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AB314887

Anti-IL22 RA2/IL-22BP antibody [EPR25046-231]

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Rabbit Recombinant Monoclonal IL22 RA2/IL-22BP antibody. Suitable for WB and reacts with Human samples.

View Alternative Names

UNQ5793/PRO19598/PRO19822, IL22RA2, Interleukin-22 receptor subunit alpha-2, IL-22 receptor subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Cytokine receptor class-II member 10, Cytokine receptor family 2 member 10, Interleukin-22-binding protein, ZcytoR16, CRF2-10, CRF2-S1, IL-22BP, IL22BP

3 Images
Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)
  • WB

Supplier Data

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)

Blocking and diluting buffer and concentration : 5% NFDM/TBST,

Low expression : colon cancer (PMID : 32585307).

The identity of the bands between 40 kDa and 250 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (ab314887) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human colon cancer tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 35 kDa

false

Exposure time: 180s

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)
  • WB

Supplier Data

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of IL22RA2 is upregulated in response to PMA treatment (PMID : 11481447, 33178031).

The identity of the band at 40 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (ab314887) at 1/1000 dilution

Lane 1:

Untreated U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

U937 treated with 200nM PMA for 72 hours whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa,36 kDa

false

Exposure time: 180s

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)
  • WB

Supplier Data

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (AB314887)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The expression of IL22RA2 is upregulated in response to PMA treatment (PMID : 11481447, 33178031).

The identity of the band at 40 kDa is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] (ab314887) at 1/1000 dilution

Lane 1:

Untreated U937 (human histiocytic lymphoma monocyte) whole cell lysate at 20 µg

Lane 2:

U937 treated with 200nM PMA for 72 hours, then transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 3:

U937 treated with 200nM PMA for 72 hours, then transfected with siRNA specifically targeting IL22 RA2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 35 kDa

false

Exposure time: 81s

  • Carrier free

    Anti-IL22 RA2/IL-22BP antibody [EPR25046-231] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25046-231

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Complementary Products

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Sandwich ELISA - Human IL-22RA2 ELISA kit (IL-22BP) (AB267601)

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Primary Antibodies

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Secondary Antibodies

AB150077

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Primary Antibodies

AB245076

Anti-IL-28A antibody [EPR20974-48] - BSA and Azide free (Detector)

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  • 0
  • 0
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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL22 RA2 also known as IL-22BP (IL-22 binding protein) acts as a soluble decoy receptor for IL-22 and it plays a role in modulating IL-22 activity. IL-22 promotes the survival and innate immunity of epithelial cells. IL22 RA2 binds IL-22 with high affinity preventing it from interacting with its membrane receptor IL22R1. This protein is approximately 27 kDa in size and is expressed mainly in the placenta spleen and skin. IL22 RA2 does not get anchored to the cell membrane which allows it to regulate IL-22 activity distantly from the site of its production.
Biological function summary

IL22 RA2 is involved in dampening the pro-inflammatory effects of IL-22. IL-22 is part of the interleukin-10 (IL-10) cytokine family and IL22 RA2 adjusts the balance of IL-22 activity within tissue microenvironments. By neutralizing IL-22 IL22 RA2 reduces cytokine signaling that would lead to excessive inflammation. Though it does not form part of a larger receptor complex on the cell surface its role is significant in controlling the spread of IL-22 mediated signals in tissues prone to inflammation.

Pathways

IL22 RA2 influences the IL-22/STAT3 signaling pathway which is central in managing inflammatory responses and maintaining epithelial barrier functions. IL22 RA2 inhibits IL-22 induced STAT3 phosphorylation effectively blocking downstream pathway activation. Another related protein in this signaling route is IL22R1 which when not bound by IL22 RA2 forms a receptor complex with IL10R2 to mediate the effects of IL-22 in target tissues.

IL22 RA2 has been linked to inflammatory bowel disease (IBD) and psoriasis. These conditions show deregulation of IL-22 activity where IL22 RA2 plays a role in modulating the inflammatory environment. By binding IL-22 IL22 RA2 affects the IL-22/IL22R1 axis reducing exacerbation of chronic inflammation seen in these diseases. Understanding its interaction with IL-22 can aid in the development of targeted therapies for managing inflammation in conditions such as IBD and psoriasis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.. Isoform 1 may play a role in establishing and maintaining successful pregnancy.
See full target information IL22RA2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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