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AB324945

Anti-IL22 RA2/IL-22BP antibody [EPR27285-7]

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Rabbit Recombinant Monoclonal IL22 RA2/IL-22BP antibody. Suitable for Flow Cyt (Intra) and reacts with Mouse samples.

View Alternative Names

Interleukin-22 receptor subunit alpha-2, IL-22 receptor subunit alpha-2, IL-22R-alpha-2, IL-22RA2, Interleukin-22-binding protein, ZcytoR16, CRF2-S1, IL-22BP, IL22BP

2 Images
Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized WEHI-231 (mouse B cell lymphoma B lymphocyte, Left) / DCs (mouse dendritic cell sarcoma cell, Right) cells labelling IL22 RA2/IL-22BP with ab324945 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Low expression : WEHI-231 (PMID : 23653115).

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-IL22 RA2/IL-22BP antibody [EPR27285-7] (AB324945)

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days ((Lower left and right) Mouse bone marrow (Upper left and right)) cells labelling IL22 RA2/IL-22BP with ab324945 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Cells were co-stained with anti-CD11c conjugated to BV421 and anti-MHCII conjugated to APC/CY7, then fixed with 2% PFA for 10 min followed by intracellular staining.
Gated on viable CD11c positive cells.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR27285-7

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/500", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro). May play an important role as an IL22 antagonist in the regulation of inflammatory responses.
See full target information Il22ra2
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com