL2R beta's function has implications in autoimmune diseases and certain types of leukemia. Alterations or dysregulation in IL-2R signaling pathways often play a role in autoimmune conditions due to its importance in maintaining immune tolerance. Moreover given IL-2 receptor's role in T-cell and NK-cell activity aberrations may contribute to uncontrolled cellular proliferation seen in leukemia. Understanding its interplay with the common gamma chain (CD132) can provide insights into therapeutic interventions targeting these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. This beta subunit is involved in receptor mediated endocytosis and transduces the mitogenic signals of IL2. Probably in association with IL15RA, involved in the stimulation of neutrophil phagocytosis by IL15 (PubMed : 15123770, PubMed : 31040185).

See full target information IL2RB

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Immunoprecipitation - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Western blot - Anti-IL2 Receptor beta/p75 antibody [EPR24336-29] - BSA and Azide free (AB282283)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise