Anti-ILF3 antibody [EPR3627] - BSA and Azide free

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-ILF3 antibody [EPR3627] - BSA and Azide free (AB248492)

This data was developed using ab133354, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (2.6 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 dilution (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 dilution (2 µg/mL). DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ILF3 antibody [EPR3627] - BSA and Azide free (AB248492)

This data was developed using ab133354, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human colon cancer tissue sections labeling ILF3 with Purified ab133354 at 1/6000 dilution (0.09 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-ILF3 antibody [EPR3627] - BSA and Azide free (AB248492)

This data was developed using ab133354, the same antibody clone in a different buffer formulation. Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling ILF3 with Purified ab133354 at 1/50 dilution (10 μg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• WB

Unknown

Western blot - Anti-ILF3 antibody [EPR3627] - BSA and Azide free (AB248492)

This data was developed using ab133354, the same antibody clone in a different buffer formulation.

ab133354 recognizes only NF110 isoform of ILF3.

All lanes:

Western blot - Anti-ILF3 antibody [EPR3627] (<a href='/en-us/products/primary-antibodies/ilf3-antibody-epr3627-ab133354'>ab133354</a>) at 1/20000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

Raji (Human Burkitt's lymphoma B lymphocyte) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 14 kDa,63 kDa,95 kDa

Observed band size: 110 kDa,30 kDa,63 kDa,65 kDa

false

• WB

Unknown

Western blot - Anti-ILF3 antibody [EPR3627] - BSA and Azide free (AB248492)

This data was developed using ab133354, the same antibody clone in a different buffer formulation.

ab133354 recognizes only NF110 isoform of ILF3.

All lanes:

Western blot - Anti-ILF3 antibody [EPR3627] (<a href='/en-us/products/primary-antibodies/ilf3-antibody-epr3627-ab133354'>ab133354</a>) at 1/2000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 110 kDa

false