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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
WB | ICC/IF | IHC-P | Flow Cyt | |
---|---|---|---|---|
Human | Not recommended | Not recommended | Tested | Not recommended |
Mouse | Not recommended | Not recommended | Not recommended | Not recommended |
Rat | Not recommended | Not recommended | Not recommended | Not recommended |
Transfected cell line - Human | Not recommended | Not recommended | Tested | Not recommended |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Transfected cell line - Human | Dilution info 1/4000 | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Participates in at least several B-cell activation processes as well as of other cell types (PubMed:3016727). It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages (By similarity). Stimulates autophagy in dendritic cells by interfering with mTORC1 signaling and through the induction of RUFY4 (By similarity).
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
Rabbit Monoclonal IL-4 antibody. Suitable for IHC-P and reacts with Human, Transfected cell line - Human samples. Cited in 1 publication.
Interleukin-4, IL-4, B-cell stimulatory factor 1, Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra, BSF-1, IL4
IgG
Rabbit
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Liquid
Monoclonal
EPR25269-115
Affinity purification Protein A
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag. labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positivie staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temerature.
The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: Secondary antibody is a rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on scattered immune cells of human tonsil. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemical analysis of paraffin-embedded human cardiac muscle labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control: no staining in human cardiac muscle. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Immunohistochemical analysis of paraffin-embedded: Panel A : HEK-293T cells transfected with a Interleukin-4 expression vector containing a his tag. Panel B: HEK-293T cells transfected with empty vector containing a his tag, labeling IL-4 with ab300138 at 1/4000 dilution (0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Positive staining on (A) HEK-293T transfected with a human IL-4 expression vector; no staining on (B) HEK-293T transfected with empty vector. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
Immunohistochemical analysis of paraffin-embedded human NKT cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human NKT cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
Immunohistochemical analysis of paraffin-embedded Human anaplastic large cell lymphoma labeling IL-4 with ab300138 at 1/4000 dilution(0.14ug/ml), followed by ready to use rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on scattered immune cells of human anaplastic large cell lymphoma. The section was incubated with ab300138 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.
Secondary antibody only control: used PBS instead of primary antibody, followed by secondary antibody (ready to use rabbit specific IHC polymer detection kit HRP/DAB) (ab209101).
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