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AB131004

Anti-ILF3 antibody

4

(1 Review)

|

(7 Publications)

Rabbit Polyclonal ILF3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ILF3 aa 650-750.

View Alternative Names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

2 Images
Immunoprecipitation - Anti-ILF3 antibody (AB131004)
  • IP

Unknown

Immunoprecipitation - Anti-ILF3 antibody (AB131004)

Detection of ILF3 by Western Blot of Immunprecipitate.

ab131004 at 1μg/ml staining ILF3 in HeLa whole cell lysate immunoprecipitated using ab131004 at 6μg/mg lysate (1 mg/IP; 20% of IP loaded/lane).

Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ILF3 antibody (ab131004)

Predicted band size: 95 kDa

false

Western blot - Anti-ILF3 antibody (AB131004)
  • WB

Unknown

Western blot - Anti-ILF3 antibody (AB131004)

All lanes:

Western blot - Anti-ILF3 antibody (ab131004) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 95 kDa

true

Exposure time: 10s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ILF3 aa 650-750. The exact immunogen used to generate this antibody is proprietary information.

Q12906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Opossum": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab131004 is affinity purified using an epitope specific to ILF3 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.
Biological function summary

ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.

Pathways

ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.

ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
See full target information ILF3
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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:1986 PubMed40011431

2025

8q24 derived ZNF252P promotes tumorigenesis by driving phase separation to activate c-Myc mediated feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Tianyu Qu,Chang Zhang,Xiyi Lu,Jiali Dai,Xuezhi He,Wei Li,Liang Han,Dandan Yin,Erbao Zhang

RNA biology 18:287-302 PubMed34130584

2021

Long non-coding RNA RP11-283G6.5 confines breast cancer development through modulating miR-188-3p/TMED3/Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Pei,Shengquan Zhang,Xiaowei Yang,Chunguang Han,Yubo Pan,Jun Li,Zhaorui Wang,Chenyu Sun,Jing Zhang

Frontiers in oncology 10:586085 PubMed34094894

2021

FUT8-AS1 Inhibits the Malignancy of Melanoma Through Promoting miR-145-5p Biogenesis and Suppressing NRAS/MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang-Jun Chen,Sha Liu,Dong-Mei Han,De-Zhi Han,Wei-Jing Sun,Xiao-Chun Zhao,Jun-Qing Liang,Li Yu

International journal of molecular medicine 47: PubMed33693959

2021

Upregulated long non‑coding RNA LincIN promotes tumor progression via the regulation of nuclear factor 90/microRNA‑7/HOXB13 in esophageal squamous cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zhibo Tan,Peitao Zhou,Zhenru Zhu,Ying Wang,Zeqin Guo,Mengying Shen,Yazhi Xiao,Weixi Shen,Dehua Wu

RNA biology 18:547-562 PubMed32951513

2020

Genomic amplification of long noncoding RNA HOTAIRM1 drives anaplastic thyroid cancer progression via repressing miR-144 biogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Ling Zhang,Jin Zhang,Shujing Li,Yanyan Zhang,Yun Liu,Jian Dong,Wenjun Zhao,Bo Yu,Huifang Wang,Jing Liu

Journal of Cancer 10:5139-5152 PubMed31602267

2019

An NF90/long noncoding RNA-LET/miR-548k feedback amplification loop controls esophageal squamous cell carcinoma progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianqing Lin,Zhiyao Chen,Shanhu Wu,Wenbo Huang,Feng Chen,Zhijun Huang

Breast cancer research : BCR 19:62 PubMed28558830

2017

LincIN, a novel NF90-binding long non-coding RNA, is overexpressed in advanced breast tumors and involved in metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengyu Jiang,Carolyn M Slater,Yan Zhou,Karthik Devarajan,Karen J Ruth,Yueran Li,Kathy Q Cai,Mary Daly,Xiaowei Chen
View all publications
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Product promise

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