Skip to main content

Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...
Loading...

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow Cyt
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell line - Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell line - Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Transfected cell line - Human

Dilution info

1/4000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Mouse

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell line - Human

Dilution info

-

Notes

-

Target data

Function

Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity).

Alternative names

Recommended products

  1. Loading...
  2. Loading...
  3. Loading...
  4. Loading...

Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR25269-115

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag. labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positivie staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temerature.

    The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101). 

    Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag, labeling IL-4 with ab300138 at 1/4000 dilution (0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temperature. 

    The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101). 

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

There was a problem

We can’t download that datasheet. Please try again. If you need help, contact our Customer Services team at technical@abcam.com