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AB89100

Anti-ILF3 antibody

4

(1 Review)

|

(3 Publications)

Mouse Polyclonal ILF3 antibody. Carrier free. Suitable for ICC, WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ILF3.

View Alternative Names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

5 Images
Immunocytochemistry - Anti-ILF3 antibody (AB89100)
  • ICC

Unknown

Immunocytochemistry - Anti-ILF3 antibody (AB89100)

Immunocytochemistry analysis of HeLa cells labeling ILF3 with ab89100 at 10 μg/mL.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody (AB89100)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody (AB89100)

ab89100, at 3μg/ml, staining human ILF3in small intestine, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

Western blot - Anti-ILF3 antibody (AB89100)
  • WB

Unknown

Western blot - Anti-ILF3 antibody (AB89100)

All lanes:

Western blot - Anti-ILF3 antibody (ab89100) at 1 µg/mL

All lanes:

HeLa cell lysate at 50 µg

Predicted band size: 95 kDa

Observed band size: 105 kDa,95 kDa

false

Western blot - Anti-ILF3 antibody (AB89100)
  • WB

Unknown

Western blot - Anti-ILF3 antibody (AB89100)

All lanes:

Western blot - Anti-ILF3 antibody (ab89100) at 1 µg/mL

Lane 1:

293T transfected cell lysate at 25 µg

Lane 2:

Non-transfected lysate at 25 µg

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Western blot - Anti-ILF3 antibody (AB89100)
  • WB

Unknown

Western blot - Anti-ILF3 antibody (AB89100)

All lanes:

Western blot - Anti-ILF3 antibody (ab89100) at 1 µg/mL

All lanes:

Human pancreas lysate at 50 µg

Predicted band size: 95 kDa

Observed band size: 80 kDa,95 kDa

false

Key facts

Host species

Mouse

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB, ICC

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ILF3.

Q12906

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "3 µg/mL", "IHCP-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.
Biological function summary

ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.

Pathways

ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.

ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
See full target information ILF3
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The American Journal of dermatopathology 44:575-580 PubMed35503885

2022

PRAME Expression Correlates With Genomic Aberration and Malignant Diagnosis of Spitzoid Melanocytic Neoplasms.

Applications

Unspecified application

Species

Unspecified reactive species

Pedram Gerami,Sarah Benton,Jeffrey Zhao,Bin Zhang,Nathaniel Lampley,Andrew Roth,Anastasiya Boutko,Shantel Olivares,Klaus J Busam

Journal of experimental & clinical cancer research 38:439 PubMed31747963

2019

Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Jian-Xing Wang,Zi-Yuan Nie,Yue Wen,Xin-Ju Jia,Li-Na Zhang,Hui-Jun Duan,Yong-Hong Shi

Cell death and differentiation 26:1700-1715 PubMed30464226

2018

Long noncoding RNA lncAIS downregulation in mesenchymal stem cells is implicated in the pathogenesis of adolescent idiopathic scoliosis.

Applications

Unspecified application

Species

Unspecified reactive species

Qianyu Zhuang,Buqing Ye,Shangyi Hui,Ying Du,Robert Chunhua Zhao,Jing Li,Zhihong Wu,Na Li,Yanbin Zhang,Hongling Li,Shengru Wang,Yang Yang,Shugang Li,Hong Zhao,Zusen Fan,Guixing Qiu,Jianguo Zhang
View all publications
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