AB176685

Anti-IMP3 antibody

Name: Anti-IMP3 antibody (ab176685) | Abcam
Brand: Abcam Limited
SKU: AB176685
Price: 535 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal IMP3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human IGF2BP3 aa 1-50.

View Alternative Names

IMP3, KOC1, VICKZ3, IGF2BP3, Insulin-like growth factor 2 mRNA-binding protein 3, IGF2 mRNA-binding protein 3, IMP-3, IGF-II mRNA-binding protein 3, KH domain-containing protein overexpressed in cancer, VICKZ family member 3, hKOC

2 Images

Supplier Data

Immunoprecipitation - Anti-IMP3 antibody (AB176685)

Detection of Human IMP3 by Western Blot of Immunoprecipitates.

Detection of IMP3 in Immunoprecipitates of HeLa whole cell lysates (1 mg for IP, 20% of IP loaded) using ab176685 at 6 μg/mg lysate for IP. For WB analysis of immunoprecipitated IMP3, ab176685 was used at 1 μg/ml. Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-IMP3 antibody (ab176685)

Predicted band size: 63 kDa

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Exposure time: 10s

Supplier Data

Western blot - Anti-IMP3 antibody (AB176685)

All lanes:

Western blot - Anti-IMP3 antibody (ab176685) at 0.04 µg/mL

Lane 1:

HeLa lysate at 50 µg

Lane 2:

HeLa lysate at 15 µg

Lane 3:

Jurkat lysate at 50 µg

Lane 4:

293T lysate at 50 µg

Predicted band size: 63 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human IGF2BP3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

O00425

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab176685 was affinity purified using an epitope specific to IMP3 immobilized on solid support.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IMP3 also known as IGF2BP3 is an oncofetal protein belonging to the insulin-like growth factor 2 mRNA-binding protein family. It has a molecular weight of around 65 kDa. IMP3 expresses in various tissues including embryonic tissues and several cancer types. In healthy adults the expression is minimal observed mostly in the testis placenta and some parts of the nervous system. It's significance in development and cancer makes it an important target for research.

Biological function summary

IMP3 plays a role in the regulation of mRNA stability localization and translation. It is a part of ribonucleoprotein complexes that facilitate these processes by binding to the 3' untranslated regions of target mRNAs. The protein influences the expression of genes involved in cell growth differentiation and migration. IMP3's ability to modulate mRNA fate contributes to cellular events essential for development and has implications in cancer progression.

Pathways

The involvement of IMP3 is significant in pathways like the insulin-like growth factor (IGF) signaling pathway and epithelial-mesenchymal transition (EMT). Within these pathways IMP3 modulates the activities of proteins such as IGF2 influencing cell proliferation and survival. Its participation in EMT is essential for processes like metastasis in cancer cells linking it to broader biological processes that govern cell movement and identity change.

High IMP3 expression associates with various cancers including pancreatic and ovarian cancers. It has been connected to the progression and poor prognosis of these cancers. In pancreatic cancer IMP3 can interact with proteins like IGF2 enhancing tumor growth and metastasis. This connection makes IMP3 a potential biomarker for cancer diagnostics and therapeutic targeting.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed : 29476152). Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed : 29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.

See full target information IGF2BP3

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 11:24357 PubMed34934095

2021

circRNA N6-methyladenosine methylation in preeclampsia and the potential role of N6-methyladenosine-modified circPAPPA2 in trophoblast invasion.

Applications

Unspecified application

Species

Unspecified reactive species

Yonggang Zhang,Hongling Yang,Yan Long,Yipeng Zhang,Ronggui Chen,Junzhu Shi,Jiying Chen

OncoTargets and therapy 12:3661-3670 PubMed31190868

2019

Insulin-like growth factor II mRNA-binding protein 3 promotes cell proliferation, migration and invasion in human glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Wu,Hongxin Ma,Guijun Qi,Fanyu Chen,Jiancheng Chu

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