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AB5298

Anti-ILF1 antibody

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(10 Publications)

Goat Polyclonal ILF1 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 10 publications. Immunogen corresponding to Synthetic Peptide within Human FOXK2 aa 600 to C-terminus.

View Alternative Names

ILF, ILF1, FOXK2, Forkhead box protein K2, G/T-mismatch specific binding protein, Interleukin enhancer-binding factor 1, nGTBP

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)

Immunofluorescent analysis of paraformaldehyde fixed HeLa cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).

Flow Cytometry (Intracellular) - Anti-ILF1 antibody (AB5298)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ILF1 antibody (AB5298)

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) labeling ILF1 with ab5298. Cells permeabilized with 0.5% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (1 μg/mL). IgG control : Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)

Immunofluorescent analysis of paraformaldehyde fixed U2OS cells, labeling ILF-1 with ab5298. Cells permeabilized with 0.15% Triton. Primary incubation 1hr (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL), showing nuclear and cytoplasmic/vesicle staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor 488 secondary antibody (2 μg/mL).

Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)
  • ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-ILF1 antibody (AB5298)

Immunofluorescence analysis of U2OS cells, staining ILF1 with ab5298. Cells were fixed with 4% paraformaldehyde and incubated with primary antibody at 1/100 dilution. A FITC-conjugated rabbit anti-goat IgG was used as the secondary antibody.

Image from Marais A et al., J Biol Chem. 2010 Nov 12;285(46):35728-39. Epub 2010 Sep 1. Fig 2.; doi: 10.1074/jbc.M110.154005; November 12, 2010, The Journal of Biological Chemistry, 285, 35728-35739.

Western blot - Anti-ILF1 antibody (AB5298)
  • WB

Supplier Data

Western blot - Anti-ILF1 antibody (AB5298)

All lanes:

Western blot - Anti-ILF1 antibody (ab5298) at 0.03 µg/mL

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) nuclear cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) nuclear cell lysate at 35 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) nuclear cell lysate at 35 µg

Predicted band size: 69 kDa

Observed band size: 80 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt (Intra), ICC/IF

applications

Immunogen

Synthetic Peptide within Human FOXK2 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q01167

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.01-0.03 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "10 µg/mL", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 0.5% BSA, 0.5% Tris buffered saline
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein Interleukin Enhancer Binding Factor 1 (ILF1) also known as Nuclear Factor of Activated T-cells 90 (NF90) plays an essential role in the regulation of gene expression. ILF1 has a mass of approximately 90 kDa (kilodaltons) and is ubiquitously expressed in various tissues including the spleen and the thymus. The protein consists of two distinct ribonucleoprotein (RNP) motifs that allow it to bind RNA sequences effectively making it an important factor in controlling mRNA stability and transport.
Biological function summary

ILF1 influences several cellular processes related to RNA dynamics by engaging in RNA splicing export and translation regulation. It doesn't function alone but rather forms complexes with other proteins such as the nuclear factor 45 (NF45). Through these interactions ILF1 contributes actively to cellular stress responses influencing protein synthesis under stress conditions and aiding in regulating cytokine production.

Pathways

ILF1 intricately integrates into cellular pathways like the NF-κB signaling pathway and the T-cell receptor signaling pathway. In these contexts it interacts with proteins such as IκB kinase (IKK) and T-cell receptor-associated proteins playing a part in transcriptional regulation and immune response modulation. ILF1's involvement in these pathways highlights its importance in cellular adaptation and survival in response to external stressors and pathogens.

ILF1 is associated with conditions such as viral infections and cancer. In viral infections the protein interacts with viral RNAs impacting viral replication and host cell responses. In cancer ILF1's aberrant regulation could promote tumor progression by affecting cell proliferation and apoptosis pathways as seen in its relationship with proteins like p53 and Bcl-2. As a potential biomarker and therapeutic target understanding ILF1's pathological connections could improve diagnostic and treatment strategies for these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional regulator involved in different processes such as glucose metabolism, aerobic glycolysis and autophagy (By similarity). Recognizes and binds the forkhead DNA sequence motif (5'-GTAAACA-3') and can both act as a transcription activator or repressor, depending on the context (PubMed : 22083952, PubMed : 25451922). Together with FOXK1, acts as a key regulator of metabolic reprogramming towards aerobic glycolysis, a process in which glucose is converted to lactate in the presence of oxygen (By similarity). Acts by promoting expression of enzymes for glycolysis (such as hexokinase-2 (HK2), phosphofructokinase, pyruvate kinase (PKLR) and lactate dehydrogenase), while suppressing further oxidation of pyruvate in the mitochondria by up-regulating pyruvate dehydrogenase kinases PDK1 and PDK4 (By similarity). Probably plays a role in gluconeogenesis during overnight fasting, when lactate from white adipose tissue and muscle is the main substrate (By similarity). Together with FOXK1, acts as a negative regulator of autophagy in skeletal muscle : in response to starvation, enters the nucleus, binds the promoters of autophagy genes and represses their expression, preventing proteolysis of skeletal muscle proteins (By similarity). In addition to the 5'-GTAAACA-3' DNA motif, also binds the 5'-TGANTCA-3' palindromic DNA motif, and co-associates with JUN/AP-1 to activate transcription (PubMed : 22083952). Also able to bind to a minimal DNA heteroduplex containing a G/T-mismatch with 5'-TRT[G/T]NB-3' sequence (PubMed : 20097901). Binds to NFAT-like motifs (purine-rich) in the IL2 promoter (PubMed : 1339390). Positively regulates WNT/beta-catenin signaling by translocating DVL proteins into the nucleus (PubMed : 25805136). Also binds to HIV-1 long terminal repeat. May be involved in both positive and negative regulation of important viral and cellular promoter elements (PubMed : 1909027). Accessory component of the polycomb repressive deubiquitinase (PR-DUB) complex; recruits the PR-DUB complex to specific FOXK2-bound genes (PubMed : 24634419, PubMed : 30664650).
See full target information FOXK2
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cancer letters 597:217074 PubMed38901667

2024

FOXK2 amplification promotes breast cancer development and chemoresistance.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Yu,Wen-Ming Cao,Feng Cheng,Zhongcheng Shi,Lili Han,Jinling Yi,Edaise M da Silva,Higinio Dopeso,Hui Chen,Jianhua Yang,Xiaosong Wang,Chunchao Zhang,Hong Zhang

Oncogene 43:1985-1999 PubMed38734828

2024

Phosphorylation of FOXK2 at Thr13 and Ser30 by PDK2 sustains glycolysis through a positive feedback manner in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Cancan Zhang,Yinyin Xu,Xinyue Zhu,Xueli Zhang,Fengmian Wang,Lipeng Hu,Huan Lu,Chunlin Tao,Kai Xu,Zhigang Zhang,Dongxue Li,Tingyan Shi,Rong Zhang

International journal of biological sciences 17:2294-2307 PubMed34239356

2021

CircHIPK3 regulates pulmonary fibrosis by facilitating glycolysis in miR-30a-3p/FOXK2-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Xu,Demin Cheng,Guanru Li,Yi Liu,Ping Li,Wenqing Sun,Dongyu Ma,Chunhui Ni

Protein & cell 12:557-577 PubMed32683582

2020

Tumor-derived neomorphic mutations in ASXL1 impairs the BAP1-ASXL1-FOXK1/K2 transcription network.

Applications

Unspecified application

Species

Unspecified reactive species

Yu-Kun Xia,Yi-Rong Zeng,Meng-Li Zhang,Peng Liu,Fang Liu,Hao Zhang,Chen-Xi He,Yi-Ping Sun,Jin-Ye Zhang,Cheng Zhang,Lei Song,Chen Ding,Yu-Jie Tang,Zhen Yang,Chen Yang,Pu Wang,Kun-Liang Guan,Yue Xiong,Dan Ye

Oncogenesis 7:29 PubMed29540677

2018

SUMOylation modulates FOXK2-mediated paclitaxel sensitivity in breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriela Nestal de Moraes,Zongling Ji,Lavender Y-N Fan,Shang Yao,Stefania Zona,Andrew D Sharrocks,Eric W-F Lam

International journal of cancer 142:2543-2557 PubMed29368368

2018

FOXK2 suppresses the malignant phenotype and induces apoptosis through inhibition of EGFR in clear-cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Zhang,Xin Ma,Hongzhao Li,Yu Zhang,Xintao Li,Luyao Chen,Gang Guo,Yu Gao,Liangyou Gu,Yongpeng Xie,Junyao Duan,Xu Zhang

Scientific reports 5:8796 PubMed25740706

2015

FOXK2 transcription factor suppresses ERα-positive breast cancer cell growth through down-regulating the stability of ERα via mechanism involving BRCA1/BARD1.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Liu,Xiang Ao,Zhaojun Jia,Xiao-Yan Bai,Zhaowei Xu,Gaolei Hu,Xiao Jiang,Min Chen,Huijian Wu

Nucleic acids research 42:6232-42 PubMed24748658

2014

The forkhead transcription factor FOXK2 acts as a chromatin targeting factor for the BAP1-containing histone deubiquitinase complex.

Applications

IP

Species

Unspecified reactive species

Zongling Ji,Hisham Mohammed,Aaron Webber,Jenna Ridsdale,Namshik Han,Jason S Carroll,Andrew D Sharrocks

Molecular and cellular biology 32:385-98 PubMed22083952

2011

The forkhead transcription factor FOXK2 promotes AP-1-mediated transcriptional regulation.

Applications

WB, ChIP

Species

Human, Human

Zongling Ji,Ian J Donaldson,Jingru Liu,Andrew Hayes,Leo A H Zeef,Andrew D Sharrocks

The Journal of biological chemistry 285:35728-39 PubMed20810654

2010

Cell cycle-dependent regulation of the forkhead transcription factor FOXK2 by CDK·cyclin complexes.

Applications

WB, ICC/IF

Species

Human, Human

Anett Marais,Zongling Ji,Emma S Child,Eberhard Krause,David J Mann,Andrew D Sharrocks
View all publications
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