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AB179807

Anti-IMP3 antibody [EPR12021-114]

  • BOND RX™ Validated
  • RabMAb
  • Recombinant
  • KO Validated
  • 20ul selling size
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(16 Publications )

Rabbit Recombinant Monoclonal IMP3 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 16 publications.
10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Unpurified ab179807 at 1 : 100 staining IMP3 antibody in human lung cancer tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human lung cancer tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Negative control : Immunohistochemical analysis of paraffin-embedded human adult kidney tissue labeling IMP3 with unpurified ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Unpurified ab179807 at 1 : 100 staining IMP3 antibody in human fetal kidney tissue by immunohistochemistry (FFPE). Immunohistochemical analysis of paraffin-embedded human fetal kidney tissue labeling IMP3 with ab179807 at 1/100 dilution. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IMP3 with purified ab179807 at 1/1000 dilution (0.10 µg/mL). Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Supplier Data

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Blocking and diluting buffer : 5% NFDM/TBST.

ab205606 was used as a loading control at 1/1000 dilution.

ab179807 was used at 1/1000 dilution.

Lanes 1 - 3:

Western blot - Anti-IGF2BP2/IMP-2 antibody [EPR6741(B)] (<a href='/en-us/products/primary-antibodies/igf2bp2-imp-2-antibody-epr6741b-ab124930'>ab124930</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution

Lane 1:

C-Myc/DDK-tagged full length Human IGF2BP1 recombinant protein at 0.01 µg

Lane 2:

C-Myc/DDK-tagged full length Human IGF2BP2 recombinant protein at 0.01 µg

Lane 3:

N-His-tagged full length Human IGF2BP3 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 66 kDa

Observed band size: 75 kDa

false

Exposure time: 180s

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Supplier Data

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

All lanes:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 3:

BxPC-3 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 63 kDa

Observed band size: 69 kDa

false

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Supplier Data

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Blocking and diluting buffer : 5% NFDM/TBST.

This antibody shows weak cross reactivity with IGF2BP2.

ab205606 was used as a loading control at 1/1000 dilution.

ab179807 was used at 1/1000 dilution.

Lanes 1 - 3:

Western blot - Anti-IGF2BP1/IMP1 antibody [EPR18791] (<a href='/en-us/products/primary-antibodies/igf2bp1-imp1-antibody-epr18791-ab184305'>ab184305</a>) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution

Lane 1:

C-Myc/DDK-tagged full length Human IGF2BP1 recombinant at 0.01 µg

Lane 2:

C-Myc/DDK-tagged full length Human IGF2BP2 recombinant protein at 0.01 µg

Lane 3:

N-His-tagged full length Human IGF2BP3 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Exposure time: 180s

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Lab

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Lane 1 : Wild-type HAP1 whole cell lysate (20 μg)
Lane 2 : IGF2BP3 (IMP3) knockout HAP1 whole cell lysate (20 μg)
Lane 3 : HeLa whole cell lysate (20 μg)

Lanes 1 - 3 : Merged signal (red and green). Green - Unpurified ab179807 observed at 70 kDa. Red - loading control, ab9484, observed at 37 kDa.

Unpurified ab179807 was shown to specifically react with IGF2BP3 (IMP3) in wild-type HAP1 cells as signal was lost in IGF2BP3 (IMP3) knockout cells. Wild-type and IGF2BP3 (IMP3) knockout samples were subjected to SDS-PAGE. ab179807 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/20,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807)

Predicted band size: 63 kDa

false

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Unknown

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Lanes 1 - 2:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution

Lane 3:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/10000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

Lanes 1 - 2:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lane 3:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 69 kDa

false

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)
  • WB

Supplier Data

Western blot - Anti-IMP3 antibody [EPR12021-114] (AB179807)

Blocking and diluting buffer concentration : 5% NFDM/TBST.

ab205606 DDDDK tag was used as a loading control at 1/1000 dilution.

All lanes:

Western blot - Anti-IMP3 antibody [EPR12021-114] (ab179807) at 1/1000 dilution

Lane 1:

C-Myc/DDK -tagged full length Human IGF2BP1 recombinant protein at 0.01 µg

Lane 2:

C-Myc/DDK -tagged full length Human IGF2BP2 recombinant protein at 0.01 µg

Lane 3:

N-His -tagged full length Human IGF2BP3 recombinant protein at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 63 kDa

Observed band size: 75 kDa

false

Exposure time: 180s

  • Carrier free

    Anti-IMP3 antibody [EPR12021-114] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12021-114

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p><strong>For unpurified use at 1/1000 - 1/5000.</strong></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "<p>See <a data-tabindex-counter=\"1\" data-tabindex-value=\"none\" href=\"../../../../protocols/ihc-antigen-retrieval-protocol\" tabindex=\"-1\">IHC antigen retrieval protocols</a>.</p><p><strong>For unpurified use at 1/100 - 1/250.</strong></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }
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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

IMP3 also known as IGF2BP3 is an oncofetal protein belonging to the insulin-like growth factor 2 mRNA-binding protein family. It has a molecular weight of around 65 kDa. IMP3 expresses in various tissues including embryonic tissues and several cancer types. In healthy adults the expression is minimal observed mostly in the testis placenta and some parts of the nervous system. It's significance in development and cancer makes it an important target for research.
Biological function summary

IMP3 plays a role in the regulation of mRNA stability localization and translation. It is a part of ribonucleoprotein complexes that facilitate these processes by binding to the 3' untranslated regions of target mRNAs. The protein influences the expression of genes involved in cell growth differentiation and migration. IMP3's ability to modulate mRNA fate contributes to cellular events essential for development and has implications in cancer progression.

Pathways

The involvement of IMP3 is significant in pathways like the insulin-like growth factor (IGF) signaling pathway and epithelial-mesenchymal transition (EMT). Within these pathways IMP3 modulates the activities of proteins such as IGF2 influencing cell proliferation and survival. Its participation in EMT is essential for processes like metastasis in cancer cells linking it to broader biological processes that govern cell movement and identity change.

High IMP3 expression associates with various cancers including pancreatic and ovarian cancers. It has been connected to the progression and poor prognosis of these cancers. In pancreatic cancer IMP3 can interact with proteins like IGF2 enhancing tumor growth and metastasis. This connection makes IMP3 a potential biomarker for cancer diagnostics and therapeutic targeting.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

RNA-binding factor that may recruit target transcripts to cytoplasmic protein-RNA complexes (mRNPs). This transcript 'caging' into mRNPs allows mRNA transport and transient storage. It also modulates the rate and location at which target transcripts encounter the translational apparatus and shields them from endonuclease attacks or microRNA-mediated degradation. Preferentially binds to N6-methyladenosine (m6A)-containing mRNAs and increases their stability (PubMed : 29476152). Binds to the 3'-UTR of CD44 mRNA and stabilizes it, hence promotes cell adhesion and invadopodia formation in cancer cells. Binds to beta-actin/ACTB and MYC transcripts. Increases MYC mRNA stability by binding to the coding region instability determinant (CRD) and binding is enhanced by m6A-modification of the CRD (PubMed : 29476152). Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs.
See full target information IGF2BP3

Alternative Names

IMP3, KOC1, VICKZ3, IGF2BP3, Insulin-like growth factor 2 mRNA-binding protein 3, IGF2 mRNA-binding protein 3, IMP-3, IGF-II mRNA-binding protein 3, KH domain-containing protein overexpressed in cancer, VICKZ family member 3, hKOC
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Publications (16)

Recent publications for all applications. Explore the full listand refine your search

Cell death & disease 16:684 PubMed41053003

2025

The IGF2BP3-FASN axis drives lipid metabolic reprogramming to promote brain colonization in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jingwei Li,Shumin Ouyang,Ziyou Lin,Keren Peng,Jiayu Yan,Minyuan Lu,Wen Ding,Jianshan Mo,Yingxue Su,Libin Wang,Peibin Yue,Jin-Jian Lu,Xiangchao Yao,Yandong Wang,Xiaolei Zhang

Molecular medicine (Cambridge, Mass.) 31:47 PubMed39910429

2025

Nitidine chloride inhibits the progression of hepatocellular carcinoma by suppressing IGF2BP3 and modulates metabolic pathways in an mA-dependent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Dan-Dan Xiong,Zhen-Dong Chen,Jian-di Li,Yu-Long Deng,Rong-Quan He,Zhi-Guang Huang,San-Qi An,Yi-Wu Dang,Gang Chen

Anticancer research 44:4951-4960 PubMed39477312

2024

Biomarker Study for Selecting Neoadjuvant Chemotherapy Regimens Based on Prognostic Prediction Using Gastric Cancer Biopsy Specimens from a Phase II Randomized Controlled Trial.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Oshima,Takaki Yoshikawa,Yohei Miyagi,Satoshi Morita,Michio Yamamoto,Kazuaki Tanabe,Kazuhiro Nishikawa,Yuichi Ito,Takanori Matsui,Yutaka Kimura,Toru Aoyama,Takashi Ogata,Haruhiko Cho,Akira Tsuburaya,Junichi Sakamoto

Journal of translational medicine 22:900 PubMed39367493

2024

Multi-omics analysis unveils the predictive value of IGF2BP3/SPHK1 signaling in cancer stem cells for prognosis and immunotherapeutic response in muscle-invasive bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yaobang Wang,Wuyue Song,Chao Feng,Shulin Wu,Zezu Qin,Tao Liu,Yu Ye,Rong Huang,Yuanliang Xie,Zhong Tang,Qiuyan Wang,Tianyu Li

Molecular medicine (Cambridge, Mass.) 30:64 PubMed38760723

2024

IGF2BP3 promotes glutamine metabolism of endometriosis by interacting with UCA1 to enhances the mRNA stability of GLS1.

Applications

Unspecified application

Species

Unspecified reactive species

Honglin Wang,Yingying Cao,Yanling Gou,Hao Wang,Zongwen Liang,Qiong Wu,Jiahuan Tan,Jinming Liu,Zhi Li,Jing Cui,Huiyan Zhang,Zongfeng Zhang

World journal of oncology 15:100-113 PubMed38274714

2024

Inhibiting the mA Reader IGF2BP3 Suppresses Ovarian Cancer Cell Growth via Regulating PLAGL2 mRNA Stabilization.

Applications

Unspecified application

Species

Unspecified reactive species

Tian Tian Dai,Yi Ze Li,Hui Ting Hu,Yong Mei Zhao,Hong Yan Peng,Wen Dong Bai,Jing Wen Wang

Cell death & disease 14:827 PubMed38092752

2023

Fasting regulates mitochondrial function through lncRNA PRKCQ-AS1-mediated IGF2BPs in papillary thyroid carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoping Zhang,Yong Zhong,Lin Liu,Chengyou Jia,Haidong Cai,Jianshe Yang,Bo Wu,Zhongwei Lv

CNS neuroscience & therapeutics 29:3756-3773 PubMed37309294

2023

Exosomal miR-6733-5p mediates cross-talk between glioblastoma stem cells and macrophages and promotes glioblastoma multiform progression synergistically.

Applications

Unspecified application

Species

Unspecified reactive species

Shilu Huang,Liang Liu,Zhipeng Xu,Xinglei Liu,Anyi Wu,Xiaopei Zhang,Zengyang Li,Suwen Li,Yongdong Li,Jiaqi Yuan,Shan Cheng,Haoran Li,Jun Dong

Translational cancer research 12:992-1005 PubMed37180667

2023

N-methyladenosine reader as a prognostic Biomarker contribute to malignant progression of glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Zheng,Shenggang Li,Ju Yu,Chungang Dai,Suji Yan,Gang Chen,Chao Sun

BMC cancer 23:116 PubMed36732736

2023

IGF2BP3 overexpression predicts poor prognosis and correlates with immune infiltration in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Huang,Lizhen Zhu,Haoxuan Huang,Yuanyuan Li,Gongxian Wang,Cheng Zhang
View all publications
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