Anti-ILF3 antibody [EPR3626] (ab92355) is a rabbit monoclonal antibody detecting ILF3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2010
Images
![Western blot - Anti-ILF3 antibody [EPR3626] (AB92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--western-blot-img88179.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ILF3 antibody [EPR3626] (AB92355)")
![Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (AB92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--flow-cytometry-intracellular-img27540.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (AB92355)")
![Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (AB92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--immunocytochemistry-immunofluorescence-img68103.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (AB92355)")
![Western blot - Anti-ILF3 antibody [EPR3626] (AB92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--western-blot-img126285.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ILF3 antibody [EPR3626] (AB92355)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (AB92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117719.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (AB92355)")
Publications
Filter by
- Cancers 15:2023SOX2-OT Binds with ILF3 to Promote Head and Neck Cancer Progression by Modulating Crosstalk between STAT3 and TGF-β Signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRu Wang,Yifan Yang,Lingwa Wang,Qian Shi,Hongzhi Ma,Shizhi He,Ling Feng,Jugao FangPubMed 38136312
- Biology direct 18:332023PSMD3-ILF3 signaling cascade drives lung cancer cell proliferation and migration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJin Zhang,Qianli Ma,Qiduo Yu,Fei Xiao,Zhenrong Zhang,Hongxiang Feng,Chaoyang LiangPubMed 37337223
- iScience 26:1062292023ILF3 prion-like domain regulates gene expression and fear memory under chronic stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAkira Yamashita,Yuichi Shichino,Kazuki Fujii,Yumie Koshidaka,Mayumi Adachi,Eri Sasagawa,Mari Mito,Shinichi Nakagawa,Shintaro Iwasaki,Keizo Takao,Nobuyuki ShiinaPubMed 36876121
- Genome biology 23:1692022Survey of the binding preferences of RNA-binding proteins to RNA editing events.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaolin Hu,Qin Zou,Li Yao,Xuerui YangPubMed 35927743
- Scientific reports 12:88372022NF90-NF45 is essential for β cell compensation under obesity-inducing metabolic stress through suppression of p53 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSylvia Lai,Takuma Higuchi,Masayuki Tsuda,Yasunori Sugiyama,Keiko Morisawa,Taketoshi Taniguchi,Shuji SakamotoPubMed 35614067
- Cell cycle (Georgetown, Tex.) 21:1034-10472022LncRNA LINC00649 promotes the growth and metastasis of triple-negative breast cancer by maintaining the stability of HIF-1α through the NF90/NF45 complex.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianhua Zhang,Chuang Du,Linfeng Zhang,Yan Wang,Yingying Zhang,Jingruo LiPubMed 35188449
- Journal of immunology (Baltimore, Md. : 1950) 206:2949-29652021ILF3 Is a Negative Transcriptional Regulator of Innate Immune Responses and Myeloid Dendritic Cell Maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRodolfo Nazitto,Lynn M Amon,Fred D Mast,John D Aitchison,Alan Aderem,Jarrod S Johnson,Alan H DiercksPubMed 34031149
- Epigenetics & chromatin 14:32021Genome-wide occupancy reveals the localization of H1T2 (H1fnt) to repeat regions and a subset of transcriptionally active chromatin domains in rat spermatids.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVasantha Shalini et. al.PubMed 33407810
- Cell biology international 44:1671-16802020LINC00667 promotes the proliferation, migration, and pathological angiogenesis in non-small cell lung cancer through stabilizing VEGFA by EIF4A3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuizhen Yang,Wuchen Yang,Wanqing Dai,Yun Ma,Guojun ZhangPubMed 32281700
- Oncology letters 19:2141-21522020High expression of interleukin-enhancer binding factor 3 predicts poor prognosis in patients with lung adenocarcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhangyan Xu et. al.PubMed 32194712
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | Flow Cyt (Intra) | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Tested | Tested | Tested |
| Mouse | Expected | Tested | Expected | Expected |
| Rat | Expected | Tested | Expected | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/10000 | Notes For unpurified use at 1/100 - 1/250. The use of an HRP/AP polymerized antibody is recommended as these have been shown to provide enhanced staining. Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/10000 - 1/100000 | Notes - |
Species Rat | Dilution info 1/10000 - 1/100000 | Notes - |
Species Human | Dilution info 1/10000 - 1/100000 | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 - 1/60 | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/250 - 1/500 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed:28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed:14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed:21123651, PubMed:34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed:21123651, PubMed:28625552). (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
Alternative names
DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80
Recommended products
Anti-ILF3 antibody [EPR3626] (ab92355) is a rabbit monoclonal antibody detecting ILF3 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IHC-P, ICC/IF. Suitable for Human, Mouse, Rat.
- Biophysical QC for unrivalled batch-batch consistency
- Over 20 publications
- Trusted since 2010
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3626
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C
Notes
Anti-ILF3 antibody [EPR3626] (ab92355) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.
What is the molecular weight of ILF3?
Anti-ILF3 [EPR3626] (ab92355) specifically detects a band for ILF3 (UniProt: Q12906) at a molecular weight of 95kDa.
Trusted by the scientific community
Anti-ILF3 [EPR3626] (ab92355) was first used in a scientific publication in 2010 and has been cited over 20 times in peer-reviewed journals.
Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.
Other related products
We have a range of other formats of antibody clone [EPR3626] also available for your convenience:
ab92355, HRP - HRP Anti-ILF3 antibody [EPR3626] ab206250, Carrier free - Anti-ILF3 antibody [EPR3626] - BSA and Azide free ab232546
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.
- Biological function summary
ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.
- Pathways
ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.
- Associated diseases and disorders
ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Western blot - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--western-blot-img88179.jpg "Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)")
Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
Blocking and diluting buffer: 5% NFDM/TBST.
The two bands are reported by PMID: 22842455 and 27872311.All lanes: Western blot - Anti-ILF3 antibody [EPR3626] (ab92355) at 1/100000 dilution
Lane 1: Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg
Lane 2: SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates at 20 µg
Lane 3: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates at 20 µg
Lane 4: Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates at 20 µg
Lane 5: C6 (Rat glial tumor glial cell) whole cell lysates at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 95 kDa
![Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--flow-cytometry-intracellular-img27540.jpg "Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355)")
Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355)
Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with purified ab92355 at 1/60 dilution (10μg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
![Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--immunocytochemistry-immunofluorescence-img68103.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (ab92355)")
Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3626] (ab92355)
Immunofluorescence staining of HeLa cells with purified ab92355 at a working dilution of 1/500, counter-stained with DAPI. The secondary antibody was an Alexa Fluor® 488 conjugated goat anti-rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077), used at a dilution of 1/1000. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative control is shown in bottom right hand panel - for the negative control, PBS was used instead of the primary antibody.
![Western blot - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--western-blot-img126285.jpg "Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)")
Western blot - Anti-ILF3 antibody [EPR3626] (ab92355)
All lanes: Western blot - Anti-ILF3 antibody [EPR3626] (ab92355) at 1/100000 dilution
Lane 1: Raji cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: K562 cell lysate at 10 µg
SecondaryAll lanes: HRP labelled goat anti-rabbit antibody at 1/2000 dilution
Predicted band size: 95 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117719.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (ab92355)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3626] (ab92355)
ab92355 (unpurified) at 1/100 dilution staining ILF3 in paraffin-embedded Human kidney tissue, by immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--flow-cytometry-intracellular-img415568.jpg "Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355)")
Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355)
Overlay histogram showing HeLa cells stained with unpurified ab92355 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92355, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
![Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355), expandable thumbnail](https://content.abcam.com/products/images/ilf3-antibody-epr3626-ab92355--flow-cytometry-intracellular-img415568.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (ab92355)")
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com