JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB133354

Anti-ILF3 antibody [EPR3627]

Be the first to review this product! Submit a review

|

(5 Publications)

Rabbit Recombinant Monoclonal ILF3 antibody. Suitable for IHC-P, ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 5 publications.

View Alternative Names

DRBF, MPHOSPH4, NF90, ILF3, Interleukin enhancer-binding factor 3, Double-stranded RNA-binding protein 76, M-phase phosphoprotein 4, Nuclear factor associated with dsRNA, Nuclear factor of activated T-cells 90 kDa, Translational control protein 80, DRBP76, MPP4, NFAR, NF-AT-90, TCP80

5 Images
Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3627] (AB133354)
  • Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3627] (AB133354)

Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3627] (AB133354)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3627] (AB133354)

Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (2.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3627] (AB133354)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3627] (AB133354)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling ILF3 with Purified ab133354 at 1/6000 dilution (0.09 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-ILF3 antibody [EPR3627] (AB133354)
  • WB

Unknown

Western blot - Anti-ILF3 antibody [EPR3627] (AB133354)

ab133354 recognizes only NF110 isoform of ILF3.

All lanes:

Western blot - Anti-ILF3 antibody [EPR3627] (ab133354) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 110 kDa

false

Western blot - Anti-ILF3 antibody [EPR3627] (AB133354)
  • WB

Unknown

Western blot - Anti-ILF3 antibody [EPR3627] (AB133354)

ab133354 recognizes only NF110 isoform of ILF3.

All lanes:

Western blot - Anti-ILF3 antibody [EPR3627] (ab133354) at 1/20000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa,63 kDa,95 kDa

Observed band size: 110 kDa,30 kDa,63 kDa,65 kDa

false

  • Carrier free

    Anti-ILF3 antibody [EPR3627] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3627

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra), IHC-P, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB89100

Anti-ILF3 antibody

Immunocytochemistry - Anti-ILF3 antibody (AB89100)

  • 4
  • 1
Primary Antibodies

AB225626

Anti-ILF3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody (AB225626)

  • 0
  • 0
Primary Antibodies

AB131004

Anti-ILF3 antibody

Immunoprecipitation - Anti-ILF3 antibody (AB131004)

  • 4
  • 1
Primary Antibodies

AB206250

HRP Anti-ILF3 antibody [EPR3626]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HRP Anti-ILF3 antibody [EPR3626] (AB206250)

  • 0
  • 0
Primary Antibodies

AB92355

Anti-ILF3 antibody [EPR3626]

20ul selling size|RabMAb|Recombinant|Trial Size

Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] (AB92355)

  • 4
  • 1
Primary Antibodies

AB232546

Anti-ILF3 antibody [EPR3626] - BSA and Azide free

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3626] - BSA and Azide free (AB232546)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB125011

Anti-TSG101 antibody [EPR7130(B)]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Anti-TSG101 antibody [EPR7130(B)] (AB125011)

  • 4
  • 10
Primary Antibodies

AB134045

Anti-CD63 antibody [EPR5702] - Late Endosome Marker

20ul selling size|RabMAb|Recombinant|KO Validated|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD63 antibody [EPR5702] - Late Endosome Marker (AB134045)

  • 3
  • 6
Primary Antibodies

AB7817

Anti-alpha smooth muscle Actin antibody [1A4]

KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (AB7817)

  • 5
  • 105
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/6000", "IHCP-species-notes": "<p><strong>For unpurified use at 1/100 - 1/250.</strong></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/250", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/5000", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "<p></p>" } } }
style
left-column

Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ILF3 protein also known as Interleukin enhancer-binding factor 3 plays an important role in cellular mechanisms. It has a molecular mass of approximately 90 kDa and is expressed broadly in various tissues such as the liver kidney and spleen. ILF3 is part of the double-stranded RNA-binding proteins and it engages in regulating RNA metabolism processes. Scientists also refer to ILF3 as NF90 and it is known for its function in modulating gene expression by interacting with RNA.
Biological function summary

ILF3 interacts with RNA and proteins within the cell to control the stability and translation of mRNA. It acts as a component of various ribonucleoprotein complexes indicating its role in post-transcriptional regulation. ILF3 can bind to double-stranded RNA and single-stranded RNA motifs affecting the proliferation and differentiation of cells in which it is expressed. It also contributes significantly in cellular stress responses impacting how cells respond to environmental changes.

Pathways

ILF3 is involved in important cellular pathways such as RNA processing and antiviral defense mechanisms. In the RNA processing pathway ILF3 associates with proteins such as Drosha impacting microRNA biogenesis. Additionally in antiviral responses it identifies and binds viral RNAs initiating defensive cellular responses. These pathways position ILF3 as integral to maintaining cellular homeostasis during stress and infection.

ILF3 has connections to cancer and viral infections. Aberrations in ILF3 expression or function are observed in certain cancers where it influences tumor progression by interacting with oncogenic pathways. In viral infections like those caused by hepatitis C virus ILF3 contributes to host defense by modulating immune responses. It interacts with proteins like RIG-I in the context of viral infections facilitating antiviral signaling pathways to control disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

RNA-binding protein that plays an essential role in the biogenesis of circular RNAs (circRNAs) which are produced by back-splicing circularization of pre-mRNAs. Within the nucleus, promotes circRNAs processing by stabilizing the regulatory elements residing in the flanking introns of the circularized exons. Plays thereby a role in the back-splicing of a subset of circRNAs (PubMed : 28625552). As a consequence, participates in a wide range of transcriptional and post-transcriptional processes. Binds to poly-U elements and AU-rich elements (AREs) in the 3'-UTR of target mRNAs (PubMed : 14731398). Upon viral infection, ILF3 accumulates in the cytoplasm and participates in the innate antiviral response (PubMed : 21123651, PubMed : 34110282). Mechanistically, ILF3 becomes phosphorylated and activated by the double-stranded RNA-activated protein kinase/PKR which releases ILF3 from cellular mature circRNAs. In turn, unbound ILF3 molecules are able to interact with and thus inhibit viral mRNAs (PubMed : 21123651, PubMed : 28625552).. (Microbial infection) Plays a positive role in HIV-1 virus production by binding to and thereby stabilizing HIV-1 RNA, together with ILF3.
See full target information ILF3
style
left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Molecular biology of the cell 35:ar62 PubMed38507240

2024

SARS-CoV-2 Orf6 is positioned in the nuclear pore complex by Rae1 to inhibit nucleocytoplasmic transport.

Applications

Unspecified application

Species

Unspecified reactive species

Tadashi Makio,Ke Zhang,Nicole Love,Fred D Mast,Xue Liu,Mohamed Elaish,Tom Hobman,John D Aitchison,Beatriz M A Fontoura,Richard W Wozniak

Cell death & disease 14:59 PubMed36697384

2023

Characterization of circSCL38A1 as a novel oncogene in bladder cancer via targeting ILF3/TGF-β2 signaling axis.

Applications

Unspecified application

Species

Unspecified reactive species

Peilong Li,Qi Mi,Suzhen Yan,Yan Xie,Zilian Cui,Shujun Zhang,Yifan Wang,Huiru Gao,Yunshan Wang,Juan Li,Lutao Du,Chuanxin Wang

Nucleic acids research 48:11626-11644 PubMed33130894

2020

SINEUP long non-coding RNA acts via PTBP1 and HNRNPK to promote translational initiation assemblies.

Applications

Unspecified application

Species

Unspecified reactive species

Naoko Toki,Hazuki Takahashi,Harshita Sharma,Matthew N Z Valentine,Ferdous-Ur M Rahman,Silvia Zucchelli,Stefano Gustincich,Piero Carninci

Journal of experimental & clinical cancer research 38:439 PubMed31747963

2019

Upregulation of ERp57 promotes clear cell renal cell carcinoma progression by initiating a STAT3/ILF3 feedback loop.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Liu,Jian-Xing Wang,Zi-Yuan Nie,Yue Wen,Xin-Ju Jia,Li-Na Zhang,Hui-Jun Duan,Yong-Hong Shi

Proceedings of the National Academy of Sciences of 114:4153-4158 PubMed28377514

2017

SMARCE1 is required for the invasive progression of in situ cancers.

Applications

WB

Species

Human

Ethan S Sokol,Yu-Xiong Feng,Dexter X Jin,Minu D Tizabi,Daniel H Miller,Malkiel A Cohen,Sandhya Sanduja,Ferenc Reinhardt,Jai Pandey,Daphne A Superville,Rudolf Jaenisch,Piyush B Gupta
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com