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AB327172

Anti-ILF3 Antibody

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Rabbit Polyclonal ILF3 antibody. Suitable for WB, IHC-P, ICC/IF, ChIP and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human ILF3.

View Alternative Names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)

Immunohistochemical staining of human cerebral cortex using ab327172 at 1 : 2500–1 : 5000 dilution, showing strong nuclear positivity in neurons.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)

Immunohistochemical staining of human pancreas using ab327172 at 1 : 2500–1 : 5000 dilution, showing strong nuclear positivity in exocrine glandular cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)

Immunohistochemical staining of human lymph node using ab327172 at 1 : 2500–1 : 5000 dilution, showing strong nuclear positivity in germinal center cells.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 Antibody (AB327172)

Immunohistochemical staining of human lymph node using ab327172 at 1 : 2500–1 : 5000 dilution, showing strong nuclear positivity in cells in seminiferous ducts.

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 Antibody (AB327172)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 Antibody (AB327172)

Immunofluorescent staining of human cell line U-251 MG using ab327172 at 0.25–2 µg/ml, showing localization to nucleoplasm, nucleoli & mitochondria.

ChIP - Anti-ILF3 Antibody (AB327172)
  • ChIP

Supplier Data

ChIP - Anti-ILF3 Antibody (AB327172)

ChIP-Exo-Seq composite graph for ab327172 tested in K562 cells at 1–10 µg per reaction. Strand-specific reads (blue : forward, red : reverse) and IgG controls (black : forward, grey : reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ChIP, IHC-P, ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human ILF3.

Q12906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2500 - 1/5000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p></p>", "ChIP-species-checked": "testedAndGuaranteed", "ChIP-species-dilution-info": "1-10 µg", "ChIP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
See full target information ILF3
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Product promise

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