AB156783

Anti-IL36 gamma/IL-1F9 antibody [OTI2F4]

Name: Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) | Abcam
Brand: Abcam Limited
SKU: AB156783
Price: 400 USD
Availability: InStock

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(16 Publications)

Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) is a mouse monoclonal antibody detecting IL36 gamma/IL-1F9 in Western Blot, IHC-P, ICC/IF. Suitable for Human.

- Over 10 publications

View Alternative Names

IL1E, IL1F9, IL1H1, IL1RP2, UNQ2456/PRO5737, IL36G, Interleukin-36 gamma, IL-1-related protein 2, Interleukin-1 epsilon, Interleukin-1 family member 9, Interleukin-1 homolog 1, IL-1RP2, IL-1 epsilon, IL-1F9, IL-1H1

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Immunohistochemical analysis of paraffin-embedded Human colon adenocarcinoma tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IL36 gamma/IL-1F9 with ab156783 at 1/150 dilution

Immunocytochemistry/ Immunofluorescence - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Immunofluorescent analysis of HeLa cells labeling IL36 gamma/IL-1F9 using ab156783 at 1/100 dilution.

Unknown

Western blot - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

HEK293T cell lysates were generated from transient transfection of the cDNA clone (RC213525)

All lanes:

Western blot - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) at 1/4000 dilution

Lane 1:

HEK293T cells transfected with pCMV6-ENTRY control at 5 µg

Lane 2:

HEK293T cells transfected with pCMV6-ENTRY IL36 gamma/IL-1F9 cDNA at 5 µg

Predicted band size: 19 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2F4

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human IL36G.

Q9NZH8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "Perform heat-induced epitope retrieval with 10mM citric buffer, pH 6.0 at 120oC for 3 minutes.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" } } }

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Product details

What is this antibody validated in?
Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (ab156783) is a mouse monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human samples.

What is the molecular weight of IL36 gamma/IL-1F9?
Anti-IL36 gamma/IL-1F9 [OTI2F4] (ab156783) specifically detects a band for IL36 gamma/IL-1F9 (UniProt: Q9NZH8) at a molecular weight of 19kDa.

Trusted by the scientific community
Anti-IL36 gamma/IL-1F9 [OTI2F4] (ab156783) was first used in a scientific publication in 2013 and has been cited over 10 times in peer-reviewed journals.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

ab156783 is purified from TCS by affinity chromatography

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL36 gamma also known as IL-1F9 or IL-36 is a member of the interleukin-1 cytokine family. The protein weighs approximately 22 kDa. It is expressed highly in epithelial tissues such as keratinocytes in the skin bronchial tissues and intestinal lining. It acts mainly on nearby cells to influence immune responses through cytokine signaling.

Biological function summary

IL36 gamma plays a role in the immune and inflammatory responses. It operates as a cytokine that signals through the IL-1 receptor family leading to the activation of NF-kB and MAPK pathways. This member of the IL-1 family does not form a complex by itself but interacts with IL-36R to exert its functions. The protein influences processes such as cell proliferation differentiation and the regulation of immune responses to external pathogens or injuries.

Pathways

IL36 gamma is involved in inflammatory and immune signaling pathways. Within the immune response pathway it stimulates the production of other cytokines and pro-inflammatory molecules. IL36 gamma is associated with the IL-1 signaling pathway alongside other cytokines such as IL-1 and IL-18 which all contribute to immune modulation and inflammatory responses.

IL36 gamma has connections to psoriasis and inflammatory bowel disease. In psoriasis increased levels of IL36 gamma correlate with heightened skin inflammation and proliferation of keratinocytes. In inflammatory bowel disease IL36 gamma contributes to the inflammatory state of the gut. The activities of IL36 gamma often link with other cytokines such as TNF-alpha which collectively drive the inflammatory processes observed in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cytokine that binds to and signals through the IL1RL2/IL-36R receptor which in turn activates NF-kappa-B and MAPK signaling pathways in target cells. Part of the IL-36 signaling system that is thought to be present in epithelial barriers and to take part in local inflammatory response; similar to the IL-1 system with which it shares the coreceptor IL1RAP. Seems to be involved in skin inflammatory response by acting on keratinocytes, dendritic cells and indirectly on T-cells to drive tissue infiltration, cell maturation and cell proliferation. In cultured keratinocytes induces the expression of macrophage, T-cell, and neutrophil chemokines, such as CCL3, CCL4, CCL5, CCL2, CCL17, CCL22, CL20, CCL5, CCL2, CCL17, CCL22, CXCL8, CCL20 and CXCL1; also stimulates its own expression and that of the prototypic cutaneous pro-inflammatory parameters TNF-alpha, S100A7/psoriasin and inducible NOS. May play a role in pro-inflammatory responses during particular neutrophilic airway inflammation : activates mitogen-activated protein kinases and NF-kappa B in primary lung fibroblasts, and stimulates the expression of IL-8 and CXCL3 and Th17 chemokine CCL20 in lung fibroblasts. May be involved in the innate immune response to fungal pathogens, such as Aspergillus fumigatus.

See full target information IL36G

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Publications (16)

Recent publications for all applications. Explore the full list and refine your search

Experimental dermatology 32:1915-1923 PubMed37724787

2023

Characterisation of IL-1 family members in Sweet syndrome highlights the overexpression of IL-1β and IL-1R3 as possible therapeutic targets.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Calabrese,Francesca Ney,Rui Aoki,Chiara Moltrasio,Angelo V Marzano,Katrin Kerl,Pia-Charlotte Stadler,Takashi K Satoh,Lars E French

The Journal of dermatology 50:1150-1155 PubMed37288510

2023

Three cases of subcorneal pustular dermatosis with immunohistochemical examinations.

Applications

Unspecified application

Species

Unspecified reactive species

Megumi Kishimoto,Mayumi Komine,Hirofumi Okada,Atsuko Sato,Koji Kamiya,Takeo Maekawa,Satoru Murata,Mamitaro Ohtsuki

Journal of lipid research 63:100308 PubMed36332686

2022

Ceramide Analysis in Combination With Genetic Testing May Provide a Precise Diagnosis for Self-Healing Collodion Babies.

Applications

Unspecified application

Species

Unspecified reactive species

Takuya Takeichi,Yusuke Ohno,Kana Tanahashi,Yasutoshi Ito,Ken Shiraishi,Ryo Utsunomiya,Satoshi Yoshida,Kenta Ikeda,Hayato Nomura,Shin Morizane,Koji Sayama,Tomoo Ogi,Yoshinao Muro,Akio Kihara,Masashi Akiyama

JCI insight 7: PubMed35862195

2022

Proprotein convertase subtilisin/kexin type 9 is a psoriasis-susceptibility locus that is negatively related to IL36G.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Merleev,Antonio Ji-Xu,Atrin Toussi,Lam C Tsoi,Stephanie T Le,Guillaume Luxardi,Xianying Xing,Rachael Wasikowski,William Liakos,Marie-Charlotte Brüggen,James T Elder,Iannis E Adamopoulos,Yoshihiro Izumiya,Annie R Leal,Qinyuan Li,Nikolay Y Kuzminykh,Amanda Kirane,Alina I Marusina,Johann E Gudjonsson,Emanual Maverakis

Cell reports 33:108515 PubMed33326792

2020

The Proinflammatory Cytokine IL-36γ Is a Global Discriminator of Harmless Microbes and Invasive Pathogens within Epithelial Tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas Macleod,Joseph S Ainscough,Christina Hesse,Sebastian Konzok,Armin Braun,Anna-Lena Buhl,Joerg Wenzel,Paul Bowyer,Yutaka Terao,Sarah Herrick,Miriam Wittmann,Martin Stacey

Methods in molecular biology (Clifton, N.J.) 2248:155-165 PubMed33185874

2020

Experimental Methods for the Immunological Characterization of Paradoxical Psoriasis Reactions Induced by TNF-α Biologics.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Morelli,Claudia Scarponi,Stefania Madonna,Cristina Albanesi

Experimental dermatology 30:249-261 PubMed33067891

2020

Early transcriptional changes after UVB treatment in atopic dermatitis include inverse regulation of IL-36γ and IL-37.

Applications

Unspecified application

Species

Unspecified reactive species

Astrid H Lossius,Teresa L Berents,Frank Saetre,Hogne R Nilsen,Maria Bradley,Samina Asad,Guttorm Haraldsen,Olav Sundnes,Jan-Øivind Holm

BMC cancer 20:92 PubMed32013927

2020

IL-36 s in the colorectal cancer: is interleukin 36 good or bad for the development of colorectal cancer?

Applications

Unspecified application

Species

Unspecified reactive species

Feier Chen,Meng Qu,Feng Zhang,Zhenyu Tan,Qinghua Xia,Brett D Hambly,Shisan Bao,Kun Tao

The journal of pathology. Clinical research 6:55-68 PubMed31577850

2019

Paradoxical psoriasis induced by TNF-α blockade shows immunological features typical of the early phase of psoriasis development.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Fania,Martina Morelli,Claudia Scarponi,Laura Mercurio,Fernanda Scopelliti,Caterina Cattani,Giovanni Luca Scaglione,Tiziano Tonanzi,Maria Antonietta Pilla,Gianluca Pagnanelli,Cinzia Mazzanti,Giampiero Girolomoni,Andrea Cavani,Stefania Madonna,Cristina Albanesi

Journal of dermatological science 94:244-251 PubMed31010609

2019

Cyto/chemokine profile of in vitro scratched keratinocyte model: Implications of significant upregulation of CCL20, CXCL8 and IL36G in Koebner phenomenon.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhisa Furue,Takamichi Ito,Yuka Tanaka,Ayako Yumine,Akiko Hashimoto-Hachiya,Masaki Takemura,Maho Murata,Kazuhiko Yamamura,Gaku Tsuji,Masutaka Furue

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-IL36 gamma/IL-1F9 antibody [OTI2F4]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Immunocytochemistry/ Immunofluorescence - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Western blot - Anti-IL36 gamma/IL-1F9 antibody [OTI2F4] (AB156783)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (16)

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