AB9664

Anti-ILP2 antibody

Name: Anti-ILP2 antibody (ab9664) | Abcam
Brand: Abcam Limited
SKU: AB9664

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(8 Publications)

Rabbit Polyclonal ILP2 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Synthetic Peptide within Human BIRC8 aa 1-50.

View Alternative Names

ILP2, BIRC8, Baculoviral IAP repeat-containing protein 8, Inhibitor of apoptosis-like protein 2, Testis-specific inhibitor of apoptosis, IAP-like protein 2, ILP-2

2 Images

Unknown

Immunocytochemistry - Anti-ILP2 antibody (AB9664)

ab9664 at 10μg/ml staining ILP2 in HepG2 cells by Immunocytochemistry.

Supplier Data

Western blot - Anti-ILP2 antibody (AB9664)

All lanes:

Western blot - Anti-ILP2 antibody (ab9664) at 1 µg/mL

Lane 1:

HepG2 cell lysate

Lane 2:

MOLT4 cell lysate

Predicted band size: 27 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human BIRC8 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q96P09

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

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Product details

Apoptosis, or programmed cell death, is related to many diseases, such as cancer. Apoptosis is triggered by a variety of stimuli including members in the TNF family and prevented by the inhibitor of apoptosis (IAP) proteins. IAP proteins form a conserved gene family including IAP, XIAP/ILP-1/MIHA, and Livin/KIAP that bind to and inhibits specific proteases. A novel member in the IAP protein family was recently identified and designated ILP-2 for IAP-like protein-2 (1). ILP-2 has high homology to ILP-1, but is encoded by a distinct gene that is solely expressed in testis of tested normal human tissues (1). ILP-2, unlike ILP-1, has no inhibitory effect on Fas and TNF induced apoptosis, but potently inhibits apoptosis induced by overexpression of Bax or by coexpression of caspase-9 with Apaf-1. ILP-2 interacts with the processed caspase-9. These results suggest that ILP-2 is a novel IAP family member with restricted specificity for caspase-9.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein ILP2 also known as Inhibitor of Apoptosis Protein 2 plays an important role mechanically by acting as an apoptotic suppressor. ILP2's estimated molecular mass is approximately 32 kDa. Expression mostly occurs in testicular tissues though traces are present in other tissues. ILP2 shares structural and functional similarities with other members of the inhibitor of apoptosis protein (IAP) family which allow it to interact closely with various cellular components.

Biological function summary

The protein functions by preventing cell death providing essential support for cellular survival. ILP2 is part of a complex that includes other IAPs and operates in the regulation of apoptosis. Its action directly inhibits caspases which are enzymes critical for the execution phase of cell apoptosis. This function is significant as it contributes to the balance between cell proliferation and death making ILP2 an important player in cellular homeostasis.

Pathways

The protein acts in critical signaling pathways related to cell survival and apoptosis like the NF-kB signaling pathway. ILP2 works in conjunction with proteins such as XIAP and c-IAP1 which participate in similar pathways contributing to protection against unwanted cell death. This involvement helps maintain normal cellular processes and supports proper functioning of tissues.

The protein ILP2 has been implicated in different malignancies including testicular cancer and some leukemia forms. ILP2 may lead to disease progression by preventing apoptosis which allows cancer cells to survive longer than they should. In these diseases ILP2 often interacts with other apoptosis inhibitors like XIAP and Survivin increasing the complexity of the therapeutic targets associated with cancer treatment.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Protects against apoptosis mediated by BAX.

See full target information BIRC8

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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Molecular medicine reports 25: PubMed35039877

2022

Proteomic analysis of inhibitor of apoptosis protein‑like protein‑2 on breast cancer cell proliferation.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Xiang,Lin Zhu,Zhiliang Zhang,Siyuan Wang,Ruxia Cui,Mingjun Xiang

Experimental and therapeutic medicine 22:1006 PubMed34345288

2021

Overexpression of HOXD8 inhibits the proliferation, migration and invasion of breast cancer cells by downregulating ILP2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoyun Wen,Yu Chen,Xiansong Fang

eLife 10: PubMed33620316

2021

Deficient spermiogenesis in mice lacking .

Applications

Unspecified application

Species

Unspecified reactive species

Feng Wang,Maria Gracia Gervasi,Ana Bošković,Fengyun Sun,Vera D Rinaldi,Jun Yu,Mary C Wallingford,Darya A Tourzani,Jesse Mager,Lihua Julie Zhu,Oliver J Rando,Pablo E Visconti,Lara Strittmatter,Ingolf Bach

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:10531-10546 PubMed32543747

2020

BML-111 treatment prevents cardiac apoptosis and oxidative stress in a mouse model of autoimmune myocarditis.

Applications

Unspecified application

Species

Unspecified reactive species

Rafael I Jaén,María Fernández-Velasco,Verónica Terrón,Sergio Sánchez-García,Carlos Zaragoza,Natalia Canales-Bueno,Almudena Val-Blasco,María Teresa Vallejo-Cremades,Lisardo Boscá,Patricia Prieto

The Journal of clinical investigation 130:2054-2068 PubMed32175920

2020

Neogenin neutralization prevents photoreceptor loss in inherited retinal degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jason Charish,Alireza P Shabanzadeh,Danian Chen,Patrick Mehlen,Santhosh Sethuramanujam,Hidekiyo Harada,Vera L Bonilha,Gautam Awatramani,Rod Bremner,Philippe P Monnier

Scientific reports 10:4625 PubMed32170161

2020

Tead transcription factors differentially regulate cortical development.

Applications

Unspecified application

Species

Unspecified reactive species

Tanzila Mukhtar,Jeremie Breda,Alice Grison,Zahra Karimaddini,Pascal Grobecker,Dagmar Iber,Christian Beisel,Erik van Nimwegen,Verdon Taylor

Experimental and therapeutic medicine 17:4687-4692 PubMed31086602

2019

Protective effects of Asiatic acid against pelvic inflammatory disease in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Dejia Kong,Ping Fu,Qin Zhang,Xian Ma,Ping Jiang

Oncology reports 40:2047-2055 PubMed30106449

2018

Inhibitor of apoptosis protein‑like protein‑2: A novel growth accelerator for breast cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lin Zhu,Weihua Zhou,Xidi Zhu,Siqi Xiang,Siyuan Wang,Yingxia Peng,Bangyun Lu,Peizhi Tang,Qian Chen,Mengjuan Wu,Xia Peng,Ziyu Chen,Ziyi Sun,Kunliang Yang,Mingjun Xiang,Dandan Yu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ILP2 antibody

Immunocytochemistry - Anti-ILP2 antibody (AB9664)

Western blot - Anti-ILP2 antibody (AB9664)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (8)

Product promise