AB281019

Anti-IL2RA antibody [EPR23862-65] - BSA and Azide free (Detector)

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal IL2RA antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

CD25, Interleukin-2 receptor subunit alpha, IL-2 receptor subunit alpha, IL-2-RA, IL-2R subunit alpha, IL2-RA, TAC antigen, p55, IL2RA

1 Images

Sandwich ELISA - Anti-IL2RA antibody [EPR23862-65] - BSA and Azide free (Detector) (AB281019)

sELISA

Supplier Data

Sandwich ELISA - Anti-IL2RA antibody [EPR23862-65] - BSA and Azide free (Detector) (AB281019)

Example of Human IL-2 Receptor alpha standard curve. Background-subtracted data values (mean +/- SD) are graphed.

Biotin

Biotin Anti-IL2RA antibody [EPR23862-65] (Detector)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23862-65

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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Product details

ab281019 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human IL2RA. The recommended pair for sandwich ELISA is:
Capture: ab281169, Human IL2RA Capture Antibody (unconjugated)
Detector: ab281019, Human IL2RA Detector Antibody (unconjugated)
The reference range value is 15.625 - 2000 pg/ml.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL2RA also known as CD25 or interleukin-2 receptor alpha chain is a protein that performs an important role in the immune system. It has a molecular mass of approximately 55 kDa. This protein is expressed mainly by activated T cells regulatory T cells and recent thymic emigrants. IL2RA forms an important part of the high-affinity IL-2 receptor complex which also includes the beta and gamma chains. The presence of IL2RA ensures effective signaling and response to interleukin-2 a cytokine that promotes the proliferation and differentiation of immune cells.

Biological function summary

IL2RA engages in modulating the immune response as it acts in the high-affinity interleukin-2 receptor complex. It enables the IL-2 signaling that supports the development and expansion of T cells including regulatory T cells which help in maintaining immune tolerance. This receptor plays a fundamental role in T-cell fostered adaptive immunity facilitating the communication between cells that regulate immune responses. By regulating these processes IL2RA maintains balance in the immune system and prevents overactivation.

Pathways

Researchers recognize IL2RA as an essential participant in the JAK-STAT signaling pathway and the MAPK pathway. These pathways promote cellular responses such as growth proliferation and apoptosis which are important for immune function and homeostasis. IL2RA initiates signaling cascades through interaction with the interleukin-2 receptor beta (CD122) and common gamma chain (CD132) illustrating its direct link with these related proteins that manage immune response activities.

IL2RA has implications in autoimmune diseases like multiple sclerosis and type 1 diabetes where immune cell regulation becomes dysregulated. Dysregulation or mutations in IL2RA can impair its ability to modulate immune responses thereby leading to such autoimmune conditions. Furthermore IL2RA's interaction with other proteins like CD122 and CD132 hints at potential therapeutic targets as these proteins play roles in the disease pathways affecting immune system balance and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for interleukin-2. The receptor is involved in the regulation of immune tolerance by controlling regulatory T cells (TREGs) activity. TREGs suppress the activation and expansion of autoreactive T-cells.

See full target information IL2RA

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL2RA antibody [EPR23862-65] - BSA and Azide free (Detector)

Sandwich ELISA - Anti-IL2RA antibody [EPR23862-65] - BSA and Azide free (Detector) (AB281019)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

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Primary Antibodies

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Product promise