Rabbit Recombinant Monoclonal IL36 alpha/IL-1F6 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Images
![Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunoprecipitation-img150168.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93509.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118855.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85467.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92845.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (AB270608)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | IHC-P | ICC | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ICC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells linked to a pro-inflammatory response. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1, and the production of pro-inflammatory cytokines such as TNF-alpha, IL-8 and IL-6. In cultured monocytes up-regulates expression of IL-1A, IL-1B and IL-6. In myeloid dendritic cells involved in cell maturation by up-regulating surface expression of CD83, CD86 and HLA-DR. In monocyte-derived dendritic cells facilitates dendritic cell maturation and drives T-cell proliferation. May play a role in pro-inflammatory effects in the lung.
Alternative names
FIL1E, IL1E, IL1F6, IL36A, Interleukin-36 alpha, FIL1 epsilon, Interleukin-1 epsilon, Interleukin-1 family member 6, IL-1 epsilon, IL-1F6
Recommended products
Rabbit Recombinant Monoclonal IL36 alpha/IL-1F6 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23089-87
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab270608 is the carrier-free version of Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
IL-36 alpha also known as IL-1F6 is a member of the IL-1 cytokine family and has a molecular mass of approximately 18 kDa. It is mostly expressed in epithelial tissues like the skin and lungs where it plays a role in the immune response. This protein acts as a pro-inflammatory mediator by binding to the IL-36 receptor stimulating the production of various cytokines and promoting inflammation.
- Biological function summary
IL-36 alpha is involved in the activation of immune cells including macrophages and keratinocytes which further boosts the inflammatory response. It is not part of a larger complex but interacts with specific receptors on cell surfaces to exert its functions. This cytokine is known to enhance the expression of other inflammatory mediators which can amplify immune responses in the local tissue environment.
- Pathways
IL-36 alpha takes part in the IL-1 signaling pathway and the NF-kB signaling pathway which are important for the regulation of immune and inflammatory responses. Through these pathways IL-36 alpha interacts with other proteins including IL-36 receptor antagonists and IL-37 to manage inflammation and regulate immune cell behavior. These pathways help in coordinating defense mechanisms under inflammatory conditions.
- Associated diseases and disorders
IL-36 alpha has been linked to psoriasis and inflammatory bowel disease conditions that arise due to dysregulated immune responses. IL-36 alpha interacts with other proteins like IL-17 and TNF-alpha which are also involved in the pathophysiology of these diseases. Understanding its role in these processes may offer insights into potential therapeutic targets for treating such inflammatory disorders.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunoprecipitation-img150168.jpg "Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)")
Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)
IL36 alpha/IL-1F6 was immunoprecipitated from 0.35 mg human tonsil tissue lysate with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/30. Western blot was performed on the immunoprecipitate using Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.
Lane 1: Human tonsil tissue lysate.
Lane 2: Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 IP in human tonsil tissue lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 in human tonsil tissue lysate.
Blocking and dilution buffer and concentration/ 5% NFDM/TBST.
Exposure time: 24 secs.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271).
All lanes: Immunoprecipitation - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271)
Predicted band size: 18 kDa
Observed band size: 17 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img93509.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)
Immunohistochemical analysis of paraffin-embedded human psoriatic skin tissue labeling IL36 alpha/IL-1F6 with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human psoriatic skin is observed (PMID: 28043870). The section was incubated with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img118855.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)
Immunohistochemical analysis of paraffin-embedded human skin tissue labeling IL36 alpha/IL-1F6 with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Negative control: No staining on human healthy skin (PMID: 28043870).
The section was incubated with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img85467.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL36 alpha/IL-1F6 with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on tonsil epithelium is observed. The section was incubated with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608), expandable thumbnail](https://content.abcam.com/products/images/il36-alpha-il-1f6-antibody-epr23089-87-bsa-and-azide-free-ab270608--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img92845.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] - BSA and Azide free (ab270608)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue labeling IL36 alpha/IL-1F6 with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 at 1/2000 followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human colon carcinoma tissue is observed (PMID: 30452921). The section was incubated with Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-IL36 alpha/IL-1F6 antibody [EPR23089-87] ab269271).
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