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Rabbit Recombinant Monoclonal IL3RA/CD123 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL3RA aa 1-200.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (AB257307), expandable thumbnail
  • Protein Array - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (AB257307), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • Recombinant Fragment Protein within Human IL3RA aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P26951

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Protein ArrayIHC-P
Human
Tested
Tested

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

0.25000-0.50000 µg/mL

Notes

Incubate with primary antibody for 30 minutes at RT.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

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Target data

Function

This is a receptor for interleukin-3.

Alternative names

Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, IL3RA, IL3R

Recommended products

Rabbit Recombinant Monoclonal IL3RA/CD123 antibody. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human IL3RA aa 1-200.

Alternative names

Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, IL3RA, IL3R

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • Recombinant Fragment Protein within Human IL3RA aa 1-200. The exact immunogen used to generate this antibody is proprietary information. Database link P26951
Clone number

IL3RA/2947R

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from bioreactor concentrate.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

IL3RA also known as CD123 or interleukin-3 receptor alpha chain is a transmembrane protein with a molecular weight of approximately 70-80 kDa. This protein frequently expresses on the surface of hematopoietic cells particularly those of myeloid lineage including mast cells and basophils. Researchers use CD123 as a marker for identifying certain cell types in flow cytometry. Antibodies such as anti-CD123 are available for various applications including identifying these cells in research and clinical settings.

Biological function summary

IL3RA forms part of a heterodimeric receptor complex binding with the beta-common (βc) chain to create the high-affinity IL-3 receptor. This complex plays a role in mediating the effects of interleukin-3 a cytokine that promotes the growth and differentiation of pluripotent hematopoietic stem cells. Through this mechanism the receptor influences immune response modulation particularly in allergic reactions and inflammation.

Pathways

IL3RA interacts with the JAK/STAT pathway which is essential for signal transduction and transcription activation in response to IL-3 binding. This pathway involves proteins like Janus kinase 2 (JAK2) and Signal Transducer and Activator of Transcription 5 (STAT5) which transmit signals from IL3RA engagement to the cell nucleus promoting gene expression associated with cell proliferation and survival.

Associated diseases and disorders

IL3RA expression relates closely to certain hematological conditions such as acute myeloid leukemia (AML) and systemic mastocytosis. In AML increased expression of CD123 marks leukemic cells making it a target for therapeutic intervention. The interaction of IL3RA with FLT3 another tyrosine kinase is of particular interest in these disorders as both proteins contribute to the proliferation of malignant cells through related signaling pathways.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (ab257307), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (ab257307)

    Formalin-fixed, paraffin-embedded human tonsil tissue labeling IL3RA/CD123 with ab257307 at 0.5 μg/ml in immunohistochemical analysis.

    Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes.

  • Protein Array - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (ab257307), expandable thumbnail

    Protein Array - Anti-IL3RA/CD123 antibody [IL3RA/2947R] (ab257307)

    Protein array containing >19,000 full-length human proteins, probed with ab257307.

    Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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