AB200687

Anti-IL3RA/CD123 antibody

Name: Anti-IL3RA/CD123 antibody (ab200687) | Abcam
Brand: Abcam Limited
SKU: AB200687
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal IL3RA/CD123 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IL3RA aa 250-350.

View Alternative Names

CD123, IL3R, IL3RA, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA

1 Images

Supplier Data

Western blot - Anti-IL3RA/CD123 antibody (AB200687)

All lanes:

Western blot - Anti-IL3RA/CD123 antibody (ab200687)

Lane 1:

MCF7 whole cell lysate

Lane 2:

NIH3T3 whole cell lysate

Lane 3:

PC12 whole cell lysate

Predicted band size: 43 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human IL3RA aa 250-350. The exact immunogen used to generate this antibody is proprietary information.

P26951

Specificity

ab200687 detects endogenous levels of IL3RA / CD123 protein.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

purified by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.2 Preservative: 0.0975% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IL3RA also known as CD123 or interleukin-3 receptor alpha chain is a transmembrane protein with a molecular weight of approximately 70-80 kDa. This protein frequently expresses on the surface of hematopoietic cells particularly those of myeloid lineage including mast cells and basophils. Researchers use CD123 as a marker for identifying certain cell types in flow cytometry. Antibodies such as anti-CD123 are available for various applications including identifying these cells in research and clinical settings.

Biological function summary

IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.

Pathways

IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.

IL3RA expression relates closely to certain hematological conditions such as acute myeloid leukemia (AML) and systemic mastocytosis. In AML increased expression of CD123 marks leukemic cells making it a target for therapeutic intervention. The interaction of IL3RA with FLT3 another tyrosine kinase is of particular interest in these disorders as both proteins contribute to the proliferation of malignant cells through related signaling pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cell surface receptor for IL3 expressed on hematopoietic progenitor cells, monocytes and B-lymphocytes that controls the production and differentiation of hematopoietic progenitor cells into lineage-restricted cells (PubMed : 10527461). Ligand stimulation rapidly induces hetrodimerization with IL3RB, phosphorylation and enzyme activity of effector proteins such as JAK2 and PI3K that play a role in signaling cell proliferation and differentiation. Activation of JAK2 leads to STAT5-mediated transcriptional program (By similarity).

See full target information IL3RA

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Thyroid research 17:22 PubMed39491028

2024

Losartan is more effective than angiotensin (1-7) in preventing thyroxine-induced renal injury in the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Slava Malatiali,Mabayoje Oriowo

RNA biology 17:784-793 PubMed32174258

2020

LncRNA MAGI2-AS3 inhibits the self-renewal of leukaemic stem cells by promoting TET2-dependent DNA demethylation of the LRIG1 promoter in acute myeloid leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Lijuan Chen,Xu Fan,Jianhua Zhu,Xuexin Chen,Yiling Liu,Hao Zhou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-IL3RA/CD123 antibody

Western blot - Anti-IL3RA/CD123 antibody (AB200687)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise