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Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (AB300138), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFIHC-PFlow Cyt
Human
Not recommended
Not recommended
Tested
Not recommended
Mouse
Not recommended
Not recommended
Not recommended
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Not recommended
Transfected cell line - Human
Not recommended
Not recommended
Tested
Not recommended

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Transfected cell line - Human
Dilution info
1/4000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human, Mouse, Rat, Transfected cell line - Human
Dilution info
-
Notes

-

Target data

Function

Cytokine secreted primarily by mast cells, T-cells, eosinophils, and basophils that plays a role in regulating antibody production, hematopoiesis and inflammation, and the development of effector T-cell responses (PubMed:1993171, PubMed:3016727). Induces the expression of class II MHC molecules on resting B-cells. Enhances both secretion and cell surface expression of IgE and IgG1 (PubMed:1993171). Regulates also the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes (PubMed:2521231). Positively regulates IL31RA expression in macrophages. Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4. In addition, plays a critical role in higher functions of the normal brain, such as memory and learning (By similarity). Upon binding to IL4, IL4R receptor dimerizes either with the common IL2R gamma chain/IL2RG to produce the type 1 signaling complex, located mainly on hematopoietic cells, or with the IL13RA1 to produce the type 2 complex, which is expressed also on nonhematopoietic cells (PubMed:10219247, PubMed:11526337, PubMed:18243101). Engagement of both types of receptors initiates JAK3 and to a lower extend JAK1 phosphorylation leading to activation of the signal transducer and activator of transcription 6/STAT6 (PubMed:7721895).

Alternative names

Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4

Recommended products

Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR25269-115
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag. labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positivie staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temerature.
    The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). 

    Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag, labeling IL-4 with ab300138 at 1/4000 dilution (0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).

    Positive staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temperature. 

    The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). 

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IL4 antibody [EPR25269-115] (ab300138)

    Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
    The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. 

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

    Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). 

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Product protocols

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