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AB300138

Anti-IL4 antibody [EPR25269-115]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

5

(1 Review)

|

(2 Publications)

Rabbit Recombinant Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 2 publications.

View Alternative Names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded : Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B : HEK-293T cells transfected with empty vector containing a his tag labeling IL-4 with ab300138 at 1/4000 dilution (0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temperature.

The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody only control : Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101).  Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138)

Immunohistochemical analysis of paraffin-embedded : Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B : HEK-293T cells transfected with empty vector containing a his tag, labeling IL-4 with ab300138 at 1/4000 dilution (0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temperature.  The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.  Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Secondary antibody only control : used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).

  • Carrier free

    Anti-IL4 antibody [EPR25269-115] (BSA and Azide free)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR25269-115

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed : 1993171, PubMed : 3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed : 1993171). Also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed : 2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is also expressed on nonhematopoietic cells (PubMed : 10219247, PubMed : 11526337, PubMed : 18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed : 7721895).
See full target information IL4
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Theranostics 14:3014-3028 PubMed38773979

2024

Cancer-associated fibroblast-derived periostin promotes papillary thyroid tumor growth through integrin-FAK-STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorui Jin,Qianmei Deng,Shuting Ye,Shuting Liu,Yilong Fu,Yingfu Liu,Guoyang Wu,Gaoliang Ouyang,Tiantian Wu

Cancer cell international 23:227 PubMed37779195

2023

HK3 stimulates immune cell infiltration to promote glioma deterioration.

Applications

Unspecified application

Species

Unspecified reactive species

Shupeng Li,Ziwei Li,Xinyu Wang,Junzhe Zhong,Daohan Yu,Hao Chen,Wenbin Ma,Lingling Liu,Minghuang Ye,Ruofei Shen,Chuanlu Jiang,Xiangqi Meng,Jinquan Cai
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com