Goat Polyclonal ILF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FOXK2 aa 600 to C-terminus.
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Publications
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- Protein & cell 12:557-5772020Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYu-Kun Xia,Yi-Rong Zeng,Meng-Li Zhang,Peng Liu,Fang Liu,Hao Zhang,Chen-Xi He,Yi-Ping Sun,Jin-Ye Zhang,Cheng Zhang,Lei Song,Chen Ding,Yu-Jie Tang,Zhen Yang,Chen Yang,Pu Wang,Kun-Liang Guan,Yue Xiong,Dan YePubMed 32683582
- Oncogenesis 7:292018SUMOylation modulates FOXK2-mediated paclitaxel sensitivity in breast cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesGabriela Nestal de Moraes et. al.PubMed 29540677
- International journal of cancer 142:2543-25572018FOXK2 suppresses the malignant phenotype and induces apoptosis through inhibition of EGFR in clear-cell renal cell carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFan Zhang et. al.PubMed 29368368
- Scientific reports 5:87962015FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYing Liu et. al.PubMed 25740706
- Nucleic acids research 42:6232-422014The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex.
- Molecular and cellular biology 32:385-982011The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation.Applications:WB, ChIPReactive species:HumanZongling Ji,Ian J Donaldson,Jingru Liu,Andrew Hayes,Leo A H Zeef,Andrew D SharrocksPubMed 22083952
- The Journal of biological chemistry 285:35728-392010Cell cycle-dependent regulation of the forkhead transcription factor FOXK2 by CDK·cyclin complexes.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Human FOXK2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q01167
Reactivity data
Select an application| WB | ICC/IF | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.01000-0.03000 µg/mL | Notes A 1 hour primary incubation is recommended for this product. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
Associated Products
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Target data
Function
Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed:22083952, PubMed:25451922). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle: in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (PubMed:22083952). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (PubMed:20097901). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (PubMed:1339390). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (PubMed:25805136). Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements (PubMed:1909027). Accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex; recruits the PR-DUB complex to specific FOXK2-bound genes (PubMed:24634419, PubMed:30664650).
Alternative names
ILF, ILF1, FOXK2, Forkhead box protein K2, G/T-mismatch specific binding protein, Interleukin enhancer-binding factor 1, nGTBP
Recommended products
Goat Polyclonal ILF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human FOXK2 aa 600 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Goat
- Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Human FOXK2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q01167
- Purification technique
- Affinity purification Immunogen
- Concentration
- Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
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Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The protein Interleukin Enhancer Binding Factor 1 (ILF1) also known as Nuclear Factor of Activated T-cells 90 (NF90) plays an essential role in the regulation of gene expression. ILF1 has a mass of approximately 90 kDa (kilodaltons) and is ubiquitously expressed in various tissues including the spleen and the thymus. The protein consists of two distinct ribonucleoprotein (RNP) motifs that allow it to bind RNA sequences effectively making it an important factor in controlling mRNA stability and transport.
- Biological function summary
ILF1 influences several cellular processes related to RNA dynamics by engaging in RNA splicing export and translation regulation. It doesn't function alone but rather forms complexes with other proteins such as the nuclear factor 45 (NF45). Through these interactions ILF1 contributes actively to cellular stress responses influencing protein synthesis under stress conditions and aiding in regulating cytokine production.
- Pathways
ILF1 intricately integrates into cellular pathways like the NF-κB signaling pathway and the T-cell receptor signaling pathway. In these contexts it interacts with proteins such as IκB kinase (IKK) and T-cell receptor-associated proteins playing a part in transcriptional regulation and immune response modulation. ILF1's involvement in these pathways highlights its importance in cellular adaptation and survival in response to external stressors and pathogens.
- Associated diseases and disorders
ILF1 is associated with conditions such as viral infections and cancer. In viral infections the protein interacts with viral RNAs impacting viral replication and host cell responses. In cancer ILF1's aberrant regulation could promote tumor progression by affecting cell proliferation and apoptosis pathways as seen in its relationship with proteins like p53 and Bcl-2. As a potential biomarker and therapeutic target understanding ILF1's pathological connections could improve diagnostic and treatment strategies for these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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5 product images
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.
Flow Cytometry (Intracellular) - Anti-ILF1 antibody (ab5298)
Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labeling ILF1 with ab5298. Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (1 μg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Western blot - Anti-ILF1 antibody (ab5298)
All lanes: Western blot - Anti-ILF1 antibody (ab5298) at 0.03 µg/mL
Lane 1: HEK-293 (human epithelial cell line from embryonic kidney) nuclear cell lysate at 35 µg
Lane 2: HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate at 35 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) nuclear cell lysate at 35 µg
Predicted band size: 69 kDa
Observed band size: 80 kDa
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (ab5298)
Immunofluorescent analysis of paraformaldehyde fixed U2OS cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).
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