Rabbit Recombinant Monoclonal ILT-4 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Images
![Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107), expandable thumbnail](https://content.abcam.com/products/images/ilt-4-antibody-epr22081-bsa-and-azide-free-ab243107--flow-cytometry-img17018.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107)")
![Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107), expandable thumbnail](https://content.abcam.com/products/images/ilt-4-antibody-epr22081-bsa-and-azide-free-ab243107--immunocytochemistry-immunofluorescence-img68613.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (AB243107)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
2 products for Alternative Version
Target data
Function
Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885).
Alternative names
CD85d, ILT4, LIR2, MIR10, LILRB2, Leukocyte immunoglobulin-like receptor subfamily B member 2, LIR-2, Leukocyte immunoglobulin-like receptor 2, CD85 antigen-like family member D, Immunoglobulin-like transcript 4, Monocyte/macrophage immunoglobulin-like receptor 10, ILT-4, MIR-10
Recommended products
Rabbit Recombinant Monoclonal ILT-4 antibody. Carrier free. Suitable for Flow Cyt, ICC/IF and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22081
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243107 is the carrier-free version of Anti-ILT-4 antibody [EPR22081] ab224701.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ILT-4 also known as Immunoglobulin-like transcript 4 is a transmembrane protein with a molecular weight of approximately 45 kDa. The protein is part of the LILR family and is sometimes referred to as LILRB2 protein. ILT-4 is mainly expressed on myeloid lineage cells including monocytes and dendritic cells. The expression of ILT-4 is important for the modulation of the immune response. Biotinylated ILT-4 and biotinylated LILRB2 versions are used frequently in research to study their interactions and functions.
- Biological function summary
ILT-4 plays an important role in the regulation of immune tolerance and inhibition. It is not part of a complex but interacts with other immune regulatory proteins. By binding to MHC class I molecules ILT-4 inhibits the activation of immune responses preventing over-activation and autoimmunity. The ILT-4 protein is critical for helping myeloid cells to maintain a balanced immune response often restricting unnecessary inflammation and promoting tolerance.
- Pathways
ILT-4 influences immune signaling pathways such as those that control T cell receptor signaling. ILT-4 interactions with proteins like CD85d and other LILRA6 proteins showcase its involvement in negative regulatory signaling pathways. These pathways are essential for maintaining immune homeostasis and limiting immune-mediated damage during infections or tissue injury.
- Associated diseases and disorders
ILT-4 is implicated in cancer and autoimmune diseases such as multiple sclerosis. In cancers ILT-4 modulates the immune environment allowing tumors to evade immune surveillance. In the context of autoimmune disorders aberrant ILT-4 expression or function can lead to insufficient immune regulation contributing to disease progression. CD85d and LILRB2 proteins are also associated with these conditions further influencing ILT-4's role in disease mechanisms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107), expandable thumbnail](https://content.abcam.com/products/images/ilt-4-antibody-epr22081-bsa-and-azide-free-ab243107--flow-cytometry-img17018.jpg "Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)")
Flow Cytometry - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)
Flow cytometric analysis of human primary peripheral blood mononuclear cell (PBMC) labeling ILT-4 with Anti-ILT-4 antibody [EPR22081] ab224701 at 1/500 (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat anti rabbit IgG (Dylight ® 488, ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or ILT-4 (Right).
Data shown were gated on viable cells.
The CD14 co-staining result observed is consistent with what has been described in the literature (PMID:10879687).
ILT-4 is expressed in monocytes and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ILT-4 antibody [EPR22081] ab224701).
![Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107), expandable thumbnail](https://content.abcam.com/products/images/ilt-4-antibody-epr22081-bsa-and-azide-free-ab243107--immunocytochemistry-immunofluorescence-img68613.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)")
Immunocytochemistry/ Immunofluorescence - Anti-ILT-4 antibody [EPR22081] - BSA and Azide free (ab243107)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labeling ILT-4 with Anti-ILT-4 antibody [EPR22081] ab224701 at 1/50 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing co-staining with CD14 and cytoplasmic staining in PBMC cells. The nuclear counterstain is DAPI (blue). Counterstained with an Alexa Fluor® 647 anti-human CD14 antibody at a 1/100 dilution (red).
The negative control is the secondary antibody only.
ILT-4 is expressed in monocytes (PMID:10879687) and CD14 is an established marker for monocytes and macrophages (PMID: 23382732).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ILT-4 antibody [EPR22081] ab224701).
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