AB220359

Anti-IL27RA/WSX-1 antibody [EPR20863-3]

RabMAb
Recombinant
What is this?

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(4 Publications)

Rabbit Recombinant Monoclonal WSX-1 antibody. Suitable for WB and reacts with Mouse samples. Cited in 4 publications.

View Alternative Names

Tccr, Wsx1, Il27ra, Interleukin-27 receptor subunit alpha, IL-27 receptor subunit alpha, IL-27R subunit alpha, IL-27R-alpha, IL-27RA, Type I T-cell cytokine receptor, WSX-1, TCCR

3 Images

Lab

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Data from collaborator.

Exposure time : 1 minute.

WSX-1 = IL27Ra

All lanes:

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution

Lane 1:

Wild-type mouse spleen (1) tissue lysate at 20 µg

Lane 2:

Wild-type mouse spleen (2) tissue lysate at 20 µg

Lane 3:

Wild-type mouse spleen (3) tissue lysate at 20 µg

Lane 4:

IL27Ra knockout mouse spleen (1) tissue lysate at 20 µg

Lane 5:

IL27Ra knockout mouse spleen (2) tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 110-120 kDa

false

Lab

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Data from collaborator.

Exposure time : 1 minute.

WSX-1 = IL27Ra

All lanes:

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (ab220359) at 1/1000 dilution

Lane 1:

Wild-type mouse bone marrow (1) tissue lysate at 20 µg

Lane 2:

Wild-type mouse bone marrow (2) tissue lysate at 20 µg

Lane 3:

Wild-type mouse bone marrow (3) tissue lysate at 20 µg

Lane 4:

IL27Ra knockout mouse bone marrow (1) tissue lysate at 20 µg

Lane 5:

IL27Ra knockout mouse bone marrow (2) tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size: 69 kDa

Observed band size: 110-120 kDa

false

Lab

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (ab220359) at 1/5000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate unboiled at 15 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) transfected with Mouse WSX-1 expression vector containing a myc-His-tag®, whole cell lysate unboiled at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 69 kDa

Observed band size: 100 kDa

false

Exposure time: 3s

Carrier free

Anti-IL27RA/WSX-1 antibody [EPR20863-3] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20863-3

Isotype

IgG

Carrier free

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The WSX-1 protein also known as IL-27Rα or TCCR is a subunit of the interleukin-27 receptor. It weighs approximately 74 kDa and is mainly expressed on various immune cells including T cells B cells and monocytes. WSX-1 engages in the immune signaling by interacting specifically with its ligand IL-27 to drive immunological responses. This receptor belongs to the type I cytokine receptor family and is structurally characterized by certain conserved motifs important for its signaling functions.

Biological function summary

WSX-1 plays a role in regulating immune cell activity and differentiation. It acts as a part of a receptor complex with gp130 to form the IL-27 receptor which transmits signals that modulate the activity of T helper cells. This signaling influences the production of pro-inflammatory and anti-inflammatory cytokines impacting immune system balance. The function of WSX-1 remains critical for controlling inflammatory responses and ensuring immune tolerance.

Pathways

WSX-1 contributes to the JAK/STAT signaling pathway and influences the differentiation of Th1 cells. The connection to JAK/STAT signaling involves various proteins such as STAT1 and STAT3 which transduce IL-27-induced signals leading to gene expression changes in immune cells. Through its action in these pathways WSX-1 regulates immune responses and can influence inflammation dynamics in various biological contexts.

WSX-1 has associations with autoimmune diseases and infectious conditions. Alterations in its signaling pathways can lead to dysregulated immune responses contributing to diseases like multiple sclerosis and inflammatory bowel disease. The protein’s interaction with IL-27 is central in these conditions as it influences the pro-inflammatory and regulatory functions of the immune system. Furthermore studies suggest a link between WSX-1 activity and the modulation of responses against infections linking it to potential therapeutic targets for managing related disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for IL27. Requires IL6ST/GP130 to mediate signal transduction in response to IL27. This signaling system acts through STAT3 and STAT1. Involved in the regulation of Th1-type immune responses. Also appears to be involved in innate defense mechanisms.

See full target information Il27ra

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature 600:314-318 PubMed34819664

2021

IL-27 signalling promotes adipocyte thermogenesis and energy expenditure.

Applications

Species

Mouse

Qian Wang,Dehai Li,Guangchao Cao,Qiping Shi,Jing Zhu,Mingyue Zhang,Hao Cheng,Qiong Wen,Hao Xu,Leqing Zhu,Hua Zhang,Rachel J Perry,Olga Spadaro,Yunfan Yang,Shengqi He,Yong Chen,Baocheng Wang,Guangqiang Li,Zonghua Liu,Caixian Yang,Xiaoli Wu,Libing Zhou,Qinghua Zhou,Zhenyu Ju,Hongyun Lu,Yongjie Xin,Xiaoyong Yang,Cunchuan Wang,Yong Liu,Gerald I Shulman,Vishwa Deep Dixit,Ligong Lu,Hengwen Yang,Richard A Flavell,Zhinan Yin

Science advances 7: PubMed33863728

2021

SRSF1 serves as a critical posttranscriptional regulator at the late stage of thymocyte development.

Applications

Species

Mouse

Zhihong Qi,Fang Wang,Guotao Yu,Di Wang,Yingpeng Yao,Menghao You,Jingjing Liu,Juanjuan Liu,Zhen Sun,Ce Ji,Yuanchao Xue,Shuyang Yu

Blood 136:183-198 PubMed32305041

2020

Aging-induced IL27Ra signaling impairs hematopoietic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Hanqing He,Panglian Xu,Xiaofei Zhang,Min Liao,Qiongye Dong,Tingting Cong,Baixue Tang,Xiuxiu Yang,Maoqing Ye,Yingjun Chang,Weihua Liu,Xiaowo Wang,Zhenyu Ju,Jianwei Wang

Experimental and therapeutic medicine 16:1095-1102 PubMed30112052

2018

Silence of IGFBP7 suppresses apoptosis and epithelial mesenchymal transformation of high glucose induced-podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojun Cai,Lei Wang,Xuling Wang,Fengyan Hou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL27RA/WSX-1 antibody [EPR20863-3]

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Western blot - Anti-IL27RA/WSX-1 antibody [EPR20863-3] (AB220359)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (4)

Product promise